| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12637-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05935-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB17572-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97309SS | |||
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunocytochemistry-Immunofluorescence-NBP2-67865-img0003.jpg "Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Analysis of paraffin-embedded human kidney tissue using anti-IL1 beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunohistochemistry-Paraffin-NBP2-67865-img0004.jpg)
![Flow Cytometry: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Analysis of Hela cells with IL1 beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Flow-Cytometry-NBP2-67865-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunocytochemistry-Immunofluorescence-NBP2-67865-img0002.jpg)
![Western Blot: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32596) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-27820241633128.jpg "Western Blot: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32596) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32596) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-298202413585565.jpg)
![Immunohistochemistry: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32596, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-29820241430416.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunocytochemistry analysis of HeLa cells labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-29202410334040.jpg)
![Immunocytochemistry/Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609] - Staining PCK1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PCK1-Antibody-JU84-39-Immunocytochemistry-Immunofluorescence-NBP2-75609-img0003.jpg "Immunocytochemistry/Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609] - Staining PCK1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: PCK1 Antibody (JU84-39) [NBP2-75609] - Analysis of paraffin-embedded human kidney tissue using anti-PCK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCK1-Antibody-JU84-39-Immunohistochemistry-Paraffin-NBP2-75609-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609] - Staining PCK1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PCK1-Antibody-JU84-39-Immunocytochemistry-Immunofluorescence-NBP2-75609-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609] - Staining PCK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PCK1-Antibody-JU84-39-Immunocytochemistry-Immunofluorescence-NBP2-75609-img0002.jpg)
![Immunohistochemistry-Paraffin: PCK1 Antibody (JU84-39) [NBP2-75609] - Analysis of paraffin-embedded human liver tissue using anti-PCK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCK1-Antibody-JU84-39-Immunohistochemistry-Paraffin-NBP2-75609-img0004.jpg)
![Western Blot: dUTPase Antibody (JE66-11) [NBP3-32281] - Western blot analysis of dUTPase on different lysates with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: A549 cell lysate (15 ug/Lane) Lane 3: Jurkat cell lysate (15 ug/Lane) Lane 4: LO2 cell lysate (15 ug/Lane) Lane 5: HepG2 cell lysate (15 ug/Lane) Lane 6: K-562 cell lysate (15 ug/Lane) Lane 7: HEK-293 cell lysate (15 ug/Lane) Lane 8: A431 cell lysate (15 ug/Lane) Lane 9: Human liver tissue lysate (30 ug/Lane) Predicted band size: 27 kDa Observed band size: 20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32281) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32281_rabbit-dutpase-mab-je66-11-278202415295584.jpg "Western Blot: dUTPase Antibody (JE66-11) [NBP3-32281] - Western blot analysis of dUTPase on different lysates with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: A549 cell lysate (15 ug/Lane) Lane 3: Jurkat cell lysate (15 ug/Lane) Lane 4: LO2 cell lysate (15 ug/Lane) Lane 5: HepG2 cell lysate (15 ug/Lane) Lane 6: K-562 cell lysate (15 ug/Lane) Lane 7: HEK-293 cell lysate (15 ug/Lane) Lane 8: A431 cell lysate (15 ug/Lane) Lane 9: Human liver tissue lysate (30 ug/Lane) Predicted band size: 27 kDa Observed band size: 20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32281) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: dUTPase Antibody (JE66-11) [NBP3-32281] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32281) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32281_rabbit-dutpase-mab-je66-11-29820241430414.jpg)
![Immunocytochemistry/ Immunofluorescence: dUTPase Antibody (JE66-11) [NBP3-32281] - Immunocytochemistry analysis of HCT116 cells labeling dUTPase with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32281_rabbit-dutpase-mab-je66-11-29202410334031.jpg)
![Flow Cytometry: dUTPase Antibody (JE66-11) [NBP3-32281] - Flow cytometric analysis of HCT116 cells labeling dUTPase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32281, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32281_rabbit-dutpase-mab-je66-11-29202416372165.jpg)
![Western Blot: ECHDC1 Antibody [NBP3-12637] - Lane A: U-251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/ECHDC1-Antibody-Western-Blot-NBP3-12637-img0001.jpg "Western Blot: ECHDC1 Antibody [NBP3-12637] - Lane A: U-251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: ECHDC1 Antibody [NBP3-12637] - Lane A:0.5 mg U-251MG Whole Cell Lysate4 uL anti-ECHDC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ECHDC1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :34 kDa](http://images.novusbio.com/fullsize/ECHDC1-Antibody-Immunoprecipitation-NBP3-12637-img0002.jpg)
![Immunohistochemistry-Paraffin: ECHDC1 Antibody - BSA Free [NBP3-12637] - Immunochemical staining of human ECHDC1 in human kidney with NBP3-12637 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-12637_rabbit-polyclonal-echdc1-antibody-235202521451763.png)
![Immunocytochemistry/Immunofluorescence: ECHDC1 Antibody [NBP3-12637] - Staining of ECHDC1 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12637 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-12637_rabbit-polyclonal-echdc1-antibody-23520251628176.png)
![Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: TF-1 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 50-75 kDa Exposure time: 1 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-278202416285246.jpg "Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: TF-1 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 50-75 kDa Exposure time: 1 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Granulin Antibody (JE37-73) [NBP3-32371] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-298202414262694.jpg)
![Immunocytochemistry/ Immunofluorescence: Granulin Antibody (JE37-73) [NBP3-32371] - Immunocytochemistry analysis of THP-1 cells labeling Granulin with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-2920241114832.jpg)
![Flow Cytometry: Granulin Antibody (JE37-73) [NBP3-32371] - Flow cytometric analysis of THP-1 cells labeling Granulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32371, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-29202416453258.jpg)
![Western Blot: Phospholipase C delta 1 Antibody [NBP3-05935] - Lane A: HeLa Whole Cell LysateLane B: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:88 kDaObserved band size:83 kDa](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Western-Blot-NBP3-05935-img0001.jpg "Western Blot: Phospholipase C delta 1 Antibody [NBP3-05935] - Lane A: HeLa Whole Cell LysateLane B: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:88 kDaObserved band size:83 kDa")
![Immunohistochemistry-Paraffin: Phospholipase C delta 1 Antibody [NBP3-05935] - Staining of human PLCD1 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Immunohistochemistry-Paraffin-NBP3-05935-img0002.jpg)
![Immunoprecipitation: Phospholipase C delta 1 Antibody [NBP3-05935] - Lane A:0.5 mg NIH3T3 Whole Cell Lysate4 uL anti-PLCD1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PLCD1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 86 kDaObserved band size :90 kDa](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Immunoprecipitation-NBP3-05935-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Phospholipase C delta 1 Antibody [NBP3-05935] - Staining of PLCD1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-05935 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-05935_rabbit-polyclonal-phospholipase-c-delta-1-antibody-235202511494035.png)
![Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32597, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-298202414304127.jpg "Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32597, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32597) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-298202414333672.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunocytochemistry analysis of HeLa cells labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-29202410334014.jpg)
![Western Blot: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/100,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Saos-2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-3920248485018.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of ALDH1A1 expression in transfected 293T cell line by ALDH1A1 monoclonal antibody (M05), clone 1G6. Lane 1: ALDH1A1 transfected lysate (Predicted MW: 54.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-1G6-Western-Blot-H00000216-M05-img0004.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of ALDH1A1 expression in transfected 293T cell line by ALDH1A1 monoclonal antibody (M05), clone 1G6. Lane 1: ALDH1A1 transfected lysate (Predicted MW: 54.9 KDa). Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of monoclonal antibody to ALDH1A1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-1G6-Immunohistochemistry-Paraffin-H00000216-M05-img0001.jpg)
![Immunoprecipitation: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of ALDH1A1 transfected lysate using anti-ALDH1A1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ALDH1A1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-1G6-Immunoprecipitation-H00000216-M05-img0002.jpg)
![ELISA: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Detection limit for recombinant GST tagged ALDH1A1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-1G6-ELISA-H00000216-M05-img0005.jpg)
![Western Blot: MVD Antibody [NBP1-33050] - A. 30 ug 293T whole lysate/extract, B. 30 ug A431 whole cell lysate/extract, C. 30 ug HeLa whole cell lysate/extract. D. 30 ug A37C whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/MVD-Antibody-Western-Blot-NBP1-33050-img0006.jpg "Western Blot: MVD Antibody [NBP1-33050] - A. 30 ug 293T whole lysate/extract, B. 30 ug A431 whole cell lysate/extract, C. 30 ug HeLa whole cell lysate/extract. D. 30 ug A37C whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: MVD Antibody [NBP1-33050] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MVD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33050-img0003.jpg)
![Immunohistochemistry-Paraffin: MVD Antibody [NBP1-33050] - Paraffin-embedded MCF7 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MVD-Antibody-Immunohistochemistry-Paraffin-NBP1-33050-img0004.jpg)
![Western Blot: MVD Antibody [NBP1-33050] - Sample (50 ug of whole cell lysate) A: mouse Liver 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MVD-Antibody-Western-Blot-NBP1-33050-img0005.jpg)
![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (972737) [MAB17572] - Antibody was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-972737-Immunohistochemistry-Paraffin-MAB17572-img0002.jpg "Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (972737) [MAB17572] - Antibody was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue.")
![Immunohistochemistry: PAD4 Antibody [NBP1-97309] - PAD4 antibody was tested in mouse liver using DAB with hematoxylin counterstain. The image on the right has been blocked with the immunizing peptide.](http://images.novusbio.com/fullsize/PAD4-Antibody-Immunohistochemistry-NBP1-97309-img0001.jpg "Immunohistochemistry: PAD4 Antibody [NBP1-97309] - PAD4 antibody was tested in mouse liver using DAB with hematoxylin counterstain. The image on the right has been blocked with the immunizing peptide.")