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![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NBP2-48532] - Staining of human cell line Hep G2 shows localization to lipid droplets. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48532-img0006.jpg "Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NBP2-48532] - Staining of human cell line Hep G2 shows localization to lipid droplets. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody [NBP2-48532] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-Immunohistochemistry-Paraffin-NBP2-48532-img0011.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody [NBP2-48532] - Staining of human cerebellum shows no postivity in neurons as expected.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-Immunohistochemistry-Paraffin-NBP2-48532-img0007.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody [NBP2-48532] - Staining of human liver shows moderate cytoplasmic psoticity in hepatocytes.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-Immunohistochemistry-Paraffin-NBP2-48532-img0008.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody [NBP2-48532] - Staining of human lactating breast shows strong positivity in glandular cellls.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-Immunohistochemistry-Paraffin-NBP2-48532-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot-Perilipin-2/ADFP Antibody [NBP2-48532] -Analysis in human cell lines RT-4 and U-251MG using Anti-PLIN2 antibody. Corresponding PLIN2 RNA-seq data are presented for the same cell lines.](http://images.novusbio.com/fullsize/antibody/nbp2-48532_rabbit-polyclonal-perilipin-2-adfp-antibody-western-blot-285202412135..jpg)
![Western Blot: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP1C gamma antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1C-gamma-Antibody-Western-Blot-NBP1-32858-img0012.jpg "Western Blot: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP1C gamma antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1C-gamma-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32858-img0008.jpg)
![Immunohistochemistry-Paraffin: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - SAS xenograft, using PPP1CC antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1C-gamma-Antibody-Immunohistochemistry-Paraffin-NBP1-32858-img0009.jpg)
![Western Blot: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1C-gamma-Antibody-Western-Blot-NBP1-32858-img0010.jpg)
![Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP2-20917-img0002.jpg "Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody [N1C3] (NBP2-20917) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20917_rabbit-polyclonal-xbp1-antibody-western-blot-2611202518211016.jpg)
![Western Blot: ECH1 Antibody [NBP2-16261] - Wild-type (WT) and ECH1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ECH1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ECH1-Antibody-Western-Blot-NBP2-16261-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ECH1 Antibody [NBP2-16261] - Wild-type (WT) and ECH1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ECH1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ECH1 Antibody [NBP2-16261] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ECH1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/ECH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16261-img0003.jpg)
![Immunohistochemistry-Paraffin: ECH1 Antibody [NBP2-16261] - ECH1 antibody detects ECH1 protein at mitochondrion on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. ECH1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECH1-Antibody-Immunohistochemistry-Paraffin-NBP2-16261-img0004.jpg)
![Western Blot: ECH1 Antibody [NBP2-16261] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ECH1-Antibody-Western-Blot-NBP2-16261-img0001.jpg)
![Western Blot: ECH1 Antibody [NBP2-16261] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ECH1-Antibody-Western-Blot-NBP2-16261-img0002.jpg)
![Western Blot: Lipoprotein Lipase/LPL Antibody [NBP1-32215] - NIH-3T3 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LPL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Lipoprotein-Lipase-LPL-Antibody-Western-Blot-NBP1-32215-img0009.jpg "Western Blot: Lipoprotein Lipase/LPL Antibody [NBP1-32215] - NIH-3T3 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LPL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: Lipoprotein Lipase/LPL Antibody [NBP1-32215] - Paraformaldehyde-fixed mouse ESC D3, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Lipoprotein-Lipase-LPL-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32215-img0005.jpg)
![Immunohistochemistry-Paraffin: Lipoprotein Lipase/LPL Antibody [NBP1-32215] - Mouse white adipocyte. LPL stained by LPL antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Lipoprotein-Lipase-LPL-Antibody-Immunohistochemistry-Paraffin-NBP1-32215-img0010.jpg)
![Western Blot: Lipoprotein Lipase/LPL Antibody [NBP1-32215] - Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LPL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/Lipoprotein-Lipase-LPL-Antibody-Western-Blot-NBP1-32215-img0007.jpg)
![Western Blot: Ketohexokinase Antibody [NBP1-32778] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Western-Blot-NBP1-32778-img0009.jpg "Western Blot: Ketohexokinase Antibody [NBP1-32778] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: ketohexokinase Antibody [NBP1-32778] - Sample (20 ug of whole cell lysate) A: mouse Liver 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/ketohexokinase-Antibody-Western-Blot-NBP1-32778-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Ketohexokinase Antibody [NBP1-32778] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Western-Blot-NBP1-32778-img0007.jpg)
![Immunohistochemistry-Paraffin: ketohexokinase Antibody [NBP1-32778] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-32778-img0002.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-32778] - Paraffin-embedded mouse kidney. Ketohexokinase antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-32778-img0005.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-32778] - Ketohexokinase antibody [N1C3] detects Ketohexokinase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Ketohexokinase stained by Ketohexokinase antibody [N1C3] (NBP1-32778) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32778_rabbit-polyclonal-ketohexokinase-antibody-91020242021965.jpg)
![P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunohistochemistry-paraffin-1411202518431319.jpg "<b>Orthogonal Strategies Validation. </b>P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Orthogonal Strategies Validation. </b>P2Y2 antibody [HL2801] detects P2Y2 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunocytochemistry-immunofluorescence-141120251830212.jpg)
![P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat small intestine.P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunohistochemistry-paraffin-141120251839418.jpg)
![P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunohistochemistry-paraffin-141120251839412.jpg)
![P2Y2 antibody [HL2801] detects P2Y2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunohistochemistry-paraffin-141120251839317.jpg)
![P2Y2 antibody [HL2801] detects P2Y2 protein by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:100.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunocytochemistry-immunofluorescence-141120251824331.jpg)
![P2Y2 antibody [HL2801] detects P2Y2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunohistochemistry-paraffin-1411202518334522.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78/HSPA5 protein using Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318). Primary Antibody: Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318) at 1:1000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-54318-img0008.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78/HSPA5 protein using Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318). Primary Antibody: Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-54318] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 (Bip) Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54318-img0006.jpg)
![Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Western-Blot-NBP3-16840-img0005.jpg "Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of mouse eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0004.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Rat retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0001.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Mouse retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0002.jpg)
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of rat eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of paraffin-embedded rat eye using RPE65 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16840_rabbit-rpe65-mab-5x1f6-52202511361295.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-54559-img0002.jpg "Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).")
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (4C4.9 + S100B/1012)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunohistochemistry-Paraffin-NBP2-54559-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-54559-img0001.jpg)
![Western Blot: S100B Antibody (SPM354) - Azide and BSA Free [NBP2-54399] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Azide-and-BSA-Free-Western-Blot-NBP2-54399-img0002.jpg "Western Blot: S100B Antibody (SPM354) - Azide and BSA Free [NBP2-54399] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).")
![Immunohistochemistry-Paraffin: S100B Antibody (SPM354) - Azide and BSA Free [NBP2-54399] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54399-img0001.jpg)
![Western Blot: PKM2 Antibody (7B2) [NBP3-15230] - Positive WB detected in: SH-SY5Y whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue. All lanes: PKM2 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 59, 57 KDa. Observed band size: 58 KDa.](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Western-Blot-NBP3-15230-img0005.jpg "Western Blot: PKM2 Antibody (7B2) [NBP3-15230] - Positive WB detected in: SH-SY5Y whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue. All lanes: PKM2 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 59, 57 KDa. Observed band size: 58 KDa.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (7B2) [NBP3-15230] - Immunofluorescence staining of Hela Cells with at PKM2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Immunocytochemistry-Immunofluorescence-NBP3-15230-img0002.jpg)
![Immunohistochemistry: PKM2 Antibody (7B2) [NBP3-15230] - IHC image of PKM2 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Immunohistochemistry-NBP3-15230-img0004.jpg)
![Flow Cytometry: PKM2 Antibody (7B2) [NBP3-15230] - Overlay histogram showing HepG2 cells stained with PKM2 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Flow-Cytometry-NBP3-15230-img0001.jpg)
![Immunoprecipitation: PKM2 Antibody (7B2) [NBP3-15230] - Immunoprecipitating PKM2 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of PKM2 antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PKM2 antibody (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Immunoprecipitation-NBP3-15230-img0003.jpg)