| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90222-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89518-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97369-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89207-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00295-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00064-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12036-0.025mg | |||
![Western Blot: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - Anti-PSPH rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:25 kDa. Observed band size:27 kDa](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-001-Western-Blot-NBP2-90222-img0004.jpg "Western Blot: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - Anti-PSPH rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:25 kDa. Observed band size:27 kDa")
![Immunoprecipitation: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - PSPH was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg HepG2 Whole Cell Lysate. 0.5 uL anti-PSPH rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PSPH rabbit monoclonal antibody,at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 25 kDa. Observed band size: 25 kDa.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-001-Immunoprecipitation-NBP2-90222-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - Immunofluorescence staining of Human PSPH in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90222 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-90222_rabbit-monoclonal-phosphoserine-phosphatase-antibody-001-235202511444476.png)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518] - Staining of human CA4 in human kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to renal tubules.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IV-CA4-Antibody-039-Immunohistochemistry-Paraffin-NBP2-89518-img0002.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518] - Staining of human CA4 in human kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to renal tubules.")
![Flow Cytometry: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518] - Human primary renal tubular epithelial cells stained with carbonic Anhydrase IV/CA4 antibody conjugated to Alexa Fluor 488 (NBP2-89518AF488). Cells were immediately fixed with 4% PFA after detachment from culture plates and prior to staining. Final Ab concentration was 1:50. Image from verified customer review.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IV-CA4-Antibody-039-Flow-Cytometry-NBP2-89518-img0003.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518] - Staining of human CA4 in human lung with rabbit monoclonal antibody (1:200). Positive staining was localized to pulmonary capillaries.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IV-CA4-Antibody-039-Immunohistochemistry-Paraffin-NBP2-89518-img0001.jpg)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP2-97369] - Immunochemical staining of human Derlin 1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-97369-img0002.jpg "Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP2-97369] - Immunochemical staining of human Derlin 1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Derlin 1 Antibody - BSA Free [NBP2-97369] - Immunochemical staining of human Derlin 1 in human brain with NBP2-97369 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-97369_rabbit-polyclonal-derlin-1-antibody-235202514492372.png)
![Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NBP2-97369] - Immunofluorescence staining of Derlin 1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97369 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97369_rabbit-polyclonal-derlin-1-antibody-23520252135188.png)
![Western Blot: ATF6 Antibody (8D3-R) [NBP3-32054] - Western blot analysis of ATF6 on different lysates with Mouse anti-ATF6 antibody (NBP3-32054) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: RAW264.7 cell lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (20 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32054) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32054_mouse-atf6-mab-8d3-r-278202416331270.jpg "Western Blot: ATF6 Antibody (8D3-R) [NBP3-32054] - Western blot analysis of ATF6 on different lysates with Mouse anti-ATF6 antibody (NBP3-32054) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: RAW264.7 cell lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (20 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32054) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody (8D3-R) [NBP3-32054] - Immunocytochemistry analysis of HeLa cells labeling ATF6 with Mouse anti-ATF6 antibody (NBP3-32054) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ATF6 antibody (NBP3-32054) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32054_mouse-atf6-mab-8d3-r-29202410234226.jpg)
![Western Blot: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32721) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32721_rabbit-pyruvate-carboxylase-mab-je40-24-278202416331212.jpg "Western Blot: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32721) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32721) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32721_rabbit-pyruvate-carboxylase-mab-je40-24-2982024142096.jpg)
![Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89207 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89207_rabbit-monoclonal-4ebp1-antibody-007-23520251154563.png "Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89207 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.")
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP3-00295] - Immunochemical staining of human FHIT in human lung cancer with NBP3-00295 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00295_rabbit-polyclonal-fhit-antibody-235202514331511.png "Immunohistochemistry-Paraffin: FHIT Antibody [NBP3-00295] - Immunochemical staining of human FHIT in human lung cancer with NBP3-00295 (1:1000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP3-00295] - Immunochemical staining of human FHIT in human breast carcinoma with NBP3-00295 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00295_rabbit-polyclonal-fhit-antibody-23520251633505.png)
![Immunocytochemistry/ Immunofluorescence: FHIT Antibody [NBP3-00295] - Immunofluorescence staining of FHIT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00295 (1:1000) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp3-00295_rabbit-polyclonal-fhit-antibody-23520251129352.png)
![Western Blot: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lane 4: HeLa cell lysate Lane 5: HepG2 cell lysate Lane 6: K-562 cell lysate Lane 7: A549 cell lysate Lane 8: Raji cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33131) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33131_mouse-nm23-h1-mab-13c1-r-288202414191531.jpg "Western Blot: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lane 4: HeLa cell lysate Lane 5: HepG2 cell lysate Lane 6: K-562 cell lysate Lane 7: A549 cell lysate Lane 8: Raji cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33131) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Immunocytochemistry analysis of MCF7 cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33131_mouse-nm23-h1-mab-13c1-r-2920241192318.jpg)
![Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody at 1:500 dilution. Lane A: ADP-Sugar Pyrophosphatase/NUDT5 knockout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/ADP-Sugar-Pyrophosphatase-NUDT5-Antibody-Western-Blot-NBP3-00064-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody at 1:500 dilution. Lane A: ADP-Sugar Pyrophosphatase/NUDT5 knockout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa")
![<b>Genetic Strategies Validation. </b>Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/ADP-Sugar-Pyrophosphatase-NUDT5-Antibody-Western-Blot-NBP3-00064-img0006.jpg)
![Immunoprecipitation: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - ADP-Sugar Pyrophosphatase/NUDT5 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 2 ul anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/ADP-Sugar-Pyrophosphatase-NUDT5-Antibody-Immunoprecipitation-NBP3-00064-img0004.jpg)
![Western Blot: PMP70 Antibody (JE39-00) [NBP3-32833] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32833) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32833_rabbit-pmp70-mab-je39-00-288202412461622.jpg "Western Blot: PMP70 Antibody (JE39-00) [NBP3-32833] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32833) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (JE39-00) [NBP3-32833] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32833) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32833_rabbit-pmp70-mab-je39-00-298202414304140.jpg)
![Western Blot: iNOS [p Tyr1055] Antibody [NBP3-23217] - Western blot analysis of mouse macrophages (J774A.1) treated with LPS (1ug/ml) for 18 hrs followed by pervanadate (1 mM) for 30 min. (lanes 1, 3 & 5). The blots were then treated with akaline phosphatase (lanes 2, 4 & 6). Blots were probed with rabbit polyclonal anti-inducible Nitric Oxide Synthase (iNOS) (lanes 1 & 2), anti-iNOS (Tyr-1055) (lanes 3 & 4), and mouse monoclonal anti-iNOS (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23217_rabbit-inos-p-tyr1055-pab-19920231759440.jpg "Western Blot: iNOS [p Tyr1055] Antibody [NBP3-23217] - Western blot analysis of mouse macrophages (J774A.1) treated with LPS (1ug/ml) for 18 hrs followed by pervanadate (1 mM) for 30 min. (lanes 1, 3 & 5). The blots were then treated with akaline phosphatase (lanes 2, 4 & 6). Blots were probed with rabbit polyclonal anti-inducible Nitric Oxide Synthase (iNOS) (lanes 1 & 2), anti-iNOS (Tyr-1055) (lanes 3 & 4), and mouse monoclonal anti-iNOS (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: iNOS [p Tyr1055] Antibody [NBP3-23217] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23217_rabbit-inos-p-tyr1055-pab-19920231992812.jpg)
![Western Blot: CD98 Antibody (RL388) - Chimeric [NBP3-12036] - Mouse fetal liver lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 (NBP3-12036) at 0.05 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD98-Antibody-RL388-Chimeric-Western-Blot-NBP3-12036-img0001.jpg "Western Blot: CD98 Antibody (RL388) - Chimeric [NBP3-12036] - Mouse fetal liver lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 (NBP3-12036) at 0.05 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: CD98 Antibody (RL388) - Chimeric - Azide and BSA Free [NBP3-12036] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with NBP3-12036 at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12036, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12036_rabbit-monoclonal-cd98-antibody-rl388-chimeric-74202522141236.png)