| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52535-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50300-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52534-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52556-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05183-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89736-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00324-50ul | |||
![Western Blot: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Western blot analysis of extracts of HeLa cells, using Caveolin-1antibody (NBP3-15615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-9I9J8-Western-Blot-NBP3-15615-img0017.jpg "Western Blot: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Western blot analysis of extracts of HeLa cells, using Caveolin-1antibody (NBP3-15615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Human colon using Caveolin-1 Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-immunohistochemistry-paraffin-184202410253..jpg)
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody (9I9J8) [NBP3-15615] -Human lung using Caveolin-1 Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-immunohistochemistry-paraffin-184202410355..jpg)
![Western Blot: Caveolin-1 Antibody (10Y5L2) [Caveolin-1] - Western blot analysis of various lysates, using Caveolin-1 Rabbit mAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-5220251731106.jpg)
![Western Blot: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Analysis using PLCG2 mAb against HEK293 (1) and PLCG2 (AA: 826-985)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-1E10C11-Western-Blot-NBP2-52535-img0002.jpg "Western Blot: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Analysis using PLCG2 mAb against HEK293 (1) and PLCG2 (AA: 826-985)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Immunohistochemical analysis of paraffin-embedded Hela tissues using PLC-gamma 2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-1E10C11-Immunohistochemistry-Paraffin-NBP2-52535-img0004.jpg)
![Western Blot: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Analysis using PLCG2 mAb against human PLCG2 (AA: 826-985) recombinant protein. (Expected MW is 44.6 kDa)](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-1E10C11-Western-Blot-NBP2-52535-img0001.jpg)
![ELISA: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-1E10C11-ELISA-NBP2-52535-img0003.jpg)
![Western Blot: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis using PLCG2 mouse mAb against A431 cell lysate.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-3A8B6-Western-Blot-NBP2-52534-img0005.jpg "Western Blot: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis using PLCG2 mouse mAb against A431 cell lysate.")
![Immunocytochemistry/Immunofluorescence: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Immunofluorescence analysis of Hela cells using PLCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-3A8B6-Immunofluorescence-NBP2-52534-img0002.jpg)
![Flow Cytometry: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis of Hela cells using PLCG2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-3A8B6-Flow-Cytometry-NBP2-52534-img0001.jpg)
![ELISA: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-3A8B6-ELISA-NBP2-52534-img0004.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0003.jpg "Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)")
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of MCF-7 cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0007.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against HEK293 (1) and SELL (AA:83-186)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of Hela cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0006.jpg)
![ELISA: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-ELISA-NBP2-52556-img0005.jpg)
![Western Blot: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1I3.](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Western-Blot-NBP2-71525-img0001.jpg "Western Blot: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1I3.")
![Immunohistochemistry: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71525-img0004.jpg)
![Immunohistochemistry: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71525-img0002.jpg)
![Immunohistochemistry: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71525-img0003.jpg)
![Immunohistochemistry: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71525-img0005.jpg)
![Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression 2 days after the introduction of ischemic-reperfusion injured mouse kidney;detected with anti-mouse NGAL antibody at dilution of 1:1000. Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-29-BSA-Free-Immunohistochemistry-NBP1-05183-img0006.jpg "Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression 2 days after the introduction of ischemic-reperfusion injured mouse kidney;detected with anti-mouse NGAL antibody at dilution of 1:1000. Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.")
![Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression in the ischemia-reperfusion injured mouse kidney; detected with anti-mouse NGAL antibody at dilution of 1:1000. Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-29-BSA-Free-Immunohistochemistry-NBP1-05183-img0005.jpg)
![ELISA: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - The figure illustrates the calibration curve of a sandwich assay with NBP2-21746 as the capture antibody and NBP1-05183B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-29-BSA-Free-ELISA-NBP1-05183-img0007.jpg)
![Western Blot: NM23-H1 Antibody (13C2-R) [NBP3-33132] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: Raji cell lysate (20 ug/Lane) Lane 5: HeLa cell lysate (20 ug/Lane) Lane 6: K-562 cell lysate (20 ug/Lane) Lane 7: A549 cell lysate (20 ug/Lane) Lane 8: A431 cell lysate (20 ug/Lane) Lane 9: HepG2 cell lysate (20 ug/Lane) Lane 10: NIH/3T3 cell lysate (20 ug/Lane) Lane 11: C6 cell lysate (20 ug/Lane) Lane 12: Mouse kidney tissue lysate (40 ug/Lane) Lane 13: Mouse liver tissue lysate (40 ug/Lane) Lane 14: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33132) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33132_mouse-nm23-h1-mab-13c2-r-28820241423318.jpg "Western Blot: NM23-H1 Antibody (13C2-R) [NBP3-33132] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: Raji cell lysate (20 ug/Lane) Lane 5: HeLa cell lysate (20 ug/Lane) Lane 6: K-562 cell lysate (20 ug/Lane) Lane 7: A549 cell lysate (20 ug/Lane) Lane 8: A431 cell lysate (20 ug/Lane) Lane 9: HepG2 cell lysate (20 ug/Lane) Lane 10: NIH/3T3 cell lysate (20 ug/Lane) Lane 11: C6 cell lysate (20 ug/Lane) Lane 12: Mouse kidney tissue lysate (40 ug/Lane) Lane 13: Mouse liver tissue lysate (40 ug/Lane) Lane 14: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33132) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NM23-H1 Antibody (13C2-R) [NBP3-33132] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33132) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33132_mouse-nm23-h1-mab-13c2-r-29820241492692.jpg)
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (13C2-R) [NBP3-33132] - Immunocytochemistry analysis of HeLa cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33132_mouse-nm23-h1-mab-13c2-r-2920241192324.jpg)
![Western Blot: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Western-Blot-NBP2-89736-img0002.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Analysis of Human AGT expression on HepG2 cells. The cells were treated ,stained with purified anti-Human AGT, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Flow-Cytometry-NBP2-89736-img0004.jpg)
![Immunoprecipitation: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-SerpinA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SerpinA8 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa Observed band size: 51 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Immunoprecipitation-NBP2-89736-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Staining of Human AGT in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89736 antibody (1:60). Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89736_rabbit-monoclonal-serpin-a8-angiotensinogen-antibody-001-23520251129370.png)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Western blot analysis of Sphingosine Kinase 1/SPHK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Western-Blot-NBP2-67164-img0005.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Western blot analysis of Sphingosine Kinase 1/SPHK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate")
![Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of paraffin-embedded human kidney tissue using anti-SPHK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Immunohistochemistry-Paraffin-NBP2-67164-img0002.jpg)
![Flow Cytometry: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of Raji cells with SPHK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Flow-Cytometry-NBP2-67164-img0001.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of SPHK1 on C2C12 lysates using anti-SPHK1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Western-Blot-NBP2-67164-img0004.jpg)
![Western Blot: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Western blot analysis of SEC14L2 on different lysates with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 16 seconds. 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32935) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-288202412442940.jpg "Western Blot: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Western blot analysis of SEC14L2 on different lysates with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 16 seconds. 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32935) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-298202414262627.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Immunocytochemistry analysis of A549 cells labeling SEC14L2 with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-29202410234211.jpg)
![Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324] - Immunochemical staining of human ECH1 in human prostate with NBP3-00324 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00324_rabbit-polyclonal-ech1-antibody-23520252135181.png "Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324] - Immunochemical staining of human ECH1 in human prostate with NBP3-00324 (1:10000, formalin-fixed paraffin embedded sections).")
![Western Blot: ECH1 Antibody [NBP3-00324] - NBP3-00324 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:36 kDa Observed band size:34 kDa](http://images.novusbio.com/fullsize/nbp3-00324_rabbit-polyclonal-ech1-antibody-235202514371321.png)
![Immunoprecipitation: ECH1 Antibody [NBP3-00324] - ECH1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00324 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00324, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 34 kDa Observed band size: 34 kDa](http://images.novusbio.com/fullsize/nbp3-00324_rabbit-polyclonal-ech1-antibody-23520251129318.png)
![Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324] - Immunochemical staining of human ECH1 in human liver(cirrhosis) with NBP3-00324 (1:10000, formalin-fixed paraffin embedded sections).Positive staining was localized to cytoplasm of hepatocyte](http://images.novusbio.com/fullsize/nbp3-00324_rabbit-polyclonal-ech1-antibody-23520252152502.png)