| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89736-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89830-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54501-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26427-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47180SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26636-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47179SS | |||
![Western Blot: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Western-Blot-NBP2-89736-img0002.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Analysis of Human AGT expression on HepG2 cells. The cells were treated ,stained with purified anti-Human AGT, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Flow-Cytometry-NBP2-89736-img0004.jpg)
![Immunoprecipitation: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-SerpinA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SerpinA8 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa Observed band size: 51 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Immunoprecipitation-NBP2-89736-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Staining of Human AGT in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89736 antibody (1:60). Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89736_rabbit-monoclonal-serpin-a8-angiotensinogen-antibody-001-23520251129370.png)
![Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Anti-CD39L4/ENTPD5 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 53 kDa](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-064-Western-Blot-NBP2-89830-img0001.jpg "Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Anti-CD39L4/ENTPD5 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 53 kDa")
![Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Immunochemical staining of human CD39L4/ENTPD5 in human liver with NBP2-89830 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-89830_rabbit-monoclonal-cd39l4-entpd5-antibody-064-235202511545677.png)
![Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Immunochemical staining of human CD39L4/ENTPD5 in human kidney with NBP2-89830 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-89830_rabbit-monoclonal-cd39l4-entpd5-antibody-064-235202511444489.png)
![Immunoprecipitation: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - ENTPD5 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 0.5 uL NBP2-89830 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP2-89830 at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 47 kDa](http://images.novusbio.com/fullsize/nbp2-89830_rabbit-monoclonal-cd39l4-entpd5-antibody-064-23520251154565.png)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Western blot analysis of Pyruvate Dehydrogenase E1-alpha subunit on different lysates with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HEK-293 cell lysate (20 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32787) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-288202412442923.jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Western blot analysis of Pyruvate Dehydrogenase E1-alpha subunit on different lysates with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HEK-293 cell lysate (20 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32787) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32787) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-298202414115418.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Immunocytochemistry analysis of Jurkat cells treated with or without lambda pp labeling Pyruvate Dehydrogenase E1-alpha subunit with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-2920241114815.jpg)
![Western Blot: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - Novus Biologicals antibody NB300-109 was used to detect the protein on a Western blot. 150 ng of protein were added to the Western blot.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Protein-Western-Blot-NBP1-42461-img0002.jpg "Western Blot: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - Novus Biologicals antibody NB300-109 was used to detect the protein on a Western blot. 150 ng of protein were added to the Western blot.")
![SDS-Page: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - This product has a purity >95%. This protein was run on a 4-20% SDS-PAGE gel with 5 ug protein loaded.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Protein-Immunoblotting-NBP1-42461-img0001.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Western Blot analysis of Kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/1723).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Azide-and-BSA-Free-Western-Blot-NBP2-54501-img0004.jpg "Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Western Blot analysis of Kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/1723).")
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma Stained with AMACR / p504S Monoclonal Antibody (AMACR/1723)](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54501-img0001.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Western Blot Analysis of PC3 cell lysate using alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Azide-and-BSA-Free-Western-Blot-NBP2-54501-img0002.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Western Blot Analysis of Liver tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Azide-and-BSA-Free-Western-Blot-NBP2-54501-img0003.jpg)
![Western Blot: NM23-H1 Antibody [NBP2-19549] - Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Western-Blot-NBP2-19549-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NM23-H1 Antibody [NBP2-19549] - Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody [NBP2-19549] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/nm23-H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19549-img0003.jpg)
![Western Blot: NM23-H1 Antibody [NBP2-19549] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/nm23-H1-Antibody-Western-Blot-NBP2-19549-img0001.jpg)
![Western Blot: NM23-H1 Antibody [NBP2-19549] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/nm23-H1-Antibody-Western-Blot-NBP2-19549-img0002.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Western Blot analysis of Kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/1723).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Western-Blot-NBP2-53390-img0004.jpg "Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Western Blot analysis of Kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/1723).")
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma Stained with AMACR / p504S Monoclonal Antibody (AMACR/1723)](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Immunohistochemistry-Paraffin-NBP2-53390-img0001.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Western Blot Analysis of PC3 cell lysate using alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Western-Blot-NBP2-53390-img0002.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Western Blot Analysis of Liver tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-AMACR-1723-Western-Blot-NBP2-53390-img0003.jpg)
![Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-262202414471571.jpg "Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-262202415475635.jpg)
![Western Blot: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Positive Western Blot detected in Hela whole cell lysate (treated with Calyculin A or EGF). All lanes: eIF2 alpha/EIF2S1 [p Ser51] Antibody at 1.48ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36 KDa Observed band size: 36 KDa](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-282202411442641.jpg)
![Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Staining of A549 cells (treated with 100ng/ml EGF for 20min) with eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-282202412493624.jpg)
![Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-12-Antibody-exon-12-Western-Blot-NBP1-47180-img0003.jpg "Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.")
![Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - NBP3-26636 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101148.jpg "Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - NBP3-26636 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody")
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of Hela cell with NBP3-26636 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101147.jpg)
![Western Blot: Aromatase Antibody (1H1) [NBP3-26636] - Positive WB detected in: 293 whole cell lysate (20µg), K562 whole cell lysate (20µg), Hela whole cell lysate (20µg), MCF-7 whole cell lysate (20µg), PC-3 whole cell lysate (20µg)All lanes: CYP19A1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 kDaObserved band size: 58 kDaExposure time：120s](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101143.jpg)
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-26120251011416.jpg)
![Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-10-Antibody-exon-10-Western-Blot-NBP1-47179-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein")
![Western Blot: BMAL1 Antibody [NBP2-15599] - Analysis of ARNTL expression in transfected 293T cell line by ARNTL polyclonal antibody. A: Non-transfected lysate. B: ARNTL transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Western-Blot-NBP2-15599-img0005.jpg "Western Blot: BMAL1 Antibody [NBP2-15599] - Analysis of ARNTL expression in transfected 293T cell line by ARNTL polyclonal antibody. A: Non-transfected lysate. B: ARNTL transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody [NBP2-15599] - A375 cells. Green: MOP3 protein stained by MOP3 antibody [N1N3] Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15599-img0003.jpg)
![Western Blot: BMAL1 Antibody [NBP2-15599] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Western-Blot-NBP2-15599-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: BMAL1 Antibody [NBP2-15599] - Treated 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] and PER2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15599_rabbit-polyclonal-bmal1-antibody-1610202419405778.jpg)