| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22033-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89486-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83300-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95253-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31925-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80951-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03522-20ul | |||
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - Western blot analysis using NBP3-22033 in A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-57202317215160.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - Western blot analysis using NBP3-22033 in A549 cell lysate.")
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: MCF-7. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202414462214.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: SH-SY5Y. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-2342024155511.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: Hep-G2. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202414462212.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: Mouse lung. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202415232515.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: Rat kidney. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202414462210.jpg)
![Immunocytochemistry/Immunofluorescence: NIT2 Antibody [NBP1-89486] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & centrosome.](http://images.novusbio.com/fullsize/NIT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89486-img0007.jpg "Immunocytochemistry/Immunofluorescence: NIT2 Antibody [NBP1-89486] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & centrosome.")
![Immunohistochemistry-Paraffin: NIT2 Antibody [NBP1-89486] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/NIT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89486-img0001.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human plasma (IgG/HSA depleted)](http://images.novusbio.com/fullsize/nbp1-89486_rabbit-polyclonal-nit2-antibody-15520258544710.jpg)
![Immunohistochemistry-Paraffin: HADH Antibody [NBP1-83300] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/HADH-Antibody-Immunohistochemistry-Paraffin-NBP1-83300-img0002.jpg "Immunohistochemistry-Paraffin: HADH Antibody [NBP1-83300] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.")
![Western Blot: IDH2 Antibody [NBP1-52117] - 0.1ug/ml staining of Mouse (A), Rat (B) and Pig (C) Heart lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP1-52117-img0003.jpg "Western Blot: IDH2 Antibody [NBP1-52117] - 0.1ug/ml staining of Mouse (A), Rat (B) and Pig (C) Heart lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-52117] - 3.75ug/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-52117-img0002.jpg)
![Western Blot: IDH2 Antibody [NBP1-52117] - Analysis of IDH2 in Human Heart lysate (35ug protein in RIPA buffer) using NBP1-52117 at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP1-52117-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence;IDH2 Antibody[NBP3-29974]Detection of human IDH2 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-IDH2 used at a dilution of 1:1,000 . Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/nbp3-29996_rabbit-idh2-pab-78202410372610.jpg "Immunocytochemistry/ Immunofluorescence;IDH2 Antibody[NBP3-29974]Detection of human IDH2 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-IDH2 used at a dilution of 1:1,000 . Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.")
![Immunohistochemistry;IDH2 Antibody[NBP3-29975]Detection of mouse IDH2 by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-IDH2 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29996_rabbit-idh2-pab-7820241039269.jpg)
![Immunohistochemistry;IDH2 Antibody[NBP3-29996]Detection of human IDH2 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma and lung cancer . Antibody: Affinity purified rabbit anti-IDH2 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29996_rabbit-idh2-pab-782024936138.jpg)
![Immunoprecipitation:IDH2 AntibodyNBP3-29996] -Detection of human IDH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-IDH2 antibody NBP3-29996 (lot NBP3-29996-1) used for IP at 6 µg/mg lysate. For blotting immunoprecipitated IDH2, NBP3-29996 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29996_rabbit-idh2-pab-3012202419525811.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - analysis of various lysates, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.5s.](http://images.novusbio.com/fullsize/nbp2-95253_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-western-blot-241202412935..jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - analysis of various lysates, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.5s.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] -Analysis of paraffin-embedded rat kidney using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp2-95253_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-2412024121118..jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - Human thyroid cancer using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp2-95253_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-2412024121229..jpg)
![Western Blot: IDH2 Antibody (8C3D4) [NBP3-15366] - Western blot analysis of extracts of various cell lines, using IDH2 Rabbit mAb (NBP3-15366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/IDH2-Antibody-8C3D4-Western-Blot-NBP3-15366-img0004.jpg "Western Blot: IDH2 Antibody (8C3D4) [NBP3-15366] - Western blot analysis of extracts of various cell lines, using IDH2 Rabbit mAb (NBP3-15366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody (8C3D4) [NBP3-15366] - Immunofluorescence analysis of NIH-3T3 cells using IDH2 Rabbit mAb (NBP3-15366) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IDH2-Antibody-8C3D4-Immunocytochemistry-Immunofluorescence-NBP3-15366-img0003.jpg)
![Immunohistochemistry-Paraffin: IDH2 Antibody (8C3D4) [NBP3-15366] - Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH2 Rabbit mAb (NBP3-15366) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IDH2-Antibody-8C3D4-Immunohistochemistry-Paraffin-NBP3-15366-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody (8C3D4) [NBP3-15366] - Immunofluorescence analysis of C6 cells using IDH2 Rabbit mAb (NBP3-15366) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IDH2-Antibody-8C3D4-Immunocytochemistry-Immunofluorescence-NBP3-15366-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31925-img0010.jpg "Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining in human testis and kidney tissues using anti-GAPDHS antibody. Corresponding GAPDHS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0009.jpg)
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0007.jpg)
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ACOX1 Antibody [NBP1-80951] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACOX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80951-img0005.jpg "Immunocytochemistry/Immunofluorescence: ACOX1 Antibody [NBP1-80951] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: ACOX1 Antibody [NBP1-80951] - Staining of human pancreas shows weak cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/ACOX1-Antibody-Immunohistochemistry-Paraffin-NBP1-80951-img0011.jpg)
![Immunohistochemistry-Paraffin: ACOX1 Antibody [NBP1-80951] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACOX1-Antibody-Immunohistochemistry-Paraffin-NBP1-80951-img0008.jpg)
![Immunohistochemistry-Paraffin: ACOX1 Antibody [NBP1-80951] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ACOX1-Antibody-Immunohistochemistry-Paraffin-NBP1-80951-img0009.jpg)
![Immunohistochemistry-Paraffin: ACOX1 Antibody [NBP1-80951] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACOX1-Antibody-Immunohistochemistry-Paraffin-NBP1-80951-img0010.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of human breast cells lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NB100-1940-img0002.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of human breast cells lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of paraffin embedded human small intestine. Antibody at 5 ug/mL.Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunohistochemistry-Paraffin-NB100-1940-img0003.jpg)
![Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Western-Blot-NBP3-03522-img0001.jpg "Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Rat heart using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-52202516334192.jpg)
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded human breast using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-52202516293948.jpg)
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-5220251628067.jpg)
![Western Blot: HADH Antibody (D10-E7-R) [NBP3-32416] - Western blot analysis of HADH on different lysates with Mouse anti-HADH antibody (NBP3-32416) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Lane 10: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-278202415295516.jpg "Western Blot: HADH Antibody (D10-E7-R) [NBP3-32416] - Western blot analysis of HADH on different lysates with Mouse anti-HADH antibody (NBP3-32416) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Lane 10: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HADH Antibody (D10-E7-R) [NBP3-32416] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-HADH antibody (NBP3-32416) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32416) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-29820241420931.jpg)
![Immunocytochemistry/ Immunofluorescence: HADH Antibody (D10-E7-R) [NBP3-32416] - Immunocytochemistry analysis of HeLa cells labeling HADH with Mouse anti-HADH antibody (NBP3-32416) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HADH antibody (NBP3-32416) at 1/150 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-29202410334032.jpg)