| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70875 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-151SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13891-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90233-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92324-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89545-25ul | |||
![Western Blot: thioredoxin reductase 1 Antibody [NBP2-20620] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/thioredoxin-reductase-1-Antibody-Western-Blot-NBP2-20620-img0001.jpg "Western Blot: thioredoxin reductase 1 Antibody [NBP2-20620] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Thioredoxin Reductase 1/TRXR1 Antibody [NBP2-20620] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/thioredoxin-reductase-1-Antibody-Immunohistochemistry-Paraffin-NBP2-20620-img0004.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Reductase 1/TRXR1 Antibody [NBP2-20620] - Analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/thioredoxin-reductase-1-Antibody-Immunohistochemistry-Paraffin-NBP2-20620-img0002.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Reductase 1/TRXR1 Antibody [NBP2-20620] - Analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/thioredoxin-reductase-1-Antibody-Immunohistochemistry-Paraffin-NBP2-20620-img0003.jpg)
![Western Blot: HADH Antibody (3D12) [NBP2-45931] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HADH.](http://images.novusbio.com/fullsize/HADH-Antibody-3D12-Western-Blot-NBP2-45931-img0006.jpg "Western Blot: HADH Antibody (3D12) [NBP2-45931] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HADH.")
![Immunohistochemistry: HADH Antibody (3D12) [NBP2-45931] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-3D12-Immunohistochemistry-NBP2-45931-img0005.jpg)
![Western Blot: HADH Antibody (3D12) [NBP2-45931] - Analysis of HT29 cell lysate (35ug) by using HADH monoclonal antibody.](http://images.novusbio.com/fullsize/HADH-Antibody-3D12-Western-Blot-NBP2-45931-img0007.jpg)
![Immunohistochemistry: HADH Antibody (3D12) [NBP2-45931] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-3D12-Immunohistochemistry-NBP2-45931-img0001.jpg)
![Immunohistochemistry: HADH Antibody (3D12) [NBP2-45931] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-3D12-Immunohistochemistry-NBP2-45931-img0002.jpg)
![Immunohistochemistry: HADH Antibody (3D12) [NBP2-45931] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-3D12-Immunohistochemistry-NBP2-45931-img0003.jpg)
![Immunohistochemistry: HADH Antibody (3D12) [NBP2-45931] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-3D12-Immunohistochemistry-NBP2-45931-img0004.jpg)
![Western Blot: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of HT29 cell lysate (35ug) by using HADH monoclonal antibody.](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Western-Blot-NBP2-70875-img0002.jpg "Western Blot: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of HT29 cell lysate (35ug) by using HADH monoclonal antibody.")
![Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70875-img0006.jpg)
![Western Blot: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HADH.](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Western-Blot-NBP2-70875-img0001.jpg)
![Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70875-img0003.jpg)
![Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70875-img0004.jpg)
![Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70875-img0005.jpg)
![Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70875-img0007.jpg)
![Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2682024158262.jpg "Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds")
![Western Blot: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: Huh7 cell lysate Lane 5: Hela cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32722) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-27820241631640.jpg)
![Immunohistochemistry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32722) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29820241492649.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunocytochemistry analysis of HepG2 cells labeling Pyruvate Carboxylase with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2920241023429.jpg)
![Flow Cytometry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Flow cytometric analysis of PC-12 cells labeling Pyruvate Carboxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32722, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29202416372196.jpg)
![Immunohistochemistry-Paraffin: CYP11B2 Antibody [NBP2-13891] - Analysis in human adrenal gland and tonsil tissues using NBP2-13891 antibody. Corresponding CYP11B2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Immunohistochemistry-Paraffin-NBP2-13891-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CYP11B2 Antibody [NBP2-13891] - Analysis in human adrenal gland and tonsil tissues using NBP2-13891 antibody. Corresponding CYP11B2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CYP11B2 Antibody [NBP2-13891] - Staining of human Liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Immunohistochemistry-Paraffin-NBP2-13891-img0010.jpg)
![Immunohistochemistry-Paraffin: CYP11B2 Antibody [NBP2-13891] - Staining of human Adrenal gland shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Immunohistochemistry-Paraffin-NBP2-13891-img0008.jpg)
![Immunohistochemistry-Paraffin: CYP11B2 Antibody [NBP2-13891] - Staining of human Endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Immunohistochemistry-Paraffin-NBP2-13891-img0009.jpg)
![Immunohistochemistry-Paraffin: CYP11B2 Antibody [NBP2-13891] - Staining of human Tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Immunohistochemistry-Paraffin-NBP2-13891-img0011.jpg)
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in NIH/3T3.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0011.jpg "Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in NIH/3T3.")
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4 Analysis of NADK expression in HeLa.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0008.jpg)
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in HepG2.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0009.jpg)
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in Raw 264.7.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0010.jpg)
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - Analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody (M01), clone 5F4.Lane 1: NADK transfected lysate(49.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0012.jpg)
![ELISA: NADK Antibody (5F4) [H00065220-M01] - Detection limit for recombinant GST tagged NADK is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-ELISA-H00065220-M01-img0013.jpg)
![Western Blot: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of extracts of various cell lines, using FTO Rabbit mAb (NBP3-16285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Western-Blot-NBP3-16285-img0003.jpg "Western Blot: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of extracts of various cell lines, using FTO Rabbit mAb (NBP3-16285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: FTO Antibody (2Q9K10) [NBP3-16285] - Immunofluorescence analysis of U-2 OS cells using FTO Rabbit mAb (NBP3-16285) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Immunocytochemistry-Immunofluorescence-NBP3-16285-img0002.jpg)
![Immunohistochemistry-Paraffin: FTO Antibody (2Q9K10) [NBP3-16285] - Human bladder cancer using FTO Rabbit mAb (NBP3-16285) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Immunohistochemistry-Paraffin-NBP3-16285-img0001.jpg)
![Immunoprecipitation: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of 300ug extracts of 293T cells using 3ug FTO antibody (NBP3-16285). Western blot was performed from the immunoprecipitate using FTO antibody (NBP3-16285) at a dilition of 1:500.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Immunoprecipitation-NBP3-16285-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: FTO Antibody (2Q9K10) [NBP3-16285] - Confocal imaging of U-2 OS cells using FTO Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16285_rabbit-fto-mab-2q9k10-52202511383732.jpg)
![Western Blot: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: PC-12 cell lysate Lane 4: mouse lymph node tissue Lane 5: rat liver tissue Lane 6: rat brain tissue Lysates/proteins at 30 ug/Lane. Predicted band size: 129.6 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32723) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-278202415295530.jpg "Western Blot: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: PC-12 cell lysate Lane 4: mouse lymph node tissue Lane 5: rat liver tissue Lane 6: rat brain tissue Lysates/proteins at 30 ug/Lane. Predicted band size: 129.6 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32723) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32723) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-29820241420961.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Immunocytochemistry analysis of PC-12 cells labeling PBC with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-29202410334022.jpg)
![Flow Cytometry: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Flow cytometric analysis of PC-12 cells labeling Pyruvate Carboxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32723, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-392024884671.jpg)
![Immunocytochemistry/Immunofluorescence: RDH13 Antibody [NBP1-92324] - Staining of human cell line A-431 shows localization to cytosol & mitochondria.](http://images.novusbio.com/fullsize/RDH13-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92324-img0005.jpg "Immunocytochemistry/Immunofluorescence: RDH13 Antibody [NBP1-92324] - Staining of human cell line A-431 shows localization to cytosol & mitochondria.")
![Immunohistochemistry-Paraffin: RDH13 Antibody [NBP1-92324] - Staining of human retina shows cytoplasmic positivity in photoreceptor inner segments and nerve fibers.](http://images.novusbio.com/fullsize/RDH13-Antibody-Immunohistochemistry-Paraffin-NBP1-92324-img0006.jpg)
![Immunohistochemistry-Paraffin: RDH13 Antibody [NBP1-92324] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/RDH13-Antibody-Immunohistochemistry-Paraffin-NBP1-92324-img0002.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-92324_rabbit-polyclonal-rdh13-antibody-295202516275524.jpg)
![Immunocytochemistry/Immunofluorescence: ETFB Antibody [NBP1-89545] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria & vesicles.](http://images.novusbio.com/fullsize/ETFB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89545-img0009.jpg "Immunocytochemistry/Immunofluorescence: ETFB Antibody [NBP1-89545] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria & vesicles.")
![Immunohistochemistry-Paraffin: ETFB Antibody [NBP1-89545] - Staining of human heart muscle shows cytoplasmic positivity with a granular pattern in myocytes.](http://images.novusbio.com/fullsize/ETFB-Antibody-Immunohistochemistry-Paraffin-NBP1-89545-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ETFB Antibody [NBP1-89545] - Analysis using Anti-ETFB antibody NBP1-89545 (A) shows similar pattern to independent antibody NBP1-86040 (B).](http://images.novusbio.com/fullsize/ETFB-Antibody-Western-Blot-NBP1-89545-img0011.jpg)
