| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06239-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89915-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB41442-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87485-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82048-0.025ml | |||
![Western Blot: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: PC-12 cell lysate Lane 4: mouse lymph node tissue Lane 5: rat liver tissue Lane 6: rat brain tissue Lysates/proteins at 30 ug/Lane. Predicted band size: 129.6 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32723) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-278202415295530.jpg "Western Blot: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: PC-12 cell lysate Lane 4: mouse lymph node tissue Lane 5: rat liver tissue Lane 6: rat brain tissue Lysates/proteins at 30 ug/Lane. Predicted band size: 129.6 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32723) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32723) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-29820241420961.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Immunocytochemistry analysis of PC-12 cells labeling PBC with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-29202410334022.jpg)
![Flow Cytometry: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Flow cytometric analysis of PC-12 cells labeling Pyruvate Carboxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32723, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-392024884671.jpg)
![Western Blot: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Western blot analysis of PLC-gamma 2 on different lysates with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 148 kDa Observed band size: 148 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32827) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-288202412461651.jpg "Western Blot: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Western blot analysis of PLC-gamma 2 on different lysates with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 148 kDa Observed band size: 148 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32827) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32827) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-298202414333622.jpg)
![Immunocytochemistry/ Immunofluorescence: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Immunocytochemistry analysis of Raji cells labeling PLC-gamma 2 with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-29202410334047.jpg)
![Flow Cytometry: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Flow cytometric analysis of Raji cells labeling PLC-gamma 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32827, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-392024884627.jpg)
![Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-278202416285288.jpg "Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CLPP Antibody (JE40-00) [NBP3-32195] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29820241492667.jpg)
![Immunocytochemistry/ Immunofluorescence: CLPP Antibody (JE40-00) [NBP3-32195] - Immunocytochemistry analysis of HeLa cells labeling CLPP with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202410334080.jpg)
![Flow Cytometry: CLPP Antibody (JE40-00) [NBP3-32195] - Flow cytometric analysis of HeLa cells labeling CLPP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32195, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202416372131.jpg)
![Western Blot: CLPP Antibody [NBP3-06239] - Anti-CLPP rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: K562. Whole Cell LysateLane D: A549 Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NBP3-06239-img0003.jpg "Western Blot: CLPP Antibody [NBP3-06239] - Anti-CLPP rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: K562. Whole Cell LysateLane D: A549 Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa")
![Immunoprecipitation: CLPP Antibody [NBP3-06239] -](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunoprecipitation-NBP3-06239-img0002.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody - BSA Free [NBP3-06239] - Immunochemical staining of human CLPP in human liver with NBP3-06239 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-23520251433153.png)
![Immunohistochemistry-Paraffin: CLPP Antibody - BSA Free [NBP3-06239] - Immunochemical staining of human CLPP in human pancreas with NBP3-06239 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-2352025112932.png)
![Immunocytochemistry/Immunofluorescence: CLPP Antibody [NBP3-06239] - Immunofluorescence staining of CLPP in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06239 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-23520251633504.png)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:40 kDa](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-034-Western-Blot-NBP2-89915-img0003.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:40 kDa")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of human ARG1 in human kidney with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-034-Immunohistochemistry-Paraffin-NBP2-89915-img0002.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of human ARG1 in human liver with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-034-Immunohistochemistry-Paraffin-NBP2-89915-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of ARG1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89915 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89915_rabbit-monoclonal-arginase-1-arg1-liver-arginase-antibody-034-23520252135123.png)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Analysis of Human ARG1 expression on HepG2 cells. The cells were treated, stained with NBP2-89915, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/nbp2-89915_rabbit-monoclonal-arginase-1-arg1-liver-arginase-antibody-034-235202511545610.png)
![Western Blot: CD38 Antibody (PD01-49) [NBP3-32151] - Western blot analysis of CD38 on Daudi cell lysates with Rabbit anti-CD38 antibody (NBP3-32151) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 34 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32151) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-278202416285274.jpg "Western Blot: CD38 Antibody (PD01-49) [NBP3-32151] - Western blot analysis of CD38 on Daudi cell lysates with Rabbit anti-CD38 antibody (NBP3-32151) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 34 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32151) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD38 Antibody (PD01-49) [NBP3-32151] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD38 antibody (NBP3-32151) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32151) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-29820241435231.jpg)
![Immunocytochemistry/ Immunofluorescence: CD38 Antibody (PD01-49) [NBP3-32151] - Immunocytochemistry analysis of A549 cells labeling CD38 with Rabbit anti-CD38 antibody (NBP3-32151) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD38 antibody (NBP3-32151) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-2920241192330.jpg)
![Flow Cytometry: CD38 Antibody (PD01-49) [NBP3-32151] - Flow cytometric analysis of THP-1 cells labeling CD38. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32151, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-29202416453252.jpg)
![Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-288202412461658.jpg "Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32834) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-298202414333688.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunocytochemistry analysis of HepG2 cells labeling PMP70 with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-29202410334056.jpg)
![Flow Cytometry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Flow cytometric analysis of NIH/3T3 cells labeling PMP70. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32834, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-2920241637212.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining in human pancreas and liver tissues using anti-INS antibody. Corresponding INS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Insulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87485-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining in human pancreas and liver tissues using anti-INS antibody. Corresponding INS RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Insulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87485-img0008.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining of human pancreas shows high expression.](http://images.novusbio.com/fullsize/Insulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87485-img0009.jpg)
![Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP) NB100-82048](http://images.novusbio.com/fullsize/MKK3-MEK3-[p-Ser189]-Antibody-Western-Blot-NB100-82048-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP) NB100-82048")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Staining of methanol-fixed Hela cells using NB100-82048](http://images.novusbio.com/fullsize/MKK3-MEK3-[p-Ser189]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82048-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extracts from Hela cells untreated or treated with PMA using MKK3(Phospho-Ser189) antibody](http://images.novusbio.com/fullsize/MKK3-MEK3-[p-Ser189]-Antibody-Western-Blot-NB100-82048-img0007.jpg)
![Immunohistochemistry-Paraffin: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using NB100-82048(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-82048_rabbit-polyclonal-mkk3-mek3-p-ser189-antibody-103202523464532.jpg)
![Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of MAP2K3 expression in transfected 293T cell line by MAP2K3 monoclonal antibody (M02), clone 1D10.Lane 1: MAP2K3 transfected lysate (Predicted MW: 39.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-1D10-Western-Blot-H00005606-M02-img0010.jpg "Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of MAP2K3 expression in transfected 293T cell line by MAP2K3 monoclonal antibody (M02), clone 1D10.Lane 1: MAP2K3 transfected lysate (Predicted MW: 39.3 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of monoclonal antibody to MAP2K3 on formalin-fixed paraffin-embedded human dysgerminoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-1D10-Immunohistochemistry-Paraffin-H00005606-M02-img0007.jpg)
![Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Western Blot analysis of MAP2K3 expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-1D10-Western-Blot-H00005606-M02-img0001.jpg)
![Immunoprecipitation: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of MAP2K3 transfected lysate using anti-MAP2K3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAP2K3 monoclonal antibody.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-1D10-Immunoprecipitation-H00005606-M02-img0008.jpg)
![Sandwich ELISA: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Detection limit for recombinant GST tagged MAP2K3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-1D10-Sandwich-ELISA-H00005606-M02-img0009.jpg)
![Western Blot: CD98 Antibody [H00006520-D01P] - Analysis of SLC3A2 expression in rat brain.](http://images.novusbio.com/fullsize/CD98-Antibody-Western-Blot-H00006520-D01P-img0009.jpg "Western Blot: CD98 Antibody [H00006520-D01P] - Analysis of SLC3A2 expression in rat brain.")
![Western Blot: CD98 Antibody [H00006520-D01P] - Analysis of SLC3A2 expression in A-431.](http://images.novusbio.com/fullsize/CD98-Antibody-Western-Blot-H00006520-D01P-img0006.jpg)
![Western Blot: CD98 Antibody [H00006520-D01P] - Analysis of SLC3A2 expression in mouse kidney.](http://images.novusbio.com/fullsize/CD98-Antibody-Western-Blot-H00006520-D01P-img0007.jpg)
![Western Blot: CD98 Antibody [H00006520-D01P] - Analysis of SLC3A2 expression in mouse brain.](http://images.novusbio.com/fullsize/CD98-Antibody-Western-Blot-H00006520-D01P-img0008.jpg)
![Western Blot: CD98 Antibody [H00006520-D01P] - Analysis of SLC3A2 expression in transfected 293T cell line by SLC3A2 polyclonal antibody.Lane 1: SLC3A2 transfected lysate(57.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CD98-Antibody-Western-Blot-H00006520-D01P-img0010.jpg)