| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89705-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81083-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81084-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81055-0.025mg | |||
![Western Blot: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705] - Lane A: NCI-H1299 Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:63 kDa Observed band size:70 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-001-Western-Blot-NBP2-89705-img0002.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705] - Lane A: NCI-H1299 Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:63 kDa Observed band size:70 kDa (We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705] - Staining of human NT5E in human spleen with rabbit monoclonal antibody (1:5000).](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89705-img0001.jpg)
![Western Blot: S100B Antibody (PD00-11) [NBP3-32924] - Western blot analysis of S100B on SK-MEL-28 cell lysates with Rabbit anti-S100B antibody (NBP3-32924) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 1 minute; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32924) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32924_mouse-s100b-mab-pd00-11-288202412442930.jpg "Western Blot: S100B Antibody (PD00-11) [NBP3-32924] - Western blot analysis of S100B on SK-MEL-28 cell lysates with Rabbit anti-S100B antibody (NBP3-32924) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 1 minute; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32924) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: S100B Antibody (PD00-11) [NBP3-32924] - Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Mouse anti-S100B antibody (NBP3-32924) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32924) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32924_mouse-s100b-mab-pd00-11-298202414115432.jpg)
![Western Blot: PLC-beta 3 Antibody (JE39-14) [NBP3-32776] - Western blot analysis of PLC-beta 3 on different lysates with Rabbit anti-PLC-beta 3 antibody (NBP3-32776) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: HCT 116 cell lysate Lane 5: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 139 kDa Observed band size: 150 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32776) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32776_rabbit-plc-beta-3-mab-je39-14-288202412442979.jpg "Western Blot: PLC-beta 3 Antibody (JE39-14) [NBP3-32776] - Western blot analysis of PLC-beta 3 on different lysates with Rabbit anti-PLC-beta 3 antibody (NBP3-32776) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: HCT 116 cell lysate Lane 5: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 139 kDa Observed band size: 150 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32776) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PLC-beta 3 Antibody (JE39-14) [NBP3-32776] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-PLC-beta 3 antibody (NBP3-32776) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32776) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32776_rabbit-plc-beta-3-mab-je39-14-298202414262641.jpg)
![Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Separation of human CD268 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/BAFFR-TNFRSF13C-Antibody-11C1-Flow-Cytometry-NBP3-09142-img0002.jpg "Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Separation of human CD268 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).")
![Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Surface staining pattern of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/BAFFR-TNFRSF13C-Antibody-11C1-Flow-Cytometry-NBP3-09142-img0001.jpg)
![Western Blot: AIF-1/Iba1 Antibody (2A2-B6) [H00000199-M01] - Analysis of AIF1 expression in K-562 using AIF-1/Iba1 Antibody (2A2-B6).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-2A2-B6-Western-Blot-H00000199-M01-img0005.jpg "Western Blot: AIF-1/Iba1 Antibody (2A2-B6) [H00000199-M01] - Analysis of AIF1 expression in K-562 using AIF-1/Iba1 Antibody (2A2-B6).")
![ELISA: AIF-1/Iba1 Antibody (2A2-B6) [H00000199-M01] - Detection limit for recombinant GST tagged AIF-1:Iba1 is 0.03 ng/ml using AIF-1:Iba1 Antibody (2A2-B6) as a capture antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-2A2-B6-ELISA-H00000199-M01-img0004.jpg)
![Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083] - Flow-cytometry using the L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083]. Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 (NBP2-81083, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-MEL-14-Flow-Cytometry-NBP2-81083-img0001.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083] - Flow-cytometry using the L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083]. Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 (NBP2-81083, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) - Chimeric - Azide and BSA Free [NBP2-81083] - Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or NBP2-81083 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp2-81083_rabbit-monoclonal-l-selectin-cd62l-antibody-mel-14-74202522141216.png)
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG-56) - Chimeric [NBP2-81084] - Flow-cytometry using [NBP2-81084]. Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of DREG-56 (NBP2-81084, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG-56-Chimeric-Flow-Cytometry-NBP2-81084-img0001.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (DREG-56) - Chimeric [NBP2-81084] - Flow-cytometry using [NBP2-81084]. Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of DREG-56 (NBP2-81084, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: CD38 Antibody (AT13/5) [NBP2-81055] - Immunofluorescence staining of fixed Daudi cells with anti-CD38 antibody AT13/5 [NBP2-81055]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of AT13/5 [NBP2-81055] at 10 ug/mL for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81055, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-AT13-5-Immunocytochemistry-Immunofluorescence-NBP2-81055-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD38 Antibody (AT13/5) [NBP2-81055] - Immunofluorescence staining of fixed Daudi cells with anti-CD38 antibody AT13/5 [NBP2-81055]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of AT13/5 [NBP2-81055] at 10 ug/mL for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81055, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: CD38 Antibody (AT13/5) [NBP2-81055] - Western Blot using anti-CD38 antibody (AT13/5) [NBP2-81055]. Human spleen lysate samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of AT13/5 [NBP2-81055] at 1 ug/mL before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD38 is 34.3 kDa, but this protein is glycosylated at several residues. NBP2-81055 successfully detected CD38 in human spleen lysate.](http://images.novusbio.com/fullsize/CD38-Antibody-AT13-5-Western-Blot-NBP2-81055-img0001.jpg)
![Western Blot: MEKK1 Antibody [NBP2-89023] - Western Blot analysis using MEKK1, pAb](http://images.novusbio.com/fullsize/MEKK1-Antibody-Western-Blot-NBP2-89023-img0001.jpg "Western Blot: MEKK1 Antibody [NBP2-89023] - Western Blot analysis using MEKK1, pAb")