| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-07075SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95194-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37249-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06497-50ul | |||
![Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (2597C) [NBP3-07075] - HIF-2 alpha /EPAS1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Human HIF-2 alpha /EPAS1 Monoclonal Antibody (Catalog # NBP3-07075) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-2597C-Immunohistochemistry-Paraffin-NBP3-07075-img0001.jpg "Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (2597C) [NBP3-07075] - HIF-2 alpha /EPAS1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Human HIF-2 alpha /EPAS1 Monoclonal Antibody (Catalog # NBP3-07075) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.")
![Western Blot: Aconitase 2 Antibody [NBP1-52014] - (0.01ug/ml) staining of Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-52014-img0002.jpg "Western Blot: Aconitase 2 Antibody [NBP1-52014] - (0.01ug/ml) staining of Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-52014] - (3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-52014-img0003.jpg)
![Immunoprecipitation: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] - Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916). Western blot was performed from the immunoprecipitate using Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-p-Ser293-Antibody-9S3I4-Immunoprecipitation-NBP3-16916-img0002.jpg "Immunoprecipitation: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] - Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916). Western blot was performed from the immunoprecipitate using Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916) at a dilition of 1:1000.")
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] -analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb at1:23000 dilution.NIH/3T3 cells were treated by CIP (20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16916_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser-293-mab-6e3j0-western-blot-75202483541..jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] -analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb at 1:23000 dilution.293T cells were treated by lambda -PP mixed solution (1ul) at 30 c for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25uμg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16916_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser-293-mab-6e3j0-western-blot-75202483713..jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (6E3J0) [NBP3-16916] - Western blot analysis of lysates from HeLa cells, using Pyruvate Dehydrogenase E1-alpha subunit Rabbit mAb at 1:23000 dilution.293T cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16916_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser-293-mab-6e3j0-52202511383736.jpg)
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (9A7X4) [NBP3-16720] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase Kinase 1/PDK1/PDHK1 Rabbit mAb (NBP3-16720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-9A7X4-Western-Blot-NBP3-16720-img0001.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (9A7X4) [NBP3-16720] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase Kinase 1/PDK1/PDHK1 Rabbit mAb (NBP3-16720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8) [NBP3-16720] - Western blot analysis of various lysates, using Pyruvate Dehydrogenase Kinase 1/PDK1 Rabbit mAb at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16720_rabbit-pyruvate-dehydrogenase-kinase-1-pdk1-mab-5t6s8-52202511411497.jpg)
![Immunohistochemistry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8) [NBP3-16720] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using Pyruvate Dehydrogenase Kinase 1/PDK1 Rabbit mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16720_rabbit-pyruvate-dehydrogenase-kinase-1-pdk1-mab-5t6s8-5220251215491.jpg)
![Western Blot: PDK4 Antibody [NBP2-95194] - Western blot analysis of extracts of various cell lines, using PDK4 antibody (NBP2-95194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PDK4 Antibody-Western Blot-NBP2-95194-img0003.jpg "Western Blot: PDK4 Antibody [NBP2-95194] - Western blot analysis of extracts of various cell lines, using PDK4 antibody (NBP2-95194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-95194] - Immunohistochemistry of paraffin-embedded human placenta using PDK4 Rabbit pAb (NBP2-95194) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-95194-img0002.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-95194] - Immunohistochemistry of paraffin-embedded rat heart using PDK4 Rabbit pAb (NBP2-95194) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-95194-img0001.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - Lane 1.: Jurkat cell lysate Lane 2.: 293T cell lysate Lane 3.: A431 cell lysate. antibody (1:1000).](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-1A4-Western-Blot-NBP1-74023-img0008.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - Lane 1.: Jurkat cell lysate Lane 2.: 293T cell lysate Lane 3.: A431 cell lysate. antibody (1:1000).")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-1A4-Immunocytochemistry-Immunofluorescence-NBP1-74023-img0005.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody (8Z4N6) [NBP3-15470] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase E1-alpha subunit Rabbit mAb (NBP3-15470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-8Z4N6-Western-Blot-NBP3-15470-img0002.jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody (8Z4N6) [NBP3-15470] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase E1-alpha subunit Rabbit mAb (NBP3-15470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody (8Z4N6) [NBP3-15470] - Immunofluorescence analysis of NIH-3T3 cells using Pyruvate Dehydrogenase E1-alpha subunit Rabbit mAb (NBP3-15470) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-8Z4N6-Immunocytochemistry-Immunofluorescence-NBP3-15470-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody (8Z4N6) [Pyruvate Dehydrogenase E1-alpha subunit] - Confocal imaging of C2C12 cells using Pyruvate Dehydrogenase E1-alpha subunit Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15470_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-mab-8z4n6-522025171892.jpg)
![Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against HEK293 (1) and PPARGC1B (AA: 195-414)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-6C3F6-Western-Blot-NBP2-37249-img0003.jpg "Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against HEK293 (1) and PPARGC1B (AA: 195-414)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Flow cytometric analysis of Hela cells using PPARGC1B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-6C3F6-Flow-Cytometry-NBP2-37249-img0004.jpg)
![Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against human PPARGC1B (AA: 195-414) recombinant protein. (Expected MW is 50.3 kDa)](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-6C3F6-Western-Blot-NBP2-37249-img0002.jpg)
![ELISA: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-6C3F6-ELISA-NBP2-37249-img0001.jpg)
![Western Blot: CYB5R3 Antibody [NBP3-06497] - Lane A: A431 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: U87MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa](http://images.novusbio.com/fullsize/CYB5R3-Antibody-Western-Blot-NBP3-06497-img0001.jpg "Western Blot: CYB5R3 Antibody [NBP3-06497] - Lane A: A431 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: U87MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa")
![Immunohistochemistry-Paraffin: CYB5R3 Antibody [NBP3-06497] - Staining of human CYB5R3 in human testis with rabbit polyclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/CYB5R3-Antibody-Immunohistochemistry-Paraffin-NBP3-06497-img0002.jpg)
![Immunoprecipitation: CYB5R3 Antibody [NBP3-06497] - Lane A:0.5 mg A431 HepG2 HeLa U87MG Whole Cell Lysate4 uL anti-CYB5R3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CYB5R3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :34 kDa](http://images.novusbio.com/fullsize/CYB5R3-Antibody-Immunoprecipitation-NBP3-06497-img0003.jpg)
![Western Blot: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Western blot analysis of SREBP1 on different lysates with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 122 kDa Observed band size: 80-100 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32977) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32977_rabbit-srebp1-mab-psh04-61-288202412493476.jpg "Western Blot: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Western blot analysis of SREBP1 on different lysates with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 122 kDa Observed band size: 80-100 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32977) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32977) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32977_rabbit-srebp1-mab-psh04-61-2982024149263.jpg)
![Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Immunocytochemistry analysis of HeLa cells labeling SREBP1 with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32977_rabbit-srebp1-mab-psh04-61-29202410484870.jpg)