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![Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody [NBP3-35805] - Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35805_rabbit-tbc1d4-pab-912202414213796.jpg "Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody [NBP3-35805] - Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: TBC1D4 Antibody [NBP3-35805] - Western blot analysis of lysates from Rat skeletal muscle, using TBC1D4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35805_rabbit-tbc1d4-pab-912202415133469.jpg)
![Western Blot: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody (PSH01-38) [NBP3-32818] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 55/85 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32818) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32818_rabbit-pi-3-kinase-p85-alpha-p-tyr199-p-tyr467-mab-288202412442996.jpg "Western Blot: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody (PSH01-38) [NBP3-32818] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 55/85 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32818) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody (PSH01-38) [NBP3-32818] - Immunocytochemistry analysis of NIH/3T3 cells treated with or without 1mM Sodium orthovanadate for 30 minutes labeling PI 3-Kinase p85 alpha with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32818_rabbit-pi-3-kinase-p85-alpha-p-tyr199-p-tyr467-mab-29202410334087.jpg)
![Immunohistochemistry: ACSL5 Antibody (A9D4-R) [NBP3-31995] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (NBP3-31995) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31995) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31995_mouse-acsl5-mab-a9d4-r-29820241492626.jpg "Immunohistochemistry: ACSL5 Antibody (A9D4-R) [NBP3-31995] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (NBP3-31995) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31995) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: ACSL5 Antibody (A9D4-R) [NBP3-31995] - Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (NBP3-31995) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31995) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31995_mouse-acsl5-mab-a9d4-r-3920248453515.jpg)
![Western Blot: NT5C1A Antibody [NBP3-06431] - Anti-NT5C1A rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:44 kDa.](http://images.novusbio.com/fullsize/NT5C1A-Antibody-Western-Blot-NBP3-06431-img0003.jpg "Western Blot: NT5C1A Antibody [NBP3-06431] - Anti-NT5C1A rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:44 kDa.")
![Immunohistochemistry-Paraffin: NT5C1A Antibody [NBP3-06431] - Immunochemical staining of human NT5C1A in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NT5C1A-Antibody-Immunohistochemistry-Paraffin-NBP3-06431-img0002.jpg)
![Western Blot: Kif4A Antibody (JE38-91) [NBP3-32502] - Western blot analysis of Kif4A on different lysates with Rabbit anti-Kif4A antibody (NBP3-32502) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: SW480 cell lysate (20 ug/Lane) Lane 5: Mouse thymus tissue lysate (40 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Predicted band size: 140 kDa Observed band size: 160 kDa Exposure time: 6 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32502) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32502_rabbit-kif4a-mab-je38-91-27820241631627.jpg "Western Blot: Kif4A Antibody (JE38-91) [NBP3-32502] - Western blot analysis of Kif4A on different lysates with Rabbit anti-Kif4A antibody (NBP3-32502) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: SW480 cell lysate (20 ug/Lane) Lane 5: Mouse thymus tissue lysate (40 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Predicted band size: 140 kDa Observed band size: 160 kDa Exposure time: 6 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32502) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Kif4A Antibody (JE38-91) [NBP3-32502] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Kif4A antibody (NBP3-32502) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32502) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32502_rabbit-kif4a-mab-je38-91-29820241492699.jpg)
![Western Blot: Insulin Receptor beta Antibody [NB120-10991] - Representative lot data. Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Insulin Receptor, b subunit (1:1,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor, b subunit (~97kDa)](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-Western-Blot-NB120-10991-img0005.jpg "Western Blot: Insulin Receptor beta Antibody [NB120-10991] - Representative lot data. Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Insulin Receptor, b subunit (1:1,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor, b subunit (~97kDa)")
![Immunoprecipitation: Insulin Receptor beta Antibody [NB120-10991] - Immunoprecipitation 10 ug of this antibody immunoprecipitated a-Insulin Receptor, Beta subunit from 500 micrograms of Hek293 cell lysate using protein A agarose beads. Arrow indicates a-Insulin Receptor, Beta subunit (97 kDa).](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-Immunoprecipitation-NB120-10991-img0004.jpg)
![Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456] - Detection of GAPDHS in acrosomes of human sperms with anti-GAPDHS (Hs-8) FITC.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Hs-8-Flow-Cytometry-NB500-456-img0001.jpg "Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456] - Detection of GAPDHS in acrosomes of human sperms with anti-GAPDHS (Hs-8) FITC.")
![Immunohistochemistry-Paraffin: Apolipoprotein C1 Antibody [NBP1-06016] - IHC staining of Apolipoprotein C1 in paraffin embedded Human Liver using this antibody at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Apolipoprotein-C1-Antibody-Immunohistochemistry-Paraffin-NBP1-06016-img0002.jpg "Immunohistochemistry-Paraffin: Apolipoprotein C1 Antibody [NBP1-06016] - IHC staining of Apolipoprotein C1 in paraffin embedded Human Liver using this antibody at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: CHFR Antibody [NB100-1166] - IHC analysis of CHFR in paraffin-embedded Human Brain (Cerebellum) using this antibody at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CHFR-Antibody-Immunohistochemistry-Paraffin-NB100-1166-img0002.jpg "Immunohistochemistry-Paraffin: CHFR Antibody [NB100-1166] - IHC analysis of CHFR in paraffin-embedded Human Brain (Cerebellum) using this antibody at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: ABCB9 Antibody [NB300-865] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining](http://images.novusbio.com/fullsize/ABCB9-Antibody-Immunohistochemistry-Paraffin-NB300-865-img0002.jpg "Immunohistochemistry-Paraffin: ABCB9 Antibody [NB300-865] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining")
![Western Blot: Apolipoprotein H/ApoH Antibody [NBP1-97764] - NBP1-97764 (0.2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Apolipoprotein-H-ApoH-Antibody-Western-Blot-NBP1-97764-img0003.jpg "Western Blot: Apolipoprotein H/ApoH Antibody [NBP1-97764] - NBP1-97764 (0.2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")