| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27342SS | |||
| Azide and BSA Free | NBP2-80791 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB15651-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-88930 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87293-25ul | |||
![Immunohistochemistry: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32496_rabbit-ketohexokinase-mab-psh03-18-29820241420952.jpg "Immunohistochemistry: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Immunocytochemistry analysis of HepG2 cells labeling Ketohexokinase with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32496_rabbit-ketohexokinase-mab-psh03-18-2920241192372.jpg)
![Flow Cytometry: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Flow cytometric analysis of HepG2 cells labeling Ketohexokinase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32496, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32496_rabbit-ketohexokinase-mab-psh03-18-39202488461.jpg)
![Western Blot: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Western blot analysis of Ketohexokinase on different lysates with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/2,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Human kidney tissue lysate Lane 3: Mouse liver tissue lysate Lane 4: Mouse kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32496_rabbit-ketohexokinase-mab-psh03-18-392024845350.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N1G6) [NBP2-27342] - Analysis of IL-1beta in A) full-length recombinant protein and B) human spleen lysate using IL-1beta antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N1G6-Western-Blot-NBP2-27342-img0002.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (43N1G6) [NBP2-27342] - Analysis of IL-1beta in A) full-length recombinant protein and B) human spleen lysate using IL-1beta antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Frozen: FABP1/L-FABP Antibody (966927) [MAB15651] - Antibody was detected in immersion fixed Frozen-embedded sections of rat intestine tissue.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-966927-Immunohistochemistry-Frozen-MAB15651-img0001.jpg "Immunohistochemistry-Frozen: FABP1/L-FABP Antibody (966927) [MAB15651] - Antibody was detected in immersion fixed Frozen-embedded sections of rat intestine tissue.")
![Western Blot: GAPDH Antibody (RM114) [NBP2-77402] - Western blot of A431 cells, using NBP2-77402, showed a band of GAPDH at the predicted MW (~36 kDa).](http://images.novusbio.com/fullsize/GAPDH-Antibody-RM114-Western-Blot-NBP2-77402-img0002.jpg "Western Blot: GAPDH Antibody (RM114) [NBP2-77402] - Western blot of A431 cells, using NBP2-77402, showed a band of GAPDH at the predicted MW (~36 kDa).")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (RM114) [NBP2-77402] - Immunocytochemical staining of HeLa cells, using NBP2-77402 (red). Nuclei have been labeled with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-77402_rabbit-monoclonal-gapdh-antibody-rm114-immunocytochemistry-immunofluorescence-199202502434.png)
![Western Blot: Fumarylacetoacetate hydrolase Antibody (HL1970) - Azide and BSA Free [NBP3-25460] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1970] (NBP3-25460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25460_rabbit-fumarylacetoacetate-hydrolase-mab-hl1970-azide-bsa-free-21202494143.jpg "Western Blot: Fumarylacetoacetate hydrolase Antibody (HL1970) - Azide and BSA Free [NBP3-25460] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1970] (NBP3-25460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Fumarylacetoacetate hydrolase Antibody (HL1970) - Azide and BSA Free [NBP3-25460] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1970] (NBP3-25460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25460_rabbit-fumarylacetoacetate-hydrolase-mab-hl1970-azide-bsa-free-2120249403860.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Fumarylacetoacetate hydrolase Antibody (HL1970) - Azide and BSA Free [NBP3-25460] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1970] (NBP3-25460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25460_rabbit-fumarylacetoacetate-hydrolase-mab-hl1970-azide-bsa-free-2120249351393.jpg)
![Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody (HL1970) - Azide and BSA Free [NBP3-25460] - FAH antibody [HL1970] detects FAH protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. FAH stained by FAH antibody [HL1970] (NBP3-25460) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25460_rabbit-fumarylacetoacetate-hydrolase-mab-hl1970-azide-bsa-free-2120249342746.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Fumarylacetoacetate hydrolase Antibody (HL1970) - Azide and BSA Free [NBP3-25460] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1970] (NBP3-25460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25460_rabbit-fumarylacetoacetate-hydrolase-mab-hl1970-azide-bsa-free-2120249413351.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-2120249432858.jpg "Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-21202494149.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-212024941459.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - Wild-type (WT) and PFKM knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-2120249422638.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Muscle Phosphofructokinase/PFKM/PFK-1 antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-western-blot-1411202518312123.jpg)
![Western Blot: FACL4 Antibody (GT566) [NBP3-13526] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT566] (NBP3-13526) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-GT566-Western-Blot-NBP3-13526-img0005.jpg "Western Blot: FACL4 Antibody (GT566) [NBP3-13526] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT566] (NBP3-13526) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: FACL4 Antibody (GT566) [NBP3-13526] - FACL4 antibody [GT566] detects FACL4 protein at cytoplasm in rat pancreas by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. FACL4 antibody [GT566] (NBP3-13526) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/FACL4-Antibody-GT566-Immunohistochemistry-Paraffin-NBP3-13526-img0003.jpg)
![Western Blot: FACL4 Antibody (GT566) [NBP3-13526] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT566] (NBP3-13526) diluted at 1:500. The signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/FACL4-Antibody-GT566-Western-Blot-NBP3-13526-img0001.jpg)
![Western Blot: FACL4 Antibody (GT566) [NBP3-13526] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT566] (NBP3-13526) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/FACL4-Antibody-GT566-Western-Blot-NBP3-13526-img0004.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody (GT566) [NBP3-13526] - FACL4 antibody [GT566] detects FACL4 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. FACL4 antibody [GT566] (NBP3-13526) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/FACL4-Antibody-GT566-Immunohistochemistry-Paraffin-NBP3-13526-img0002.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP1-87293-img0012.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87293-img0013.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87293-img0010.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in human skeletal muscle tissue.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP1-87293-img0011.jpg)
![Western Blot: Lipocalin-2/NGAL Antibody [NB100-1503] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-Western-Blot-NB100-1503-img0008.jpg "Western Blot: Lipocalin-2/NGAL Antibody [NB100-1503] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503] - Analysis of LCN2 in human xenograft tumor. Image courtesy of product review submitted by Yi-Ling Huang.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-Immunohistochemistry-Paraffin-NB100-1503-img0006.jpg)
![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-Immunohistochemistry-Paraffin-NB100-1503-img0005.jpg)
![Western Blot: MRP2 Antibody [NBP1-69023] - Sample Tissue: Mouse Thymus Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/MRP2-Antibody-Western-Blot-NBP1-69023-img0004.jpg "Western Blot: MRP2 Antibody [NBP1-69023] - Sample Tissue: Mouse Thymus Antibody Dilution: 1.0ug/ml")
![Western Blot: MRP2 Antibody [NBP1-69023] - Mouse Spleen lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/MRP2-Antibody-Western-Blot-NBP1-69023-img0003.jpg)
![Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220] - Analysis of extracts of various cell lines, using COX-2 antibody (NBP3-16220) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/COX-2-Antibody-2V7G8-Western-Blot-NBP3-16220-img0002.jpg "Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220] - Analysis of extracts of various cell lines, using COX-2 antibody (NBP3-16220) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Human colon carcinoma tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16220_rabbit-cox-2-mab-2v7g8-immunohistochemistry-paraffin-155202485950..jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Human colon tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16220_rabbit-cox-2-mab-2v7g8-immunohistochemistry-paraffin-15520249058..jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Aanalysis of COX2/PTGS2 in paraffin-embedded mouse intestin tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16220_rabbit-cox-2-mab-2v7g8-immunohistochemistry-paraffin-15520249247..jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] - Analysis of COX2/PTGS2 in paraffin-embedded rat colon tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16220_rabbit-cox-2-mab-2v7g8-immunohistochemistry-paraffin-15520249513.jpg)