| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43817 | |||
| BSA Free | NBP2-46646-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26636-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77556SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77559SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48998-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26637-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26218-50ul | |||
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining in human testis and pancreas tissues.. Corresponding CYP17A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NBP2-13892-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining in human testis and pancreas tissues.. Corresponding CYP17A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cytochrome P450 17A1 Antibody [NBP2-13892] - Analysis in human adrenal gland tissue.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Western-Blot-NBP2-13892-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining of human pancreas shows no positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NBP2-13892-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NBP2-13892-img0011.jpg)
![Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of rat midbrain shows strong positivity in serotonin neurons in the dorsal raphe nucleus.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-2990-Immunohistochemistry-Paraffin-NBP2-46646-img0016.jpg "Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of rat midbrain shows strong positivity in serotonin neurons in the dorsal raphe nucleus.")
![Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of mouse brain shows moderate positivity in serotonergic fibers in the basal forebrain.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-2990-Immunohistochemistry-Paraffin-NBP2-46646-img0011.jpg)
![Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of mouse brain shows moderate positivity in serotonergic fibers in the cerebral cortex.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-2990-Immunohistochemistry-Paraffin-NBP2-46646-img0012.jpg)
![Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of human dorsal raphe nucleus shows strong cytoplasmic positivity in serotonin neurons.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-2990-Immunohistochemistry-Paraffin-NBP2-46646-img0013.jpg)
![Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of human cerebral cortex shows moderate positivity in serotonergic neural fibers.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-2990-Immunohistochemistry-Paraffin-NBP2-46646-img0014.jpg)
![Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of human kidney shows no positivity in cells in tubules or glomeruli as expected.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-2990-Immunohistochemistry-Paraffin-NBP2-46646-img0015.jpg)
![Western Blot: Aromatase Antibody (5C5N7) [NBP3-15438] - Western blot analysis of extracts of U-87MG cells, using Aromatase antibody (NBP3-15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Western-Blot-NBP3-15438-img0003.jpg "Western Blot: Aromatase Antibody (5C5N7) [NBP3-15438] - Western blot analysis of extracts of U-87MG cells, using Aromatase antibody (NBP3-15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry: Aromatase Antibody (5C5N7) [NBP3-15438] - Immunofluorescence analysis of human placenta using Aromatase Rabbit mAb (NBP3-15438) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Immunohistochemistry-NBP3-15438-img0002.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody (5C5N7) [NBP3-15438] - Human colon using Aromatase Rabbit mAb (NBP3-15438) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Immunohistochemistry-Paraffin-NBP3-15438-img0001.jpg)
![Immunohistochemistry: Aromatase Antibody (5C5N7) [Aromatase] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Aromatase Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15438_rabbit-aromatase-mab-5c5n7-52202517273588.jpg)
![Immunohistochemistry: Aromatase Antibody (5C5N7) [Aromatase] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Aromatase Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15438_rabbit-aromatase-mab-5c5n7-5220251723570.jpg)
![Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - NBP3-26636 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101148.jpg "Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - NBP3-26636 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody")
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of Hela cell with NBP3-26636 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101147.jpg)
![Western Blot: Aromatase Antibody (1H1) [NBP3-26636] - Positive WB detected in: 293 whole cell lysate (20µg), K562 whole cell lysate (20µg), Hela whole cell lysate (20µg), MCF-7 whole cell lysate (20µg), PC-3 whole cell lysate (20µg)All lanes: CYP19A1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 kDaObserved band size: 58 kDaExposure time：120s](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101143.jpg)
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-26120251011416.jpg)
![Western Blot: Tryptophan hydroxylase 2 Antibody [NBP1-77556] - Analysis of TPHZ in human stomach lysate.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Western-Blot-NBP1-77556-img0001.jpg "Western Blot: Tryptophan hydroxylase 2 Antibody [NBP1-77556] - Analysis of TPHZ in human stomach lysate.")
![Western Blot: Tryptophan hydroxylase 2 Antibody [NBP1-77559] - Analysis of TPH2 in human stomach lysate.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Western-Blot-NBP1-77559-img0001.jpg "Western Blot: Tryptophan hydroxylase 2 Antibody [NBP1-77559] - Analysis of TPH2 in human stomach lysate.")
![Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining in human placenta and prostate tissues using anti-CYP19A1 antibody. Corresponding CYP19A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NBP2-48998-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining in human placenta and prostate tissues using anti-CYP19A1 antibody. Corresponding CYP19A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NBP2-48998] - Staining of human cell line ASC TERT1 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48998-img0008.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NBP2-48998-img0004.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining of human prostate shows low expression as expected.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NBP2-48998-img0005.jpg)
![Immunohistochemistry-Frozen: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Immunofluorescence analysis of frozen mouse brain (raphe nucleus) tissue with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33037, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33037_rabbit-tryptophan-hydroxylase-2-mab-psh04-89-2682024159393.jpg "Immunohistochemistry-Frozen: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Immunofluorescence analysis of frozen mouse brain (raphe nucleus) tissue with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33037, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Western blot analysis of Tryptophan hydroxylase 2 on different lysates with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/1,000 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 18 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33037) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33037_rabbit-tryptophan-hydroxylase-2-mab-psh04-89-28820241249341.jpg)
![Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33037) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33037_rabbit-tryptophan-hydroxylase-2-mab-psh04-89-29820241492660.jpg)
![Immunohistochemistry: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Image of Cytochrome P450 17A1 Antibody (5A9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-2722024831378.jpg "Immunohistochemistry: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Image of Cytochrome P450 17A1 Antibody (5A9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Positive Western Blot detected in: HepG2 whole cell lysate, Rat Heart whole cell lysate, Rat Brain whole cell lysate. All lanes: Cytochrome P450 17A1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58 kDa Observed band size: 58 kDa](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-28220241140920.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Staining of Hela Cells with Cytochrome P450 17A1 Antibody (5A9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-282202412475149.jpg)
![Immunohistochemistry: HSD3B1 Antibody (10G2) [NBP3-26218] - Image of HSD3B1 Antibody (10G2) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.50% DAB.](http://images.novusbio.com/fullsize/nbp3-26218_rabbit-hsd3b1-mab-10g2-2622024152246.jpg "Immunohistochemistry: HSD3B1 Antibody (10G2) [NBP3-26218] - Image of HSD3B1 Antibody (10G2) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.50% DAB.")
![Immunohistochemistry: HSD3B1 Antibody (10G2) [NBP3-26218] - Image of HSD3B1 Antibody (10G2) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.50% DAB.](http://images.novusbio.com/fullsize/nbp3-26218_rabbit-hsd3b1-mab-10g2-2722024856317.jpg)
![Immunocytochemistry/Immunofluorescence: HSD3B1 Antibody (10G2) [NBP3-26218] - Staining of Hela with HSD3B1 Antibody (10G2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 514-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26218_rabbit-hsd3b1-mab-10g2-282202412475114.jpg)
![Flow Cytometry: HSD3B1 Antibody (10G2) [NBP3-26218] - Overlay Peak curve showing 293 cells stained with HSD3B1 Antibody (10G2) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26218_rabbit-hsd3b1-mab-10g2-282202414183666.jpg)
![Western Blot: CYP11B2 Antibody [NBP1-56518] - Transfected HEK293-CYP11B2, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Western-Blot-NBP1-56518-img0003.jpg "Western Blot: CYP11B2 Antibody [NBP1-56518] - Transfected HEK293-CYP11B2, Antibody Titration: 0.2-1 ug/ml")
![Western Blot: CYP11B2 Antibody [NBP1-56518] - Sample type: 1. HEK293TN-GFP-hcyp11B1 (75ug) 2. HEK293TN-GFP-hcyp11B2 (75ug) Primary Dilution: 1:100 Secondary Antibody: mouse anti-Rabbit HRP Secondary Dilution: 1:10,000 Film Exposed for: 15 minutes](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Western-Blot-NBP1-56518-img0002.jpg)