| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75923SS | |||
| Azide and BSA Free | NBP2-80731 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38499-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87169-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70166 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73228 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43875 | |||
![Flow Cytometry: Ferroportin/SLC40A1 Antibody (1308C) [NBP2-75923] - HEK293 human embryonic kidney cell line transfected with human Ferroportin/SLC40A1 and eGFP was stained with either (A) Rabbit Anti-Human Ferroportin/SLC40A1 Antibody (1308C) (NBP2-75923) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).](http://images.novusbio.com/fullsize/Ferroportin-SLC40A1-Antibody-1308C-Flow-Cytometry-NBP2-75923-img0001.jpg "Flow Cytometry: Ferroportin/SLC40A1 Antibody (1308C) [NBP2-75923] - HEK293 human embryonic kidney cell line transfected with human Ferroportin/SLC40A1 and eGFP was stained with either (A) Rabbit Anti-Human Ferroportin/SLC40A1 Antibody (1308C) (NBP2-75923) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).")
![Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody (1308C) [NBP2-75923] - Ferroportin/SLC40A1 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Ferroportin/SLC40A1 Antibody (1308C) (Catalog # NBP2-75923) at 1 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Kupffer cells.](http://images.novusbio.com/fullsize/Ferroportin-SLC40A1-Antibody-1308C-Immunohistochemistry-Paraffin-NBP2-75923-img0002.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Analysis in human bone marrow and kidney tissues. Corresponding PADI4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Analysis in human bone marrow and kidney tissues. Corresponding PADI4 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human bone marrow, liver, spleen and testis using Anti-PADI4 antibody NBP1-87353 (A) shows similar protein distribution across tissues to independent antibody NBP2-38499 (B).](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0019.jpg)
![Western Blot: PADI4 Antibody [NBP1-87353] - Analysis in control (vector only transfected HEK293T lysate) and pADI4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/PADI4-Antibody-Western-Blot-NBP1-87353-img0010.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human testis shows no nuclear positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0023.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0018.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human kidney shows no nuclear positivity as expected.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0020.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0021.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human spleen shows strong nuclear positivity in cells in red pulp.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0022.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining in human bone marrow and kidney tissues using anti-PADI4 antibody. Corresponding PADI4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining in human bone marrow and kidney tissues using anti-PADI4 antibody. Corresponding PADI4 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human bone marrow, liver, spleen and testis using Anti-PADI4 antibody NBP2-38499 (A) shows similar protein distribution across tissues to independent antibody NBP1-87353 (B).](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0012.jpg)
![Western Blot: PADI4 Antibody [NBP2-38499] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/PADI4-Antibody-Western-Blot-NBP2-38499-img0006.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0003.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0004.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human liver.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0008.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human testis.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0009.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human spleen.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0010.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human bone marrow using Anti-PADI4 antibody NBP2-38499.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0011.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP1-87169] - Analysis using Anti-AMACR antibody NBP1-87169 (A) shows similar pattern to independent antibody NBP1-87168 (B).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-Western-Blot-NBP1-87169-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP1-87169] - Analysis using Anti-AMACR antibody NBP1-87169 (A) shows similar pattern to independent antibody NBP1-87168 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP1-87169] - Staining of human pancreas using Anti-AMACR antibody NBP1-87169.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-Immunohistochemistry-Paraffin-NBP1-87169-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP1-87169] - Analysis in human cell lines Caco-2 and U2OS. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-Western-Blot-NBP1-87169-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP1-87169] - Staining in human kidney and pancreas tissues using anti-AMACR antibody. Corresponding AMACR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-Immunohistochemistry-Paraffin-NBP1-87169-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP1-87169] - Staining of human kidney, liver, lymph node and pancreas using Anti-AMACR antibody NBP1-87169 (A) shows similar protein distribution across tissues to independent antibody NBP1-87168 (B).](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-Immunohistochemistry-Paraffin-NBP1-87169-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP1-87169] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-Immunohistochemistry-Paraffin-NBP1-87169-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP1-87169] - Staining of human liver.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-Immunohistochemistry-Paraffin-NBP1-87169-img0012.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP1-87169] - Staining of human lymph node.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-Immunohistochemistry-Paraffin-NBP1-87169-img0013.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP1-87169] - Staining of human kidney using Anti-AMACR antibody NBP1-87169.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-Immunohistochemistry-Paraffin-NBP1-87169-img0014.jpg)
![Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Western-Blot-NBP2-46513-img0003.jpg "Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)")
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Adenocarcinoma of Human ovary tissue using antiALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0014.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human colon tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human Kidney tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0007.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Carcinoma of Human kidney tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min (1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0010.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human liver tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0015.jpg)
![Western Blot: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Western-Blot-NBP2-46310-img0008.jpg "Western Blot: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.")
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0007.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0001.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0002.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0003.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) [NBP2-46310] - Human Ovary tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120 degrees C for 3min.](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0004.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0005.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0006.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) [NBP2-88930] - Analysis in human cell line CACO-2.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9360-Western-Blot-NBP2-88930-img0007.jpg "Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) [NBP2-88930] - Analysis in human cell line CACO-2.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) [NBP2-88930] - Analysis in human kidney and skeletal muscle tissues using NBP2-88930 antibody. Corresponding AMACR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9360-Immunohistochemistry-Paraffin-NBP2-88930-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) [NBP2-88930] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9360-Immunohistochemistry-Paraffin-NBP2-88930-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) [NBP2-88930] - Staining of human liver cancer shows moderate granular cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9360-Immunohistochemistry-Paraffin-NBP2-88930-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) [NBP2-88930] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9360-Immunohistochemistry-Paraffin-NBP2-88930-img0003.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) [NBP2-88930] - Staining of human pancreas shows weak granular cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9360-Immunohistochemistry-Paraffin-NBP2-88930-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) [NBP2-88930] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/alpha-Methylacyl-CoA-Racemase-AMACR-Antibody-CL9360-Immunohistochemistry-Paraffin-NBP2-88930-img0005.jpg)
![Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Western-Blot-NBP3-16840-img0005.jpg "Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of mouse eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0004.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Rat retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0001.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Mouse retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0002.jpg)
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of rat eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of paraffin-embedded rat eye using RPE65 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16840_rabbit-rpe65-mab-5x1f6-52202511361295.jpg)
![Western Blot: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Western blot analysis of Tyrosinase on B16F1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-JA52-11-Western-Blot-NBP2-67232-img0006.jpg "Western Blot: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Western blot analysis of Tyrosinase on B16F1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Staining Tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-JA52-11-Immunocytochemistry-Immunofluorescence-NBP2-67232-img0005.jpg)
![Immunohistochemistry-Paraffin: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Analysis of paraffin-embedded human stomach cancer tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-JA52-11-Immunohistochemistry-Paraffin-NBP2-67232-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Staining Tyrosinase in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-JA52-11-Immunocytochemistry-Immunofluorescence-NBP2-67232-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Staining Tyrosinase in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-JA52-11-Immunocytochemistry-Immunofluorescence-NBP2-67232-img0002.jpg)
![Immunohistochemistry-Paraffin: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Analysis of paraffin-embedded human liver tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-JA52-11-Immunohistochemistry-Paraffin-NBP2-67232-img0003.jpg)
![Western Blot: Tyrosinase Antibody (C2-B5) [NBP2-67233] - Analysis of tyrosinase on melanoma cells lysate using anti- tyrosinase antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-C2-B5-Western-Blot-NBP2-67233-img0003.jpg "Western Blot: Tyrosinase Antibody (C2-B5) [NBP2-67233] - Analysis of tyrosinase on melanoma cells lysate using anti- tyrosinase antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Tyrosinase Antibody (C2-B5) [NBP2-67233] - Staining tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-C2-B5-Immunocytochemistry-Immunofluorescence-NBP2-67233-img0004.jpg)
![Immunohistochemistry-Paraffin: Tyrosinase Antibody (C2-B5) [NBP2-67233] - Analysis of paraffin-embedded human tyrosinase tissue using anti- tyrosinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-C2-B5-Immunohistochemistry-Paraffin-NBP2-67233-img0002.jpg)
![Flow Cytometry: Tyrosinase Antibody (C2-B5) [NBP2-67233] - Analysis of B16F1 cells with tyrosinase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-C2-B5-Flow-Cytometry-NBP2-67233-img0001.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - detection against Immunogen (38.1 KDa) .](http://images.novusbio.com/fullsize/PADI4-Antibody-4D8-Western-Blot-H00023569-M01-img0001.jpg "Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - detection against Immunogen (38.1 KDa) .")
![Immunocytochemistry/Immunofluorescence: PADI4 Antibody (4D8) [H00023569-M01] - Analysis of monoclonal antibody to PADI4 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PADI4-Antibody-4D8-Immunocytochemistry-Immunofluorescence-H00023569-M01-img0004.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - Detection of enzymatically active peptidyl arginine deiminase (PAD) in the synovial fluid (SF) of patients with rheumatoid arthritis (RA). A, Immunoblotting of albumin‐depleted SF samples from 8 RA patients shows presence of neutrophil elastase (NE), PAD4, & PAD2. B, PAD activity in the SF of patients with RA (n = 12) was significantly higher than that in the SF of patients with osteoarthritis (OA) (n = 9). C, PAD activity was observed in the OA SF & RA SF both at physiologic calcium concentrations & at higher calcium concentrations (5 mM). In B & C, symbols represent individual patients; horizontal bars show the median. D, PAD activity is significantly correlated with DNA levels in the SF of RA patients (n = 22). Data were analyzed using Spearman's rho test for correlation. ∗ = P < 0.05; ∗∗ = P < 0.01, by Mann‐Whitney U test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26245941), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023569-m01_mouse-monoclonal-padi4-antibody-4d8-310202415482220.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - Release of peptidyl arginine deiminases (PADs) into the supernatant during NETosis, & detection of enzymatically active PADs. A, DNA release in peripheral blood neutrophils from healthy donors that were left unstimulated or stimulated with 25 mM phorbol myristate acetate (PMA) was assessed for up to 240 minutes after stimulation. Bars show the mean ± SD of 8 samples per group. B, For isolation of neutrophil extracellular traps, supernatants (SN) of unstimulated (unst.) or stimulated (stim.) neutrophils were collected. Subsequently, the cells were washed 3 times with RPMI medium (W1–W3), & stimulated cells were incubated in the absence or presence of DNase I; unstimulated cells treated with DNase I served as a control. Bars show the mean ± SD DNA concentration (conc) in 7 samples per group. C, Proteins (PAD2, PAD4, & neutrophil elastase [NE]) were precipitated from the same supernatants of unstimulated or stimulated cells as described in B & analyzed by Western blotting. Citrullinated proteins (citr.prot.) were detected using chemical modification & anti–modified citrulline antibody. PAD2 & PAD4 antibodies were tested for cross‐reactivity using human (hu) recombinant PAD4 (rPAD4) (250 ng) & human skeletal muscle tissue lysate (15 μg). One representative blot of 4 independent experiments is shown for each group. D, PAD activity in supernatants of unstimulated & stimulated cells was compared. Symbols represent individual donors (n = 10); bars show the median. ∗ = P < 0.05; ∗∗ = P < 0.01, by Wilcoxon's matched pairs signed rank test. AFU = arbitrary fluorescence units; NS = not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26245941), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023569-m01_mouse-monoclonal-padi4-antibody-4d8-310202415485135.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249403823.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249374927.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249351323.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249393597.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-212024940823.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-212024940846.jpg)