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![Western Blot: SAT2 Antibody [NBP1-80722] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/SAT2-Antibody-Western-Blot-NBP1-80722-img0005.jpg "Western Blot: SAT2 Antibody [NBP1-80722] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: SAT2 Antibody [NBP1-80722] - Staining of human colon.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80722-img0011.jpg)
![Immunohistochemistry-Paraffin: SAT2 Antibody [NBP1-80722] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80722-img0008.jpg)
![Immunohistochemistry-Paraffin: SAT2 Antibody [NBP1-80722] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80722-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SAT2 Antibody [NBP1-80722] - Staining in human adrenal gland and pancreas tissues using anti-SAT2 antibody. Corresponding SAT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80722-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SAT2 Antibody [NBP1-80722] - Staining of human adrenal gland, colon, pancreas and small intestine using Anti-SAT2 antibody NBP1-80722 (A) shows similar protein distribution across tissues to independent antibody NBP2-49244 (B).](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80722-img0010.jpg)
![Immunohistochemistry-Paraffin: SAT2 Antibody [NBP1-80722] - Staining of human small intestine.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80722-img0012.jpg)
![Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-0P3H3-Western-Blot-NBP3-16426-img0005.jpg "Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-0P3H3-Western-Blot-NBP3-16426-img0006.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Aconitase 2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16426_rabbit-aconitase-2-mab-0p3h3-5220251242556.jpg)
![Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunofluorescence analysis of NIH-3T3 cells using Aconitase 2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16426_rabbit-aconitase-2-mab-0p3h3-5220251242512.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Aconitase 2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16426_rabbit-aconitase-2-mab-0p3h3-52202511383784.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Aconitase 2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16426_rabbit-aconitase-2-mab-0p3h3-52202511361218.jpg)
![Western Blot: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70074-img0001.jpg "Western Blot: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase")
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Aconitase 2.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70074-img0003.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Kidney tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70074-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of HepG2 cells using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70074-img0002.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70074-img0004.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Ovary tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70074-img0006.jpg)
![Western Blot: ECH1 Antibody [NBP2-16261] - Wild-type (WT) and ECH1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ECH1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ECH1-Antibody-Western-Blot-NBP2-16261-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ECH1 Antibody [NBP2-16261] - Wild-type (WT) and ECH1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ECH1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ECH1 Antibody [NBP2-16261] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ECH1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/ECH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16261-img0003.jpg)
![Immunohistochemistry-Paraffin: ECH1 Antibody [NBP2-16261] - ECH1 antibody detects ECH1 protein at mitochondrion on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. ECH1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECH1-Antibody-Immunohistochemistry-Paraffin-NBP2-16261-img0004.jpg)
![Western Blot: ECH1 Antibody [NBP2-16261] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ECH1-Antibody-Western-Blot-NBP2-16261-img0001.jpg)
![Western Blot: ECH1 Antibody [NBP2-16261] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ECH1-Antibody-Western-Blot-NBP2-16261-img0002.jpg)
![Western Blot: Ketohexokinase Antibody [NBP1-32778] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Western-Blot-NBP1-32778-img0009.jpg "Western Blot: Ketohexokinase Antibody [NBP1-32778] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: ketohexokinase Antibody [NBP1-32778] - Sample (20 ug of whole cell lysate) A: mouse Liver 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/ketohexokinase-Antibody-Western-Blot-NBP1-32778-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Ketohexokinase Antibody [NBP1-32778] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Western-Blot-NBP1-32778-img0007.jpg)
![Immunohistochemistry-Paraffin: ketohexokinase Antibody [NBP1-32778] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-32778-img0002.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-32778] - Paraffin-embedded mouse kidney. Ketohexokinase antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-32778-img0005.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-32778] - Ketohexokinase antibody [N1C3] detects Ketohexokinase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Ketohexokinase stained by Ketohexokinase antibody [N1C3] (NBP1-32778) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32778_rabbit-polyclonal-ketohexokinase-antibody-91020242021965.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-52015-img0005.jpg "Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-52015] - (3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-52015-img0002.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Aconitase 2 (aa541-555) in Heart lysate (35ug protein in RIPA buffer) using this antibody at 0.01ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-52015-img0003.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Human (A), Mouse (B) and Rat (C) Adipose lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-52015-img0004.jpg)
![Western Blot: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Western blot shows lysates of TIME human endothelial parental cell line and ABCD1 knockout (KO) TIME cell line. PVDF membrane was probed with 1:2000 of Mouse Anti-Human ABCD1 Monoclonal Antibody (Catalog # NBP2-46476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for ABCD1 at approximately 75 kDa (as indicated) in the parental TIME cell line, but is not detectable in the knockout TIME cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Knockdown-Validated-NBP2-46476-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Western blot shows lysates of TIME human endothelial parental cell line and ABCD1 knockout (KO) TIME cell line. PVDF membrane was probed with 1:2000 of Mouse Anti-Human ABCD1 Monoclonal Antibody (Catalog # NBP2-46476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for ABCD1 at approximately 75 kDa (as indicated) in the parental TIME cell line, but is not detectable in the knockout TIME cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Western-Blot-NBP2-46476-img0004.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Immunohistochemistry-NBP2-46476-img0003.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Immunohistochemistry-NBP2-46476-img0001.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Immunohistochemistry-NBP2-46476-img0002.jpg)
![Western Blot: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Western-Blot-NBP2-70150-img0001.jpg "Western Blot: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1.")
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70150-img0004.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70150-img0002.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70150-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Western blot shows lysates of TIME human endothelial parental cell line and ABCD1 knockout (KO) TIME cell line. PVDF membrane was probed with 1:2000 of Mouse Anti-Human ABCD1 Monoclonal Antibody (Catalog # NBP2-46476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for ABCD1 at approximately 75 kDa (as indicated) in the parental TIME cell line, but is not detectable in the knockout TIME cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Knockout-Validated-NBP2-70150-img0005.jpg)
![Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Analysis of KHK expression in transfected 293T cell line by KHK monoclonal antibody (M01), clone 1H6-2B6.Lane 1: KHK transfected lysate(32.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1H6-2B6-Western-Blot-H00003795-M01-img0019.jpg "Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Analysis of KHK expression in transfected 293T cell line by KHK monoclonal antibody (M01), clone 1H6-2B6.Lane 1: KHK transfected lysate(32.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Analysis of monoclonal antibody to KHK on formalin-fixed paraffin-embedded human spleen tissue. Antibody concentration 1 ~ 10 ug/ml.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1H6-2B6-Immunohistochemistry-Paraffin-H00003795-M01-img0015.jpg)
![Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - KHK expression in human spleen.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1H6-2B6-Western-Blot-H00003795-M01-img0012.jpg)
![Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Analysis of KHK over-expressed 293 cell line, cotransfected with KHK Validated Chimera RNAi ( Cat # H00003795-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KHK monoclonal antibody (M01), clone 1H6-2B6 (Cat # H00003795-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1H6-2B6-Western-Blot-H00003795-M01-img0016.jpg)
![Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - KHK monoclonal antibody (M01), clone 1H6-2B6. Analysis of KHK expression in human liver.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1H6-2B6-Western-Blot-H00003795-M01-img0018.jpg)
![Sandwich ELISA: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Detection limit for recombinant GST tagged KHK is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1H6-2B6-Sandwich-ELISA-H00003795-M01-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: GALE Antibody [NBP1-87066] - Staining of human cell line A-431 shows localization to nucleus, plasma membrane & cytosol.](http://images.novusbio.com/fullsize/GALE-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87066-img0008.jpg "Immunocytochemistry/Immunofluorescence: GALE Antibody [NBP1-87066] - Staining of human cell line A-431 shows localization to nucleus, plasma membrane & cytosol.")
![Immunohistochemistry-Paraffin: GALE Antibody [NBP1-87066] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/GALE-Antibody-Immunohistochemistry-Paraffin-NBP1-87066-img0001.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-87066_rabbit-polyclonal-gale-antibody-304202513101484.jpg)
![Immunohistochemistry-Paraffin: SAT2 Antibody [NBP2-49244] - Staining of human colon.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP2-49244-img0006.jpg "Immunohistochemistry-Paraffin: SAT2 Antibody [NBP2-49244] - Staining of human colon.")
![Immunohistochemistry: SAT2 Antibody [NBP2-49244] - Staining of human kidney shows cytoplasmic and nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-NBP2-49244-img0001.jpg)
![Immunohistochemistry-Paraffin: SAT2 Antibody [NBP2-49244] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP2-49244-img0002.jpg)
![Immunohistochemistry-Paraffin: SAT2 Antibody [NBP2-49244] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP2-49244-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SAT2 Antibody [NBP2-49244] - Staining in human adrenal gland and pancreas tissues using anti-SAT2 antibody. Corresponding SAT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP2-49244-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SAT2 Antibody [NBP2-49244] - Staining of human adrenal gland, colon, pancreas and small intestine using Anti-SAT2 antibody NBP2-49244 (A) shows similar protein distribution across tissues to independent antibody NBP1-80722 (B).](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP2-49244-img0005.jpg)
![Immunohistochemistry-Paraffin: SAT2 Antibody [NBP2-49244] - Staining of human small intestine.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP2-49244-img0007.jpg)
![Immunohistochemistry: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32496_rabbit-ketohexokinase-mab-psh03-18-29820241420952.jpg "Immunohistochemistry: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Immunocytochemistry analysis of HepG2 cells labeling Ketohexokinase with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32496_rabbit-ketohexokinase-mab-psh03-18-2920241192372.jpg)
![Flow Cytometry: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Flow cytometric analysis of HepG2 cells labeling Ketohexokinase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32496, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32496_rabbit-ketohexokinase-mab-psh03-18-39202488461.jpg)
![Western Blot: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Western blot analysis of Ketohexokinase on different lysates with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/2,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Human kidney tissue lysate Lane 3: Mouse liver tissue lysate Lane 4: Mouse kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32496_rabbit-ketohexokinase-mab-psh03-18-392024845350.jpg)