| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38312-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2698-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00064-50ul | |||
![Immunocytochemistry/ Immunofluorescence: Phosphoribosyl Pyrophosphate Amidotransferase Antibody [NBP3-38312] - Immunofluorescence analysis of HeLa cells using Phosphoribosyl Pyrophosphate Amidotransferase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38312_rabbit-phosphoribosyl-pyrophosphate-amidotransferase-pab-1012202411535858.jpg "Immunocytochemistry/ Immunofluorescence: Phosphoribosyl Pyrophosphate Amidotransferase Antibody [NBP3-38312] - Immunofluorescence analysis of HeLa cells using Phosphoribosyl Pyrophosphate Amidotransferase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody [NBP3-38312] - Western blot analysis of lysates from Rat spleen, using Phosphoribosyl Pyrophosphate Amidotransferase Rabbit pAb at 1:2000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38312_rabbit-phosphoribosyl-pyrophosphate-amidotransferase-pab-1012202411552853.jpg)
![Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of Rat kidney, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/GCLC-Antibody-0X1H3-Western-Blot-NBP3-16422-img0002.jpg "Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of Rat kidney, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: GCLC Antibody (0X1H3) [NBP3-16422] - Rat kidney using GCLC Rabbit mAb (NBP3-16422) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GCLC-Antibody-0X1H3-Immunohistochemistry-Paraffin-NBP3-16422-img0001.jpg)
![Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of various cell lines, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/GCLC-Antibody-0X1H3-Western-Blot-NBP3-16422-img0003.jpg)
![Western Blot: Renalase Antibody (1C11E8) [NBP2-52554] - Analysis using RNLS mAb against human RNLS (AA: 68-242) recombinant protein. (Expected MW is 45.7 kDa)](http://images.novusbio.com/fullsize/Renalase-Antibody-1C11E8-Western-Blot-NBP2-52554-img0001.jpg "Western Blot: Renalase Antibody (1C11E8) [NBP2-52554] - Analysis using RNLS mAb against human RNLS (AA: 68-242) recombinant protein. (Expected MW is 45.7 kDa)")
![Western Blot: Renalase Antibody (1C11E8) [NBP2-52554] - Analysis using RNLS mAb against HEK293 (1) and RNLS (AA: 68-242)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Renalase-Antibody-1C11E8-Western-Blot-NBP2-52554-img0002.jpg)
![ELISA: Renalase Antibody (1C11E8) [NBP2-52554] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Renalase-Antibody-1C11E8-ELISA-NBP2-52554-img0003.jpg)
![Immunohistochemistry: TIGAR/C12orf5 Antibody (M2-P4H2) [NB100-2698] - Formalin-fixed,paraffin-embedded human colorectal cancer tissue using TIGAR/C12orf5 Antibody (M2-P4H2), showing strong cytoplasmic and nuclear staining.](http://images.novusbio.com/fullsize/TIGAR-C12orf5-Antibody-M2-P4H2-Immunohistochemistry-NB100-2698-img0002.jpg "Immunohistochemistry: TIGAR/C12orf5 Antibody (M2-P4H2) [NB100-2698] - Formalin-fixed,paraffin-embedded human colorectal cancer tissue using TIGAR/C12orf5 Antibody (M2-P4H2), showing strong cytoplasmic and nuclear staining.")
![Immunohistochemistry: TIGAR/C12orf5 Antibody (M2-P4H2) [NB100-2698] - Antibody staining of colon cancer tissue.](http://images.novusbio.com/fullsize/TIGAR-C12orf5-Antibody-M2-P4H2-Immunohistochemistry-NB100-2698-img0001.jpg)
![Western Blot: PGC1 alpha Antibody (1G8) [NBP2-37562] - Analysis using PGC1 alpha Antibody (1G8) against HEK293 (1) and PGC-1alpha-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PGC1-alpha-Antibody-1G8-Western-Blot-NBP2-37562-img0003.jpg "Western Blot: PGC1 alpha Antibody (1G8) [NBP2-37562] - Analysis using PGC1 alpha Antibody (1G8) against HEK293 (1) and PGC-1alpha-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: PGC1 alpha Antibody (1G8) [NBP2-37562] - Analysis using PGC1 alpha Antibody (1G8) against human PGC-1alpha (AA: 570-798) recombinant protein. Expected molecular weight is 70 KDa.](http://images.novusbio.com/fullsize/PGC1-alpha-Antibody-1G8-Western-Blot-NBP2-37562-img0002.jpg)
![ELISA: PGC1 alpha Antibody (1G8) [NBP2-37562] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/PGC1-alpha-Antibody-1G8-ELISA-NBP2-37562-img0001.jpg)
![Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM11) [NB600-1024] - Analysis using the Biotin conjugate of NB600-1024. Nitrotyrosine in human liver of severely obesed patients. Staining of paraffin tissue section with clone HM.11. Anti-nitrotyrosine at 2 ug/ml (1h, RT).](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-HM11-Immunohistochemistry-Paraffin-NB600-1024-img0003.jpg "Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM11) [NB600-1024] - Analysis using the Biotin conjugate of NB600-1024. Nitrotyrosine in human liver of severely obesed patients. Staining of paraffin tissue section with clone HM.11. Anti-nitrotyrosine at 2 ug/ml (1h, RT).")
![Western Blot: Cytochrome P450 1A2 Antibody (JE39-22) [NBP3-32241] - Western blot analysis of Cytochrome P450 1A2 on HepG2 cell lysates with Rabbit anti-Cytochrome P450 1A2 antibody (NBP3-32241) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 58 kDa<br/> Observed band size: 50 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32241) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32241_rabbit-cytochrome-p450-1a2-mab-je39-22-278202415295537.jpg "Western Blot: Cytochrome P450 1A2 Antibody (JE39-22) [NBP3-32241] - Western blot analysis of Cytochrome P450 1A2 on HepG2 cell lysates with Rabbit anti-Cytochrome P450 1A2 antibody (NBP3-32241) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 58 kDa<br/> Observed band size: 50 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32241) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Cytochrome P450 1A2 Antibody (JE39-22) [NBP3-32241] - Immunocytochemistry analysis of HepG2 cells labeling Cytochrome P450 1A2 with Rabbit anti-Cytochrome P450 1A2 antibody (NBP3-32241) at 1/250 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytochrome P450 1A2 antibody (NBP3-32241) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32241_rabbit-cytochrome-p450-1a2-mab-je39-22-2920241192349.jpg)
![Flow Cytometry: Cytochrome P450 1A2 Antibody (JE39-22) [NBP3-32241] - Flow cytometric analysis of HepG2 cells labeling Cytochrome P450 1A2.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32241, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32241_rabbit-cytochrome-p450-1a2-mab-je39-22-39202488467.jpg)
![Western Blot: Glutathione Peroxidase 3/GPX3 Antibody [NBP2-82233] - WB Suggested Anti-GPX3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysateGPX3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Western-Blot-NBP2-82233-img0002.jpg "Western Blot: Glutathione Peroxidase 3/GPX3 Antibody [NBP2-82233] - WB Suggested Anti-GPX3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysateGPX3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells")
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 3/GPX3 Antibody [NBP2-82233] - Rabbit Anti-GPX3 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue. Observed Staining: Membrane and cytoplasmic in alveolar type I & II cells. Primary Antibody Concentration: 1:100. Other Working Concentrations: 1/600. Secondary Antibod](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Immunohistochemistry-Paraffin-NBP2-82233-img0001.jpg)
![Immunohistochemistry: LAP3 Antibody (PSH01-34) [NBP3-32526] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LAP3 antibody (NBP3-32526) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32526) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32526_rabbit-lap3-mab-psh01-34-298202414333666.jpg "Immunohistochemistry: LAP3 Antibody (PSH01-34) [NBP3-32526] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LAP3 antibody (NBP3-32526) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32526) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32526 at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HEK-293 cell lysate (20 µg/Lane) Lane 4: U-87 MG cell lysate (20 µg/Lane) Lane 5: K-562 cell lysate (20 µg/Lane) Lane 6: A549 cell lysate (20 µg/Lane) Lane 7: LO2 cell lysate (20 µg/Lane) Lane 8: Mouse liver tissue lysate (40 µg/Lane) Lane 9: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32526 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32526_-western-blot-639204595242138461.jpg)
![Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody at 1:500 dilution. Lane A: ADP-Sugar Pyrophosphatase/NUDT5 knockout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/ADP-Sugar-Pyrophosphatase-NUDT5-Antibody-Western-Blot-NBP3-00064-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody at 1:500 dilution. Lane A: ADP-Sugar Pyrophosphatase/NUDT5 knockout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa")
![<b>Genetic Strategies Validation. </b>Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/ADP-Sugar-Pyrophosphatase-NUDT5-Antibody-Western-Blot-NBP3-00064-img0006.jpg)
![Immunoprecipitation: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - ADP-Sugar Pyrophosphatase/NUDT5 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 2 ul anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/ADP-Sugar-Pyrophosphatase-NUDT5-Antibody-Immunoprecipitation-NBP3-00064-img0004.jpg)
![Immunohistochemistry: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32325) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32325_rabbit-fabp3-h-fabp-mab-je53-37-29820241435241.jpg "Immunohistochemistry: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32325) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32325 at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32325 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32325_-western-blot-639204597142175902.jpg)
![Immunohistochemistry: PADI4 Antibody (PSH01-33) [NBP3-32707] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PADI4 antibody (NBP3-32707) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32707) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32707_rabbit-padi4-mab-psh01-33-298202414161870.jpg "Immunohistochemistry: PADI4 Antibody (PSH01-33) [NBP3-32707] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PADI4 antibody (NBP3-32707) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32707) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32707 at 1/1,000 dilution. Lane 1: 293T overexpress PADI4 whole cell lysate Lane 2: 293T whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32707 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32707_-western-blot-639204595241894134.jpg)