Nitrotyrosine Antibody (HM.11) - Azide Free

Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM11) [NB600-1024] - Analysis using the Biotin conjugate of NB600-1024. Nitrotyrosine in human liver of severely obesed patients. Staining of paraffin tissue section with clone HM.11. Anti-nitrotyrosine at 2 ug/ml (1h, RT).

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: HM.11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free
• Concentration: 0.1 mg/ml

Nitrotyrosine Antibody (HM.11) - Azide Free Summary

• Description: Novus Biologicals Mouse Nitrotyrosine Antibody (HM.11) - Azide Free (NB600-1024) is a monoclonal antibody validated for use in IHC and WB. Anti-Nitrotyrosine Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Nitrated KLH
• Specificity: This highly specific monoclonal antibody HM.11 reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Nitrotyrosine is a stable end product of nitrosylation of tyrosine and can be detected by histology on paraffin sections and frozen sections and by Western blot. The antibody is suitable for ELISA applications. (Warning: keep sterile, do not add sodium azide or any nitric containing preservative since this will affect the antibody)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS and 0.1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Nitrotyrosine Antibody (HM.11) - Azide Free

• Nitrotyrosine

Background

The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nitrotyrosine Antibody (NB600-1024)(1)

Publication using NB600-1024

• Abu-Lubad M, Meyer TF, Al-Zeer MA. Chlamydia trachomatis Inhibits Inducible NO Synthase in Human Mesenchymal Stem Cells by Stimulating Polyamine Synthesis J. Immunol. 2014-08-11 [PMID: 25114102]