| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05154-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50387-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-23518-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90076-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81108-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26154-50ul | |||
![Western Blot: Aconitase 2 Overexpression Lysate (Adult Normal) [NBL1-07244] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aconitase 2.](http://images.novusbio.com/fullsize/Aconitase-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07244-img0002.jpg "Western Blot: Aconitase 2 Overexpression Lysate (Adult Normal) [NBL1-07244] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aconitase 2.")
![Western Blot: Aconitase 2 Overexpression Lysate (Adult Normal) [NBL1-07244] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for ACO2](http://images.novusbio.com/fullsize/Aconitase-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07244-img0001.jpg)
![Western Blot: MAP4K3 Antibody [NBP3-05154] - Analysis of extracts of various cell lines, using MAP4K3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/MAP4K3-Antibody-Western-Blot-NBP3-05154-img0002.jpg "Western Blot: MAP4K3 Antibody [NBP3-05154] - Analysis of extracts of various cell lines, using MAP4K3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Western Blot: PLA2G2A Antibody (3X9F5) [NBP3-15715] - Western blot analysis of extracts of various cell lines, using PLA2G2A antibody (NBP3-15715) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/PLA2G2A-Antibody-3X9F5-Western-Blot-NBP3-15715-img0001.jpg "Western Blot: PLA2G2A Antibody (3X9F5) [NBP3-15715] - Western blot analysis of extracts of various cell lines, using PLA2G2A antibody (NBP3-15715) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with IGF1 (100ng/ml, 15min;+) (line B); treated with IGF1 and -phosphatase (line C) using NBP3-23518 at 1:5000 dilution.](http://images.novusbio.com/fullsize/nbp3-23518_rabbit-4ebp1-mab-0014-azide-and-bsa-free-235202511545617.png "Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with IGF1 (100ng/ml, 15min;+) (line B); treated with IGF1 and -phosphatase (line C) using NBP3-23518 at 1:5000 dilution.")
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from HEK293, untreated(line A) or treated with IGF1 (100ng/ml, 15min; +)(line B), using NBP3-23518 at 1:5000 dilution (upper) or Mouse Monoclonal 4EBP1 Antibody at 1:5000 dilution (lower).](http://images.novusbio.com/fullsize/nbp3-23518_rabbit-4ebp1-mab-0014-azide-and-bsa-free-23520251628169.png)
![Immunohistochemistry-Paraffin: CD98 Antibody (071) [NBP2-90076] - Staining of human SLC3A2 in human placenta with rabbit monoclonal antibody (1:500).](http://images.novusbio.com/fullsize/CD98-Antibody-071-Immunohistochemistry-Paraffin-NBP2-90076-img0002.jpg "Immunohistochemistry-Paraffin: CD98 Antibody (071) [NBP2-90076] - Staining of human SLC3A2 in human placenta with rabbit monoclonal antibody (1:500).")
![Immunohistochemistry-Paraffin: CD98 Antibody (071) [NBP2-90076] - Staining of human SLC3A2 in human kidney with rabbit monoclonal antibody (1:500).](http://images.novusbio.com/fullsize/CD98-Antibody-071-Immunohistochemistry-Paraffin-NBP2-90076-img0001.jpg)
![Western Blot: GRP78/HSPA5 Antibody (A5-C9-G6-R) [NBP3-32409] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32409) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 72 kDa Observed band size: 70/72 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32409) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32409_mouse-grp78-hspa5-mab-a5-c9-g6-r-278202415242693.jpg "Western Blot: GRP78/HSPA5 Antibody (A5-C9-G6-R) [NBP3-32409] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32409) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 72 kDa Observed band size: 70/72 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32409) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32790_rabbit-pras40-p-thr246-mab-je53-94-288202412442948.jpg "Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry /Immunofluorescence: CD98 Antibody (HBJ127) - Azide and BSA Free [NBP2-81108] - Immunofluorescence staining of fixed HeLa cells with [NBP2-81108]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with NBP2-81108 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81108, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81108_mouse-monoclonal-cd98-antibody-hbj127-7420252214123.png "Immunocytochemistry /Immunofluorescence: CD98 Antibody (HBJ127) - Azide and BSA Free [NBP2-81108] - Immunofluorescence staining of fixed HeLa cells with [NBP2-81108]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with NBP2-81108 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81108, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.")
![SDS-Page: Recombinant Human GRP78/HSPA5 His Protein [NBP3-18319] - SDS-PAGE of 78kDa Grp78 (Bip) protein.](http://images.novusbio.com/fullsize/Recombinant-Human-GRP78-HSPA5-His-Protein-SDS-Page-NBP3-18319-img0001.jpg "SDS-Page: Recombinant Human GRP78/HSPA5 His Protein [NBP3-18319] - SDS-PAGE of 78kDa Grp78 (Bip) protein.")
![Western Blot: LC3B Antibody (7D11) [NBP3-26154] - Positive Western Blot detected in: HepG2 whole cell lysate, U87 whole cell lysate, RAW264.7 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate. All lanes: LC3B Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 kDa Observed band size: 15 kDa](http://images.novusbio.com/fullsize/nbp3-26154_rabbit-lc3b-mab-7d11-28220241144268.jpg "Western Blot: LC3B Antibody (7D11) [NBP3-26154] - Positive Western Blot detected in: HepG2 whole cell lysate, U87 whole cell lysate, RAW264.7 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate. All lanes: LC3B Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 kDa Observed band size: 15 kDa")
![Flow Cytometry: LC3B Antibody (7D11) [NBP3-26154] - Overlay Peak curve showing Hela cells stained with LC3B Antibody (7D11) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26154_rabbit-lc3b-mab-7d11-282202414183621.jpg)