| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59692-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59338-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90677-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90271-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89518-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26698-50ul | |||
![Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of Rat kidney, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/GCLC-Antibody-0X1H3-Western-Blot-NBP3-16422-img0002.jpg "Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of Rat kidney, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: GCLC Antibody (0X1H3) [NBP3-16422] - Rat kidney using GCLC Rabbit mAb (NBP3-16422) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GCLC-Antibody-0X1H3-Immunohistochemistry-Paraffin-NBP3-16422-img0001.jpg)
![Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of various cell lines, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/GCLC-Antibody-0X1H3-Western-Blot-NBP3-16422-img0003.jpg)
![Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-3C5-1A4-Western-Blot-NBP2-59692-img0002.jpg "Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Mouse fibroblast cell line (NIH 3T3). Fixed in 4% Formaldehyde for 15 min at RT. Primary antibody at 1:100 for 60 min at RT. Secondary antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstaied with DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-3C5-1A4-Immunocytochemistry-Immunofluorescence-NBP2-59692-img0003.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338] - Analysis of Human, Mouse, Rat Rat Kidney Lysate showing detection of ~31 kDa HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 6B8-2F2. Lane 1: MW Ladder. Lane 2: Rat Kidney Lysate. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~31 kDa.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-6B8-2F2-Western-Blot-NBP2-59338-img0004.jpg "Western Blot: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338] - Analysis of Human, Mouse, Rat Rat Kidney Lysate showing detection of ~31 kDa HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 6B8-2F2. Lane 1: MW Ladder. Lane 2: Rat Kidney Lysate. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~31 kDa.")
![Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1/HMOX1/HSP32 (Rat) Monoclonal Antibody, Clone 6B8-2F2 (NBP2-59338). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HO-1/HMOX1/HSP32 (Rat) Monoclonal Antibody (NBP2-59338) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-6B8-2F2-Immunocytochemistry-Immunofluorescence-NBP2-59338-img0005.jpg)
![Western Blot: LRP2 Antibody (PSH02-91) [NBP3-32545] - Western blot analysis of LRP2 on human kidney tissue lysates with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32545) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32545_rabbit-lrp2-mab-psh02-91-278202416285222.jpg "Western Blot: LRP2 Antibody (PSH02-91) [NBP3-32545] - Western blot analysis of LRP2 on human kidney tissue lysates with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32545) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LRP2 Antibody (PSH02-91) [NBP3-32545] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling LRP2 with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32545, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32545_rabbit-lrp2-mab-psh02-91-29820241492681.jpg)
![Immunohistochemistry: LRP2 Antibody (PSH02-91) [NBP3-32545] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32545) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32545_rabbit-lrp2-mab-psh02-91-29820241492624.jpg)
![Western Blot: LRP2 Antibody (PSH04-52) [NBP3-32546] - Western blot analysis of LRP2 on different lysates with Rabbit anti-LRP2 antibody (NBP3-32546) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat kidney tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32546) at 1/2,000 dilution was used in TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32546_rabbit-lrp2-mab-psh04-52-27820241628527.jpg "Western Blot: LRP2 Antibody (PSH04-52) [NBP3-32546] - Western blot analysis of LRP2 on different lysates with Rabbit anti-LRP2 antibody (NBP3-32546) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat kidney tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32546) at 1/2,000 dilution was used in TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LRP2 Antibody (PSH04-52) [NBP3-32546] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LRP2 antibody (NBP3-32546) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32546) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32546_rabbit-lrp2-mab-psh04-52-298202414262677.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0004.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse stomach with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0001.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0002.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0002.jpg "Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunofluorescence staining of AIF-1/Iba1 in K562 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90271 (dilution ratio 1:60) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.](http://images.novusbio.com/fullsize/nbp2-90271_rabbit-monoclonal-aif-1-iba1-antibody-031-235202514492357.png)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518] - Staining of human CA4 in human kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to renal tubules.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IV-CA4-Antibody-039-Immunohistochemistry-Paraffin-NBP2-89518-img0002.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518] - Staining of human CA4 in human kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to renal tubules.")
![Flow Cytometry: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518] - Human primary renal tubular epithelial cells stained with carbonic Anhydrase IV/CA4 antibody conjugated to Alexa Fluor 488 (NBP2-89518AF488). Cells were immediately fixed with 4% PFA after detachment from culture plates and prior to staining. Final Ab concentration was 1:50. Image from verified customer review.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IV-CA4-Antibody-039-Flow-Cytometry-NBP2-89518-img0003.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518] - Staining of human CA4 in human lung with rabbit monoclonal antibody (1:200). Positive staining was localized to pulmonary capillaries.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IV-CA4-Antibody-039-Immunohistochemistry-Paraffin-NBP2-89518-img0001.jpg)
![Western Blot: iNOS [p Tyr1055] Antibody [NBP3-23217] - Western blot analysis of mouse macrophages (J774A.1) treated with LPS (1ug/ml) for 18 hrs followed by pervanadate (1 mM) for 30 min. (lanes 1, 3 & 5). The blots were then treated with akaline phosphatase (lanes 2, 4 & 6). Blots were probed with rabbit polyclonal anti-inducible Nitric Oxide Synthase (iNOS) (lanes 1 & 2), anti-iNOS (Tyr-1055) (lanes 3 & 4), and mouse monoclonal anti-iNOS (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23217_rabbit-inos-p-tyr1055-pab-19920231759440.jpg "Western Blot: iNOS [p Tyr1055] Antibody [NBP3-23217] - Western blot analysis of mouse macrophages (J774A.1) treated with LPS (1ug/ml) for 18 hrs followed by pervanadate (1 mM) for 30 min. (lanes 1, 3 & 5). The blots were then treated with akaline phosphatase (lanes 2, 4 & 6). Blots were probed with rabbit polyclonal anti-inducible Nitric Oxide Synthase (iNOS) (lanes 1 & 2), anti-iNOS (Tyr-1055) (lanes 3 & 4), and mouse monoclonal anti-iNOS (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: iNOS [p Tyr1055] Antibody [NBP3-23217] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23217_rabbit-inos-p-tyr1055-pab-19920231992812.jpg)
![Immunohistochemistry: Caspase-3 Antibody (14C1) [NBP3-26698] - Image of Caspase-3 Antibody (14C1) diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.](http://images.novusbio.com/fullsize/nbp3-26698_rabbit-caspase-3-mab-14c1-262202414471523.jpg "Immunohistochemistry: Caspase-3 Antibody (14C1) [NBP3-26698] - Image of Caspase-3 Antibody (14C1) diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.")
![Immunohistochemistry: Caspase-3 Antibody (14C1) [NBP3-26698] - Image of Caspase-3 Antibody (14C1) diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.](http://images.novusbio.com/fullsize/nbp3-26698_rabbit-caspase-3-mab-14c1-26220241447154.jpg)
![Western Blot: Caspase-3 Antibody (14C1) [NBP3-26698] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate. All lanes: Caspase-3 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa Observed band size: 32 kDa](http://images.novusbio.com/fullsize/nbp3-26698_rabbit-caspase-3-mab-14c1-282202411442633.jpg)