| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26497-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37921-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32052-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38285-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47693-0.1mg | |||
| IHC-Prediluted | NBP2-48163 | |||
![Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-288202412461658.jpg "Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32834) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-298202414333688.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunocytochemistry analysis of HepG2 cells labeling PMP70 with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-29202410334056.jpg)
![Flow Cytometry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Flow cytometric analysis of NIH/3T3 cells labeling PMP70. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32834, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-2920241637212.jpg)
![Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-278202416285288.jpg "Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CLPP Antibody (JE40-00) [NBP3-32195] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29820241492667.jpg)
![Immunocytochemistry/ Immunofluorescence: CLPP Antibody (JE40-00) [NBP3-32195] - Immunocytochemistry analysis of HeLa cells labeling CLPP with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202410334080.jpg)
![Flow Cytometry: CLPP Antibody (JE40-00) [NBP3-32195] - Flow cytometric analysis of HeLa cells labeling CLPP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32195, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202416372131.jpg)
![Western Blot:SCAP Antibody [NBP2-04113] -Whole cell lysate (10 ug) from HEK293T, Hep-G2, HeLa,Jurkat, and K-562 cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-SCAP antibody used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/antibody/nbp2-04113_rabbit-polyclonal-scap-antibody-western-blot-2392024124054..jpg "Western Blot:SCAP Antibody [NBP2-04113] -Whole cell lysate (10 ug) from HEK293T, Hep-G2, HeLa,Jurkat, and K-562 cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-SCAP antibody used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:SCAP Antibody [NBP2-04113] -Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HEK293T cellsprepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-SCAP antibody usedfor IP at 6 µg per reaction. SCAP was alsoimmunoprecipitated by a previous lot of this antibodyFor blotting immunoprecipitated SCAP,was used at 0.1 µg/ml. Detection:Chemiluminescence with an exposure time of 30 seconds](http://images.novusbio.com/fullsize/antibody/nbp2-04113_rabbit-polyclonal-scap-antibody-immunoprecipitation-2392024131242..jpg)
![Immunoprecipitation: ATF4 Antibody (9E1) [NBP3-26497] - Immunoprecipitating ATF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26497 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26497 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-26220241322155.jpg "Immunoprecipitation: ATF4 Antibody (9E1) [NBP3-26497] - Immunoprecipitating ATF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26497 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26497 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).")
![Immunohistochemistry: ATF4 Antibody (9E1) [NBP3-26497] - Image of ATF4 Antibody (9E1) diluted at 1:160 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-26220241510616.jpg)
![Western Blot: ATF4 Antibody (9E1) [NBP3-26497] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate. All lanes: ATF4 Antibody at 1.6ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 KDa Observed band size: 50 KDa](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-282202411472134.jpg)
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (9E1) [NBP3-26497] - Staining of HepG2 cells with ATF4 Antibody (9E1) at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-28220241312211.jpg)
![Flow Cytometry: ATF4 Antibody (9E1) [NBP3-26497] - Overlay histogram showing Hela cells stained with ATF4 Antibody (9E1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-282202414312786.jpg)
![Western Blot: 4EBP1 Antibody [NBP2-16307] - Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP2-16307-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 Antibody [NBP2-16307] - Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 Antibody [NBP2-16307] - 4EBP1 antibody detects 4EBP1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 antibody diluted at a dilution of 1:2000.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP2-16307-img0006.jpg)
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP2-16307] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/eIF4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16307-img0003.jpg)
![Immunoprecipitation: 4EBP1 Antibody [NBP2-16307] - Immunoprecipitation of 4EBP1 protein from HCT116 whole cell extracts using 5 ug of 4EBP1 antibody or 4E-BP1 antibody. Western blot analysis was performed using 4EBP1 antibody.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunoprecipitation-NBP2-16307-img0005.jpg)
![Immunohistochemistry-Paraffin: SCAP Antibody [NBP2-15127] - Mouse kidney. SREBF chaperone protein antibody [C2C3], C-term dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SCAP-Antibody-Immunohistochemistry-Paraffin-NBP2-15127-img0007.jpg "Immunohistochemistry-Paraffin: SCAP Antibody [NBP2-15127] - Mouse kidney. SREBF chaperone protein antibody [C2C3], C-term dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: SCAP Antibody [NBP2-15127] - (30 ug of whole cell lysate) Hep G2 5% SDS PAGE, antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/SCAP-Antibody-Western-Blot-NBP2-15127-img0001.jpg)
![Immunohistochemistry-Paraffin: SCAP Antibody [NBP2-15127] - Mouse kidney. SREBF chaperone protein antibody [C2C3], C-term dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SCAP-Antibody-Immunohistochemistry-Paraffin-NBP2-15127-img0006.jpg)
![Western Blot: SCAP Antibody [NBP2-15127] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SCAP antibody [C2C3], C-term (NBP2-15127) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15127_rabbit-polyclonal-scap-antibody-91020242041229.jpg)
![Western Blot: EDEM2 Antibody [NBP2-37921] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma](http://images.novusbio.com/fullsize/EDEM2-Antibody-Western-Blot-NBP2-37921-img0002.jpg "Western Blot: EDEM2 Antibody [NBP2-37921] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma")
![Immunohistochemistry-Paraffin: EDEM2 Antibody [NBP2-37921] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EDEM2-Antibody-Immunohistochemistry-Paraffin-NBP2-37921-img0007.jpg)
![Immunohistochemistry-Paraffin: EDEM2 Antibody [NBP2-37921] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EDEM2-Antibody-Immunohistochemistry-Paraffin-NBP2-37921-img0003.jpg)
![Immunohistochemistry-Paraffin: EDEM2 Antibody [NBP2-37921] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/EDEM2-Antibody-Immunohistochemistry-Paraffin-NBP2-37921-img0004.jpg)
![Immunohistochemistry-Paraffin: EDEM2 Antibody [NBP2-37921] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/EDEM2-Antibody-Immunohistochemistry-Paraffin-NBP2-37921-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT Antibody [NBP2-32052] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/ACAT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32052-img0008.jpg "Immunocytochemistry/Immunofluorescence: ACAT Antibody [NBP2-32052] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACAT Antibody [NBP2-32052] - Staining in human adrenal gland and cerebral cortex tissues using anti-SOAT1 antibody. Corresponding SOAT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACAT-Antibody-Immunohistochemistry-Paraffin-NBP2-32052-img0012.jpg)
![Immunohistochemistry: ACAT Antibody [NBP2-32052] - Staining of liver.](http://images.novusbio.com/fullsize/ACAT-Antibody-Immunohistochemistry-NBP2-32052-img0003.jpg)
![Immunohistochemistry: ACAT Antibody [NBP2-32052] - Staining of liver cancer.](http://images.novusbio.com/fullsize/ACAT-Antibody-Immunohistochemistry-NBP2-32052-img0004.jpg)
![Immunohistochemistry-Paraffin: ACAT Antibody [NBP2-32052] - Staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.](http://images.novusbio.com/fullsize/ACAT-Antibody-Immunohistochemistry-Paraffin-NBP2-32052-img0010.jpg)
![Immunohistochemistry-Paraffin: ACAT Antibody [NBP2-32052] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/ACAT-Antibody-Immunohistochemistry-Paraffin-NBP2-32052-img0011.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining in human parathyroid gland and pancreas tissues using anti-IMMT antibody. Corresponding IMMT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38285-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining in human parathyroid gland and pancreas tissues using anti-IMMT antibody. Corresponding IMMT RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Mitofilin Antibody [NBP2-38285] - Analysis using Anti-IMMT antibody NBP2-38285 (A) shows similar pattern to independent antibody NBP2-38286 (B).](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NBP2-38285-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NBP2-38285] - Immunofluorescent staining of human cell line HEK 293 shows localization to mitochondria.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38285-img0004.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38285-img0005.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38285-img0006.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Wild-type (WT) and 4EBP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13170-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Wild-type (WT) and 4EBP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:1000.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13170-img0001.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13170-img0002.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13170-img0003.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 antibody (NBP3-13170) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13170_rabbit-4ebp1-p-thr37-p-thr46-pab-91020242015715.jpg)
![Western Blot: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLC.](http://images.novusbio.com/fullsize/GCLC-Antibody-OTI1A3-Azide-and-BSA-Free-Western-Blot-NBP2-70830-img0002.jpg "Western Blot: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLC.")
![Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GCLC-Antibody-OTI1A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70830-img0004.jpg)
![Western Blot: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/GCLC-Antibody-OTI1A3-Azide-and-BSA-Free-Western-Blot-NBP2-70830-img0001.jpg)
![Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GCLC-Antibody-OTI1A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70830-img0003.jpg)
![Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GCLC-Antibody-OTI1A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70830-img0005.jpg)
![Western Blot: Cytochrome c Antibody (CYCS/1010) [NBP2-44446] - Western Blot Analysis of human heart lysate using Cytochrome c antibody (CYCS/1010).](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-CYCS-1010-Western-Blot-NBP2-44446-img0005.jpg "Western Blot: Cytochrome c Antibody (CYCS/1010) [NBP2-44446] - Western Blot Analysis of human heart lysate using Cytochrome c antibody (CYCS/1010).")
![Immunohistochemistry-Paraffin: Cytochrome c Antibody (CYCS/1010) [NBP2-44446] - Human Pancreas stained with Cytochrome C Monoclonal Antibody (CYCS/1010).](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-CYCS-1010-Immunohistochemistry-Paraffin-NBP2-44446-img0002.jpg)
![Western Blot: Cytochrome c Antibody (CYCS/1010) [NBP2-44446] - Analysis of human Heart Lysate using Cytochrome C Monoclonal Antibody (CYCS/1010).](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-CYCS-1010-Western-Blot-NBP2-44446-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome c Antibody (CYCS/1010) [NBP2-44446] - Human Heart stained with Cytochrome C Monoclonal Antibody (CYCS/1010).](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-CYCS-1010-Immunohistochemistry-Paraffin-NBP2-44446-img0001.jpg)