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![Western Blot: MARCKS [p Ser158] Antibody [NBP3-05422] - Western blot analysis of extracts from 3T3 cells using MARCKS antibody (NBP3-05422). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/MARCKS [p Ser158] Antibody-Western Blot-NBP3-05422-img0002.jpg "Western Blot: MARCKS [p Ser158] Antibody [NBP3-05422] - Western blot analysis of extracts from 3T3 cells using MARCKS antibody (NBP3-05422). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/Immunofluorescence: MARCKS [p Ser158] Antibody [NBP3-05422] - Immunofluorescence analysis of methanol-fixed HeLa cells using MARCKS antibody (NBP3-05422).](http://images.novusbio.com/fullsize/MARCKS-[p-Ser158]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05422-img0001.jpg)
![Western Blot: MARCKS [p Ser162] Antibody [NBP3-05423] - Western blot analysis of extracts from 293 cells, using MARCKS antibody (NBP3-05423). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/MARCKS [p Ser162] Antibody-Western Blot-NBP3-05423-img0002.jpg "Western Blot: MARCKS [p Ser162] Antibody [NBP3-05423] - Western blot analysis of extracts from 293 cells, using MARCKS antibody (NBP3-05423). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/Immunofluorescence: MARCKS [p Ser162] Antibody [NBP3-05423] - Immunofluorescence analysis of methanol-fixed HeLa cells using MARCKS antibody (NBP3-05423).](http://images.novusbio.com/fullsize/MARCKS-[p-Ser162]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05423-img0001.jpg)
![Western Blot: HPRT Antibody (4N2Q6) [NBP3-16702] - Western blot analysis of extracts of various cell lines, using HPRT1 Rabbit mAb (NBP3-16702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/HPRT-Antibody-4N2Q6-Western-Blot-NBP3-16702-img0001.jpg "Western Blot: HPRT Antibody (4N2Q6) [NBP3-16702] - Western blot analysis of extracts of various cell lines, using HPRT1 Rabbit mAb (NBP3-16702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Western Blot: HPRT Antibody (4N2Q6) [NBP3-16702] - Western blot analysis of lysates from wild type(WT) and HPRT knockout (KO) 293T cells, using [KO Validated] HPRT Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-16702_rabbit-hprt-mab-4n2q6-52202511365112.jpg)
![Immunohistochemistry-Paraffin: PDE8A Antibody - BSA Free [NB110-40457] - Analysis of anti-PDE8A antibody with human prostate at concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PDE8A-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB110-40457-img0001.jpg "Immunohistochemistry-Paraffin: PDE8A Antibody - BSA Free [NB110-40457] - Analysis of anti-PDE8A antibody with human prostate at concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP3-14467] - Immunohistochemistry of formalin-fixed, paraffin-embedded human ovary carcinoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Immunohistochemistry-Paraffin-NBP3-14467-img0002.jpg "Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP3-14467] - Immunohistochemistry of formalin-fixed, paraffin-embedded human ovary carcinoma after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP3-14467] - Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate carcinoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Immunohistochemistry-Paraffin-NBP3-14467-img0001.jpg)
![Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP3-14467] - Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/S1P3-EDG-3 Antibody-Immunohistochemistry-Paraffin-NBP3-14467-img0003.jpg)
![Western Blot: P2Y2 Antibody [NBP2-86745] - Host: Rabbit. Target Name: P2RY2. Sample Tissue: Human Jurkat Whole Cell. Antibody Dilution: 3ug/ml](http://images.novusbio.com/fullsize/P2Y2-Antibody-Western-Blot-NBP2-86745-img0002.jpg "Western Blot: P2Y2 Antibody [NBP2-86745] - Host: Rabbit. Target Name: P2RY2. Sample Tissue: Human Jurkat Whole Cell. Antibody Dilution: 3ug/ml")
![Western Blot: P2Y2 Antibody [NBP2-86745] - WB Suggested Anti-P2RY2 Antibody. Titration: 1.0 ug/ml. Positive Control: 721_B Whole CellP2RY2 is supported by BioGPS gene expression data to be expressed in 721_B](http://images.novusbio.com/fullsize/P2Y2-Antibody-Western-Blot-NBP2-86745-img0001.jpg)
![Western Blot: P2Y2 Antibody [NBP2-86745] - Host: Rabbit. Target: P2RY2. Positive control (+): THP-1 (N30). Negative control (-): A549 (N03). Antibody concentration: 5ug/ml](http://images.novusbio.com/fullsize/P2Y2-Antibody-Western-Blot-NBP2-86745-img0003.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705] - Lane A: NCI-H1299 Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:63 kDa Observed band size:70 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-001-Western-Blot-NBP2-89705-img0002.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705] - Lane A: NCI-H1299 Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:63 kDa Observed band size:70 kDa (We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705] - Staining of human NT5E in human spleen with rabbit monoclonal antibody (1:5000).](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89705-img0001.jpg)
![Western Blot: PLC-beta 3 Antibody (JE39-14) [NBP3-32776] - Western blot analysis of PLC-beta 3 on different lysates with Rabbit anti-PLC-beta 3 antibody (NBP3-32776) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: HCT 116 cell lysate Lane 5: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 139 kDa Observed band size: 150 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32776) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32776_rabbit-plc-beta-3-mab-je39-14-288202412442979.jpg "Western Blot: PLC-beta 3 Antibody (JE39-14) [NBP3-32776] - Western blot analysis of PLC-beta 3 on different lysates with Rabbit anti-PLC-beta 3 antibody (NBP3-32776) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: HCT 116 cell lysate Lane 5: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 139 kDa Observed band size: 150 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32776) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PLC-beta 3 Antibody (JE39-14) [NBP3-32776] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-PLC-beta 3 antibody (NBP3-32776) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32776) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32776_rabbit-plc-beta-3-mab-je39-14-298202414262641.jpg)
![Immunocytochemistry/ Immunofluorescence: PTEN [p Ser380] Antibody (JJ08-81) [NBP3-32791] - ICC staining of PTEN in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32791, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32791_rabbit-pten-p-ser380-mab-jj08-81-2920241192374.jpg "Immunocytochemistry/ Immunofluorescence: PTEN [p Ser380] Antibody (JJ08-81) [NBP3-32791] - ICC staining of PTEN in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32791, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).")
![Western Blot: CD38 Antibody (003) [NBP2-89658] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary antibody goat anti-Rabbit IgG H&L (DyLight 800) at 1:10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:50 kDa.](http://images.novusbio.com/fullsize/CD38-Antibody-003-Western-Blot-NBP2-89658-img0001.jpg "Western Blot: CD38 Antibody (003) [NBP2-89658] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary antibody goat anti-Rabbit IgG H&L (DyLight 800) at 1:10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:50 kDa.")
![Immunoprecipitation: CD38 Antibody (003) [NBP2-89658] - Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-CD38 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CD38 rabbit monoclonal antibody at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa Observed band size: 45 kDa.](http://images.novusbio.com/fullsize/CD38-Antibody-003-Immunoprecipitation-NBP2-89658-img0002.jpg)
![Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-MPO421-8B2-Flow-Intracellular-NBP2-41406-img0002.jpg "Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).")
![Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Intracellular staining pattern of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-MPO421-8B2-Flow-Intracellular-NBP2-41406-img0001.jpg)
![Western Blot: Thioredoxin-1 Antibody (116) [NBP2-89458] - Lane A: Hela Whole Cell Lysate Lane B: THP-1 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:12 kDa Observed band size:14 kDa](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-116-Western-Blot-NBP2-89458-img0001.jpg "Western Blot: Thioredoxin-1 Antibody (116) [NBP2-89458] - Lane A: Hela Whole Cell Lysate Lane B: THP-1 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:12 kDa Observed band size:14 kDa")
![Immunoprecipitation: Thioredoxin-1 Antibody (116) [NBP2-89458] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate2 uL anti-TXN rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-TXN rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 13 kDa](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-116-Immunoprecipitation-NBP2-89458-img0002.jpg)