| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38190-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35155-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37347-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22033-50ul | |||
![Western Blot: GIRK2 Antibody [NB100-74575] - Staining of Human Brain (Substantia Nigra) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GIRK2-Antibody-Western-Blot-NB100-74575-img0001.jpg "Western Blot: GIRK2 Antibody [NB100-74575] - Staining of Human Brain (Substantia Nigra) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241252813.jpg "Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human colon using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241233823.jpg)
![Western Blot: Aspartate Aminotransferase Antibody [NBP3-38190] - Western blot analysis of various lysates using Aspartate Aminotransferase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-1012202411592816.jpg)
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-10122024120650.jpg)
![Immunocytochemistry/ Immunofluorescence: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunofluorescence analysis of U2OS cells using Aspartate Aminotransferase Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241243928.jpg)
![Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (4R3L10) [NBP3-16091] - Analysis of various lysates, using AMACR antibody at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16091_rabbit-alpha-methylacyl-coa-racemase-amacr-mab-4r3l10-western-blot-28202392140..jpg "Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (4R3L10) [NBP3-16091] - Analysis of various lysates, using AMACR antibody at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16091_rabbit-alpha-methylacyl-coa-racemase-amacr-mab-7x5z2-52202511361219.jpg)
![Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human prostate cancer tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16091_rabbit-alpha-methylacyl-coa-racemase-amacr-mab-7x5z2-52202511402799.jpg)
![Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16091_rabbit-alpha-methylacyl-coa-racemase-amacr-mab-7x5z2-52202511361234.jpg)
![Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16091_rabbit-alpha-methylacyl-coa-racemase-amacr-mab-7x5z2-52202511411465.jpg)
![Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16091_rabbit-alpha-methylacyl-coa-racemase-amacr-mab-7x5z2-52202511361267.jpg)
![Western Blot: MEKK1 Antibody [NBP3-02962] - Analysis of extracts of various cell lines, using MEKK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/MEKK1-Antibody-Western-Blot-NBP3-02962-img0004.jpg "Western Blot: MEKK1 Antibody [NBP3-02962] - Analysis of extracts of various cell lines, using MEKK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: MEKK1 Antibody [NBP3-02962] - Analysis of L929 cells using MEKK1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MEKK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-02962-img0001.jpg)
![Immunohistochemistry-Paraffin: MEKK1 Antibody [NBP3-02962] - Mouse kidney using MEKK1 antibody (NBP3-02962) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MEKK1-Antibody-Immunohistochemistry-Paraffin-NBP3-02962-img0005.jpg)
![Immunohistochemistry-Paraffin: MEKK1 Antibody [NBP3-02962] - Human liver using MAP3K1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MEKK1-Antibody-Immunohistochemistry-Paraffin-NBP3-02962-img0002.jpg)
![Immunohistochemistry-Paraffin: MEKK1 Antibody [NBP3-02962] - Rat liver using MAP3K1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MEKK1-Antibody-Immunohistochemistry-Paraffin-NBP3-02962-img0003.jpg)
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415133421.jpg "Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415121312.jpg)
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Rat lung using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202414201353.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody [NBP3-35155] - Immunofluorescence analysis of U-2 OS cells using SEC14L2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415175267.jpg)
![Western Blot: SEC14L2 Antibody [NBP3-35155] - Western blot analysis of various lysates using SEC14L2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415152860.jpg)
![Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of C6 cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Western-Blot-NBP3-15666-img0005.jpg "Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of C6 cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of various cell lines, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Western-Blot-NBP3-15666-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts from normal (control) and PTEN knockout (KO) HeLa cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Knockout-Validated-NBP3-15666-img0001.jpg)
![Immunoprecipitation: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of 300ug extracts of NIH/3T3 cells using 3ug PTEN antibody (NBP3-15666). Western blot was performed from the immunoprecipitate using PTEN antibody (NBP3-15666) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Immunoprecipitation-NBP3-15666-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: PTEN Antibody (7O6F4) [PTEN] - Confocal imaging of NIH/3T3 cells using [KO Validated] PTEN Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15666_rabbit-pten-mab-7o6f4-52202517361229.jpg)
![Western Blot: MRP4/ABCC4 Antibody (6A7H3) [NBP2-37347] - Analysis using ABCC4 mAb against HEK293 (1) and ABCC4 (AA: 631-692)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-6A7H3-Western-Blot-NBP2-37347-img0003.jpg "Western Blot: MRP4/ABCC4 Antibody (6A7H3) [NBP2-37347] - Analysis using ABCC4 mAb against HEK293 (1) and ABCC4 (AA: 631-692)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody (6A7H3) [NBP2-37347] - Analysis of esophagus cancer tissues using ABCC4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-6A7H3-Immunohistochemistry-NBP2-37347-img0006.jpg)
![Flow Cytometry: MRP4/ABCC4 Antibody (6A7H3) [NBP2-37347] - Analysis of A549 cells using ABCC4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-6A7H3-Flow-Cytometry-NBP2-37347-img0004.jpg)
![Western Blot: MRP4/ABCC4 Antibody (6A7H3) [NBP2-37347] - Analysis using ABCC4 mAb against human ABCC4 recombinant protein. (Expected MW is 32.4 kDa)](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-6A7H3-Western-Blot-NBP2-37347-img0002.jpg)
![Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody (6A7H3) [NBP2-37347] - Analysis of liver cancer tissues using ABCC4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-6A7H3-Immunohistochemistry-NBP2-37347-img0005.jpg)
![ELISA: MRP4/ABCC4 Antibody (6A7H3) [NBP2-37347] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-6A7H3-ELISA-NBP2-37347-img0001.jpg)
![Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Western-Blot-NBP1-47398-img0011.jpg "Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Immunocytochemistry-Immunofluorescence-NBP1-47398-img0010.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAb.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Immunohistochemistry-Paraffin-NBP1-47398-img0007.jpg)
![Flow Cytometry: NM23-H1 Antibody (4B2) [NBP1-47398] - Flow cytometric analysis of Jurkat cells using NME1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Flow-Cytometry-NBP1-47398-img0009.jpg)
![Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Western-Blot-NBP2-70739-img0002.jpg "Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.")
![Immunocytochemistry/Immunofluorescence: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1.](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70739-img0005.jpg)
![Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of Jurkat cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70739-img0004.jpg)
![Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Western-Blot-NBP2-70739-img0001.jpg)
![Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of Hela cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70739-img0003.jpg)
![Western Blot: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 ug/Lane) Lane 2: PC-3M cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: C6 cell lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/19 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32656) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-278202415212429.jpg "Western Blot: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 ug/Lane) Lane 2: PC-3M cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: C6 cell lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/19 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32656) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32656) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-298202414333679.jpg)
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Immunocytochemistry analysis of MCF7 cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-29202410484818.jpg)
![Flow Cytometry: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Flow cytometric analysis of MCF7 cells labeling NM23-H1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32656, 1μg/mL) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-29202416372150.jpg)
![Western Blot: Lipase A Antibody [NBP2-19360] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP2-19360-img0002.jpg "Western Blot: Lipase A Antibody [NBP2-19360] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Lipase A Antibody [NBP2-19360] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Lipase A antibody [N3C3] (NBP2-19360) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19360_rabbit-polyclonal-lipase-a-antibody-910202420131257.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-19360] -Mouse kidney.LAL stained by LAL antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-19360_rabbit-polyclonal-lipase-a-antibody-immunohistochemistry-paraffin-11112024104132..png)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAL antibody [N3C3] (NBP2-19360) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19360_rabbit-polyclonal-lipase-a-antibody-western-blot-261120251847578.jpg)
![LAL antibody [N3C3] detects LAL protein at lysosome by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: LAL stained by LAL antibody [N3C3] (NBP2-19360) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19360_rabbit-polyclonal-lipase-a-antibody-immunocytochemistry-immunofluorescence-261120251832194.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - Western blot analysis using NBP3-22033 in A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-57202317215160.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - Western blot analysis using NBP3-22033 in A549 cell lysate.")
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: MCF-7. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202414462214.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: SH-SY5Y. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-2342024155511.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: Hep-G2. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202414462212.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: Mouse lung. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202415232515.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: Rat kidney. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202414462210.jpg)