| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00351-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99633-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90677-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90271-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97726-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26193-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26469-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26504-50ul | |||
![Immunoprecipitation: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] - PMM2 was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate 2 uL NBP3-00351 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00351, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 27 kDa Observed band size: 27 kDa](http://images.novusbio.com/fullsize/nbp3-00351_rabbit-polyclonal-pmm2-phosphomannomutase-2-antibody-235202516335042.png "Immunoprecipitation: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] - PMM2 was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate 2 uL NBP3-00351 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00351, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 27 kDa Observed band size: 27 kDa")
![Western Blot: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] - NBP3-00351 at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00351_rabbit-polyclonal-pmm2-phosphomannomutase-2-antibody-235202511545675.png)
![Western Blot: 14-3-3 epsilon Antibody [NBP2-99633] - Anti-14-3-3 epsilon rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: SHSY5Y Whole Cell Lysate. Lane D: SW480 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP2-99633-img0003.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP2-99633] - Anti-14-3-3 epsilon rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: SHSY5Y Whole Cell Lysate. Lane D: SW480 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP2-99633] - 14-3-3 epsilon was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Lane C: 0.5 mg SH-SY5Y Whole Cell Lysate. 4 ul anti-14-3-3 epsilon rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 epsilon rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP2-99633-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP2-99633] - Immunofluorescence staining of 14-3-3 epsilon in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99633 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99633_rabbit-polyclonal-14-3-3-epsilon-antibody-235202511444474.png)
![Western Blot: LRP2 Antibody (PSH02-91) [NBP3-32545] - Western blot analysis of LRP2 on human kidney tissue lysates with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32545) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32545_rabbit-lrp2-mab-psh02-91-278202416285222.jpg "Western Blot: LRP2 Antibody (PSH02-91) [NBP3-32545] - Western blot analysis of LRP2 on human kidney tissue lysates with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32545) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LRP2 Antibody (PSH02-91) [NBP3-32545] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling LRP2 with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32545, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32545_rabbit-lrp2-mab-psh02-91-29820241492681.jpg)
![Immunohistochemistry: LRP2 Antibody (PSH02-91) [NBP3-32545] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32545) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32545_rabbit-lrp2-mab-psh02-91-29820241492624.jpg)
![Western Blot: LRP2 Antibody (PSH04-52) [NBP3-32546] - Western blot analysis of LRP2 on different lysates with Rabbit anti-LRP2 antibody (NBP3-32546) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat kidney tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32546) at 1/2,000 dilution was used in TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32546_rabbit-lrp2-mab-psh04-52-27820241628527.jpg "Western Blot: LRP2 Antibody (PSH04-52) [NBP3-32546] - Western blot analysis of LRP2 on different lysates with Rabbit anti-LRP2 antibody (NBP3-32546) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat kidney tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32546) at 1/2,000 dilution was used in TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LRP2 Antibody (PSH04-52) [NBP3-32546] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LRP2 antibody (NBP3-32546) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32546) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32546_rabbit-lrp2-mab-psh04-52-298202414262677.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0004.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse stomach with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0001.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0002.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0003.jpg)
![Western Blot: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Western blot analysis of FABP3/H-FABP on different lysates with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32325) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32325_rabbit-fabp3-h-fabp-mab-je53-37-278202416285235.jpg "Western Blot: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Western blot analysis of FABP3/H-FABP on different lysates with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32325) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32325) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32325_rabbit-fabp3-h-fabp-mab-je53-37-29820241435241.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0002.jpg "Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunofluorescence staining of AIF-1/Iba1 in K562 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90271 (dilution ratio 1:60) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.](http://images.novusbio.com/fullsize/nbp2-90271_rabbit-monoclonal-aif-1-iba1-antibody-031-235202514492357.png)
![Western Blot: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: U-251 MG Whole Cell LysateLane D: A375 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP2-97726-img0003.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: U-251 MG Whole Cell LysateLane D: A375 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa")
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-YWHAE rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-YWHAE rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size :30 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP2-97726-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP2-97726] - Immunofluorescence staining of 14-3-3 epsilon in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97726 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97726_rabbit-polyclonal-14-3-3-epsilon-antibody-235202511545662.png)
![Immunohistochemistry: Islet-1 Antibody (1A1) [NBP3-26193] - Image of Islet-1 Antibody (1A1) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26193_rabbit-islet-1-mab-1a1-26220241522421.jpg "Immunohistochemistry: Islet-1 Antibody (1A1) [NBP3-26193] - Image of Islet-1 Antibody (1A1) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Islet-1 Antibody (1A1) [NBP3-26193] - Image of Islet-1 Antibody (1A1) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26193_rabbit-islet-1-mab-1a1-262202415475645.jpg)
![Western Blot: Islet-1 Antibody (1A1) [NBP3-26193] - Positive Western Blot detected in: 293T whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate. All lanes: Islet-1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40 kDa Observed band size: 40 kDa](http://images.novusbio.com/fullsize/nbp3-26193_rabbit-islet-1-mab-1a1-28220241152516.jpg)
![Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Image of Carbonic Anhydrase I/CA1 Antibody (26E3) diluted at 1:50 and staining in paraffin-embedded human spleen tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.36% DAB.](http://images.novusbio.com/fullsize/nbp3-26469_rabbit-carbonic-anhydrase-i-ca1-mab-26e3-26220241525339.jpg "Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Image of Carbonic Anhydrase I/CA1 Antibody (26E3) diluted at 1:50 and staining in paraffin-embedded human spleen tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.36% DAB.")
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Positive Western Blot detected in: K562 whole cell lysate, U937 whole cell lysate. All lanes: Carbonic Anhydrase I/CA1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28 kDa Observed band size: 28 kDa](http://images.novusbio.com/fullsize/nbp3-26469_rabbit-carbonic-anhydrase-i-ca1-mab-26e3-282202411485846.jpg)
![Flow Cytometry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Overlay Peak curve showing MCF-7 cells stained with Carbonic Anhydrase I/CA1 Antibody (26E3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26469_rabbit-carbonic-anhydrase-i-ca1-mab-26e3-282202414183617.jpg)
![Immunohistochemistry: Apolipoprotein A-II/ApoA2 Antibody (6H2) [NBP3-26504] - Image of Apolipoprotein A-II/ApoA2 Antibody (6H2) diluted at 1:87.5 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26504_rabbit-apolipoprotein-a-ii-apoa2-mab-6h2-2722024850462.jpg "Immunohistochemistry: Apolipoprotein A-II/ApoA2 Antibody (6H2) [NBP3-26504] - Image of Apolipoprotein A-II/ApoA2 Antibody (6H2) diluted at 1:87.5 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: Apolipoprotein A-II/ApoA2 Antibody (6H2) [NBP3-26504] - Positive Western Blot detected in: 293T whole cell lysate, A549 whole cell lysate. All lanes: Apolipoprotein A-II/ApoA2 Antibody at 0.87ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 12 KDa Observed band size: 12 KDa](http://images.novusbio.com/fullsize/nbp3-26504_rabbit-apolipoprotein-a-ii-apoa2-mab-6h2-28220241147215.jpg)
![Flow Cytometry: Apolipoprotein A-II/ApoA2 Antibody (6H2) [NBP3-26504] - Overlay histogram showing A549 cells stained with Apolipoprotein A-II/ApoA2 Antibody (6H2) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26504_rabbit-apolipoprotein-a-ii-apoa2-mab-6h2-282202414312783.jpg)