| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92984-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59390-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54369-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16013-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87264-25ul | |||
![Western Blot: DGAT1 Antibody [NBP2-92984] - Analysis of extracts of various cell lines, using DGAT1 antibody (NBP2-92984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Western-Blot-NBP2-92984-img0001.jpg "Western Blot: DGAT1 Antibody [NBP2-92984] - Analysis of extracts of various cell lines, using DGAT1 antibody (NBP2-92984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: DGAT1 Antibody [NBP2-92984] - Analysis of extracts of Mouse liver, using DGAT1 antibody (NBP2-92984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/DGAT1 Antibody-Western Blot-NBP2-92984-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody - BSA Free [NBP2-92984] - Immunofluorescence analysis of NIH/3T3 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92984_rabbit-polyclonal-dgat1-antibody-42202514251841.jpg)
![Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody - BSA Free [NBP2-92984] - Immunofluorescence analysis of MCF7 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92984_rabbit-polyclonal-dgat1-antibody-4220251429077.jpg)
![Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody - BSA Free [NBP2-92984] - Immunofluorescence analysis of PC-12 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92984_rabbit-polyclonal-dgat1-antibody-4220251429013.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human testis shows moderate cytoplasmic positivity in a small subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0009.jpg "Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human testis shows moderate cytoplasmic positivity in a small subset of cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0007.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0008.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0010.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody (GT2781) [NBP3-13615] - Perilipin antibody [GT2781] detects Perilipin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse white adipocyte. Perilipin stained by Perilipin antibody [GT2781] (NBP3-13615) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Perilipin-Antibody-GT2781-Immunohistochemistry-Paraffin-NBP3-13615-img0002.jpg "Immunohistochemistry-Paraffin: Perilipin Antibody (GT2781) [NBP3-13615] - Perilipin antibody [GT2781] detects Perilipin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse white adipocyte. Perilipin stained by Perilipin antibody [GT2781] (NBP3-13615) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Perilipin Antibody (GT2781) [NBP3-13615] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin antibody [GT2781] (NBP3-13615) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Perilipin-Antibody-GT2781-Western-Blot-NBP3-13615-img0001.jpg)
![Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin 1 antibody [GT2781] (NBP3-13615) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13615_mouse-perilipin-mab-gt2781-western-blot-2611202518461022.jpg)
![Perilipin 1 antibody [GT2781] detects Perilipin 1 protein at lipid droplet by immunofluorescent analysis.Sample: Mock and differentiated 3T3-L1 cells were fixed in ice-cold MeOH for 5 min.Green: Perilipin 1 stained by Perilipin 1 antibody [GT2781] (NBP3-13615) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13615_mouse-perilipin-mab-gt2781-immunocytochemistry-immunofluorescence-261120251995411.jpg)
![Western Blot: Perilipin Antibody (1K4W3) [NBP3-16480] - Western blot analysis of extracts of various cell lines, using Perilipin Rabbit mAb (NBP3-16480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Perilipin-Antibody-1K4W3-Western-Blot-NBP3-16480-img0005.jpg "Western Blot: Perilipin Antibody (1K4W3) [NBP3-16480] - Western blot analysis of extracts of various cell lines, using Perilipin Rabbit mAb (NBP3-16480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480] - Immunohistochemistry of paraffin-embedded rat brown adipose using Perilipin Rabbit mAb (NBP3-16480) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Perilipin-Antibody-1K4W3-Immunohistochemistry-Paraffin-NBP3-16480-img0001.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480] - Immunohistochemistry of paraffin-embedded mouse brown adipose using Perilipin Rabbit mAb (NBP3-16480) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Perilipin-Antibody-1K4W3-Immunohistochemistry-Paraffin-NBP3-16480-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Perilipin Antibody (1K4W3) [Perilipin] - Confocal imaging of paraffin-embedded Rat brown fat tissue using Perilipin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16480_rabbit-perilipin-mab-1k4w3-52202517352749.jpg)
![Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection syste](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Western-Blot-NBP2-59390-img0004.jpg "Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection syste")
![Immunocytochemistry/Immunofluorescence: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Analysis of ATGL in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of ATGL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of ATGL in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Immunocytochemistry-Immunofluorescence-NBP2-59390-img0002.jpg)
![Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with NBP2-59390 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-AT18E6-Flow-Cytometry-NBP2-59390-img0006.jpg)
![Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Analysis in A431 cell lysates (40ug), resolved by SDS-PAGE.](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Western-Blot-NBP2-59390-img0001.jpg)
![Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Flow cytometry analysis of ATGL in Hep3B cells. The cell was stained with NBP2-59390 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-AT18E6-Flow-Cytometry-NBP2-59390-img0005.jpg)
![Western Blot: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Western blot analysis of extracts of HeLa cells, using Caveolin-1antibody (NBP3-15615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-9I9J8-Western-Blot-NBP3-15615-img0017.jpg "Western Blot: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Western blot analysis of extracts of HeLa cells, using Caveolin-1antibody (NBP3-15615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Human colon using Caveolin-1 Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-immunohistochemistry-paraffin-184202410253..jpg)
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody (9I9J8) [NBP3-15615] -Human lung using Caveolin-1 Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-immunohistochemistry-paraffin-184202410355..jpg)
![Western Blot: Caveolin-1 Antibody (10Y5L2) [Caveolin-1] - Western blot analysis of various lysates, using Caveolin-1 Rabbit mAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-5220251731106.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - ecombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Western-Blot-NBP2-53401-img0001.jpg "Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - ecombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Monoclonal Antibody (ADFP/1365).")
![Flow Cytometry: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - Flow Cytometry of human Perilipin-2/ADFP on PBMC. Green: Isotype Control; Red: Perilipin-2/ADFP Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Flow-Cytometry-NBP2-53401-img0004.jpg)
![Flow Cytometry: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - Flow Cytometry [NBP2-53401] - Flow Cytometry of human Adipophilin on PBMC. Green: Isotype Control; Red: Adipophilin Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Flow-Cytometry-NBP2-53401-img0003.jpg)
![Protein Array: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Perilipin-2/ADFP Mouse Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Protein-Array-NBP2-53401-img0005.jpg)
![Western Blot: OXPAT Antibody [NBP3-16013] - Western blot analysis of extracts of various cell lines, using OXPAT antibody (NBP3-16013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/OXPAT-Antibody-Western-Blot-NBP3-16013-img0002.jpg "Western Blot: OXPAT Antibody [NBP3-16013] - Western blot analysis of extracts of various cell lines, using OXPAT antibody (NBP3-16013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: OXPAT Antibody [NBP3-16013] - Western blot analysis of extracts of HepG2 cells, using OXPAT antibody (NBP3-16013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/OXPAT-Antibody-Western-Blot-NBP3-16013-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: OXPAT Antibody - Azide and BSA Free [NBP3-16013] - Immunofluorescence analysis of HepG2 cells using OXPAT Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16013_rabbit-oxpat-pab-72202512443378.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369] - Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-54369] - recombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Azide-and-BSA-Free-Western-Blot-NBP2-54369-img0002.jpg "Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369] - Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-54369] - recombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Monoclonal Antibody (ADFP/1365).")
![Flow Cytometry: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369] - Flow Cytometry of human Perilipin-2/ADFP on PBMC. Green: Isotype Control; Red: Perilipin-2/ADFP Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54369-img0003.jpg)
![Protein Array: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Perilipin-2/ADFP Mouse Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Azide-and-BSA-Free-Protein-Array-NBP2-54369-img0004.jpg)
![Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling PEDFR/PNPLA2/ATGL with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32004, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-29820241420941.jpg "Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling PEDFR/PNPLA2/ATGL with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32004, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32004) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-298202414262678.jpg)
![Western Blot: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Western blot analysis of PEDFR/PNPLA2/ATGL on different lysates with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/1,000 dilution. Lane 1: SiHa cell lysate (30 ug/Lane) Lane 2: HepG2 cell lysate (30 ug/Lane) Lane 3: HEK-293 cell lysate (30 ug/Lane) Lane 4: A431 cell lysate (30 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32004) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-392024848502.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody [NBP1-87264] - Analysis in human adipose tissue and cervix, uterine tissues using NBP1-87264 antibody. Corresponding Perilipin RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NBP1-87264-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Perilipin Antibody [NBP1-87264] - Analysis in human adipose tissue and cervix, uterine tissues using NBP1-87264 antibody. Corresponding Perilipin RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Perilipin Antibody [NBP1-87264] - Staining of human breast shows high expression.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NBP1-87264-img0004.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody [NBP1-87264] - Staining of human placenta shows low expression as expected.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NBP1-87264-img0005.jpg)