| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54369-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26636-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77556SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47179SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26565-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85210-25ul | |||
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Western blot analysis of Pyruvate Dehydrogenase E1-alpha subunit on different lysates with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HEK-293 cell lysate (20 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32787) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-288202412442923.jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Western blot analysis of Pyruvate Dehydrogenase E1-alpha subunit on different lysates with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HEK-293 cell lysate (20 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32787) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32787) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-298202414115418.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Immunocytochemistry analysis of Jurkat cells treated with or without lambda pp labeling Pyruvate Dehydrogenase E1-alpha subunit with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-2920241114815.jpg)
![Western Blot: GSK-3 beta Antibody (M131) [NBP3-23366] - Western blot analysis of a human recombinant GSK-3 beta N-terminal fragment (lanes 1 & 2) and endogenous GSK-3 beta expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3 beta at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).](http://images.novusbio.com/fullsize/nbp3-23366_mouse-gsk-3-beta-mab-m131-1992023199282.jpg "Western Blot: GSK-3 beta Antibody (M131) [NBP3-23366] - Western blot analysis of a human recombinant GSK-3 beta N-terminal fragment (lanes 1 & 2) and endogenous GSK-3 beta expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3 beta at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).")
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (M131) [NBP3-23366] - Immunocytochemical labeling of GSK-3 beta in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-GSK-3 beta (NBP3-23366) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23366_mouse-gsk-3-beta-mab-m131-19920231252364.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - ecombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Western-Blot-NBP2-53401-img0001.jpg "Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - ecombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Monoclonal Antibody (ADFP/1365).")
![Flow Cytometry: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - Flow Cytometry of human Perilipin-2/ADFP on PBMC. Green: Isotype Control; Red: Perilipin-2/ADFP Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Flow-Cytometry-NBP2-53401-img0004.jpg)
![Flow Cytometry: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - Flow Cytometry [NBP2-53401] - Flow Cytometry of human Adipophilin on PBMC. Green: Isotype Control; Red: Adipophilin Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Flow-Cytometry-NBP2-53401-img0003.jpg)
![Protein Array: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Perilipin-2/ADFP Mouse Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Protein-Array-NBP2-53401-img0005.jpg)
![Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - NBP3-26636 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101148.jpg "Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - NBP3-26636 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody")
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of Hela cell with NBP3-26636 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101147.jpg)
![Western Blot: Aromatase Antibody (1H1) [NBP3-26636] - Positive WB detected in: 293 whole cell lysate (20µg), K562 whole cell lysate (20µg), Hela whole cell lysate (20µg), MCF-7 whole cell lysate (20µg), PC-3 whole cell lysate (20µg)All lanes: CYP19A1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 kDaObserved band size: 58 kDaExposure time：120s](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101143.jpg)
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-26120251011416.jpg)
![Western Blot: Tryptophan hydroxylase 2 Antibody [NBP1-77556] - Analysis of TPHZ in human stomach lysate.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Western-Blot-NBP1-77556-img0001.jpg "Western Blot: Tryptophan hydroxylase 2 Antibody [NBP1-77556] - Analysis of TPHZ in human stomach lysate.")
![Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-10-Antibody-exon-10-Western-Blot-NBP1-47179-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein")
![Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] - Image of FTO Antibody (4G9) diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26565_rabbit-fto-mab-4g9-27220248453212.jpg "Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] - Image of FTO Antibody (4G9) diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] - Image of FTO Antibody (4G9) diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26565_rabbit-fto-mab-4g9-27220248573310.jpg)
![Western Blot: FTO Antibody (4G9) [NBP3-26565] - Positive Western Blot detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate, Raji whole cell lysate, Colo320 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate. All lanes: FTO Antibody at 0.7ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59, 15, 7, 13 KDa Observed band size: 59 KDa](http://images.novusbio.com/fullsize/nbp3-26565_rabbit-fto-mab-4g9-282202411485835.jpg)
![Immunocytochemistry/Immunofluorescence: FTO Antibody (4G9) [NBP3-26565] - Staining of Hela cells with FTO Antibody (4G9) at 1:23, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26565_rabbit-fto-mab-4g9-282202412475115.jpg)
![Western Blot: CD39L2/ENTPD6 Antibody [NBP1-32294] - Sample(30 ug of whole cell lysate)A:H1299 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/CD39L2-ENTPD6-Antibody-Western-Blot-NBP1-32294-img0006.jpg "Western Blot: CD39L2/ENTPD6 Antibody [NBP1-32294] - Sample(30 ug of whole cell lysate)A:H1299 7. 5% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: CD39L2/ENTPD6 Antibody [NBP1-32294] - Mouse heart. ENTPD6 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CD39L2-ENTPD6-Antibody-Immunohistochemistry-Paraffin-NBP1-32294-img0007.jpg)
![Immunohistochemistry-Paraffin: CD39L2/ENTPD6 Antibody [NBP1-32294] - Ca922 xenograft, using ENTPD6 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CD39L2-ENTPD6-Antibody-Immunohistochemistry-Paraffin-NBP1-32294-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Mind Bomb 1/MIB1 Antibody [NBP1-85210] - Staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Mind-Bomb-1-MIB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85210-img0004.jpg "Immunocytochemistry/Immunofluorescence: Mind Bomb 1/MIB1 Antibody [NBP1-85210] - Staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Mind Bomb 1/MIB1 Antibody [NBP1-85210] - Staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Mind-Bomb-1-MIB1-Antibody-Immunohistochemistry-Paraffin-NBP1-85210-img0002.jpg)