| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43458-0.5mg | |||
| BSA Free | NBP1-43458-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65362-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76605-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-12159-0.025mg | |||
![Flow Cytometry: CD3 Antibody (SK7) [NBP3-09140] - Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (SK7) purified antibody (concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD3-Antibody-SK7-Flow-Cytometry-NBP3-09140-img0002.jpg "Flow Cytometry: CD3 Antibody (SK7) [NBP3-09140] - Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (SK7) purified antibody (concentration in sample 4 ug/ml) GAM APC.")
![Flow Cytometry: CD3 Antibody (SK7) [NBP3-09140] - Surface staining pattern of human peripheral whole blood stained using anti-human CD3 (SK7) purified antibody (concentration in sample 4 ug/ml) GAM APC](http://images.novusbio.com/fullsize/CD3-Antibody-SK7-Flow-Cytometry-NBP3-09140-img0001.jpg)
![Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Human lung cancer. HLA-G antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HLA-G-Antibody-Immunohistochemistry-Paraffin-NBP2-16855-img0003.jpg "Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Human lung cancer. HLA-G antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Mahlarvu xenograft, using HLA-G antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/HLA-G-Antibody-Immunohistochemistry-Paraffin-NBP2-16855-img0001.jpg)
![Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Human endometrial cancer. HLA-G antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HLA-G-Antibody-Immunohistochemistry-Paraffin-NBP2-16855-img0002.jpg)
![Immunoprecipitation: STAT6 Antibody [NBP3-14726] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from UACC-62 cells.Antibodies: Rabbit anti-STAT6 recombinant monoclonalantibody used for IP at 20ul/mg lysate. STAT6 was also immunoprecipitated by otherantibodies against different epitopes of STAT6. For blotting immunoprecipitatedSTAT6, was used at 1:1000. Chemiluminescencewith an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunoprecipitation-NBP3-14726-img0001.jpg "Immunoprecipitation: STAT6 Antibody [NBP3-14726] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from UACC-62 cells.Antibodies: Rabbit anti-STAT6 recombinant monoclonalantibody used for IP at 20ul/mg lysate. STAT6 was also immunoprecipitated by otherantibodies against different epitopes of STAT6. For blotting immunoprecipitatedSTAT6, was used at 1:1000. Chemiluminescencewith an exposure time of 30 seconds.")
![Flow Cytometry: CD3 Antibody (G4.18) [NBP1-43458] - Analysis using the Biotin conjugate of NBP1-43458. Staining of rat splenocytes with anti-Rat CD45R (B220) FITC and staining buffer (autofluorescence) (left) or 0.125 ug of anti-Rat CD3 Biotin (right) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/CD3-Antibody-G4-18-Flow-Cytometry-NBP1-43458-img0003.jpg "Flow Cytometry: CD3 Antibody (G4.18) [NBP1-43458] - Analysis using the Biotin conjugate of NBP1-43458. Staining of rat splenocytes with anti-Rat CD45R (B220) FITC and staining buffer (autofluorescence) (left) or 0.125 ug of anti-Rat CD3 Biotin (right) followed by Streptavidin PE.")
![Flow Cytometry: CD3 Antibody (G4.18) [NBP1-43458] - Staining of rat splenocytes with 0.25 ug of Mouse IgG3 Isotype control (open histogram) or 0.25 ug of Anti-Rat CD3 (filled histogram) followed by anti-Mouse IgG Biotin and Streptavidin PE.](http://images.novusbio.com/fullsize/CD3-Antibody-G4-18-Flow-Cytometry-NBP1-43458-img0001.jpg)
![Western Blot: Caspase-1 Antibody [NBP1-76605] - Human heart cell lysate (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Western-Blot-NBP1-76605-img0003.jpg "Western Blot: Caspase-1 Antibody [NBP1-76605] - Human heart cell lysate (A) 1 and (B) 2 ug/ml.")
![Immunohistochemistry-Paraffin: Caspase-1 Antibody [NBP1-76605] - Human heart tissue using Caspase-1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-76605-img0002.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NB100-41104] - Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NB100-41104-img0003.jpg "Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NB100-41104] - Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts.")
![Western Blot: FLAP Antibody [NB100-53797] - Human U937 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/FLAP-Antibody-Western-Blot-NB100-53797-img0005.jpg "Western Blot: FLAP Antibody [NB100-53797] - Human U937 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: FLAP Antibody [NB100-53797] - (3.8ug/ml) staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/FLAP-Antibody-Immunohistochemistry-Paraffin-NB100-53797-img0004.jpg)
![Western Blot: ASC/TMS1 Antibody [NB100-870] - Staining of HeLa lysate (35 ug protein in RIPA buffer). Antibody at 0.2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NB100-870-img0001.jpg "Western Blot: ASC/TMS1 Antibody [NB100-870] - Staining of HeLa lysate (35 ug protein in RIPA buffer). Antibody at 0.2 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NB100-870] - Staining of paraffin embedded Human Liver. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NB100-870-img0002.jpg)
![Immunohistochemistry-Paraffin: CD3 Antibody (RM0027-3B19) [NBP2-12159] - Mouse kidney section.](http://images.novusbio.com/fullsize/CD3-Antibody-RM0027-3B19-Immunohistochemistry-Paraffin-NBP2-12159-img0002.jpg "Immunohistochemistry-Paraffin: CD3 Antibody (RM0027-3B19) [NBP2-12159] - Mouse kidney section.")
![Immunohistochemistry-Paraffin: CD3 Antibody (RM0027-3B19) [NBP2-12159] - Mouse spleen section.](http://images.novusbio.com/fullsize/CD3-Antibody-RM0027-3B19-Immunohistochemistry-Paraffin-NBP2-12159-img0001.jpg)
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)