| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-130SS | |||
| Azide and BSA Free | NBP2-80761 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77297-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31370SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44662 | |||
| Azide and BSA Free | NBP2-34737-0.1mg | |||
![Western Blot: HIF-1 alpha Antibody (H1alpha 67-7) [NB100-130] - HIF-1 alpha in 1: Cos7 CoCl2 treated cells. 2: Untreated cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-H1alpha-67-7-Western-Blot-NB100-130-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha 67-7) [NB100-130] - HIF-1 alpha in 1: Cos7 CoCl2 treated cells. 2: Untreated cells.")
![Western Blot: STAT6 Antibody [NB600-229] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (15 and 50 ug) cells. Antibody used at 0.1 ug/ml. Bands were detected near predicted molecular weight of 94 kDa for HeLa and NIH3T3 cells.](http://images.novusbio.com/fullsize/STAT6-Antibody-Western-Blot-NB600-229-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: STAT6 Antibody [NB600-229] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (15 and 50 ug) cells. Antibody used at 0.1 ug/ml. Bands were detected near predicted molecular weight of 94 kDa for HeLa and NIH3T3 cells.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: STAT6 Antibody [NB600-229] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- STAT6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunohistochemistry-Paraffin-NB600-229-img0006.jpg)
![Immunoprecipitation: STAT6 Antibody [NB600-229] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells used for IP at 6 ug/mg lysate. STAT6 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated STAT6, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunoprecipitation-NB600-229-img0004.jpg)
![Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Western Blot Validation in Human THP-1 Cells. Loading: 15 ug of lysate per lane.Antibodies: ASC/TMS1 , (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77297_rabbit-polyclonal-asc-tms1-antibody-2711202419553086.gif "Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Western Blot Validation in Human THP-1 Cells. Loading: 15 ug of lysate per lane.Antibodies: ASC/TMS1 , (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Western Blot: ASC/TMS1 Antibody [NBP1-77297] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: ASC/TMS1 (NBP1-77297), (2 ug/mL), ASC, (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit or goat anti-mouse IgG HRP conjugate.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NBP1-77297-img0009.jpg)
![Immunohistochemistry: ASC/TMS1 Antibody [NBP1-77297] - Staining of human spleen tissue with antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-NBP1-77297-img0006.jpg)
![Western Blot: ASC/TMS1 Antibody [NBP1-77297] - Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide with ASC antibody at 1 ug /ml.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NBP1-77297-img0005.jpg)
![Immunohistochemistry: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Validation of ASC/TMS1 in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling ASC/TMS1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77297_rabbit-polyclonal-asc-tms1-antibody-2711202419261825.gif)
![Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Validation of ASC/TMS1 in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-ASC/TMS1 antibody at 5 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77297_rabbit-polyclonal-asc-tms1-antibody-271120241954153.gif)
![Immunohistochemistry-Paraffin: HLA G Antibody (4H84) [NB110-55297] - Staining of human spleen (paraffin sections) using anti-HLA-G (4H84).](http://images.novusbio.com/fullsize/HLA-G-Antibody-4H84-Immunohistochemistry-Paraffin-NB110-55297-img0003.jpg "Immunohistochemistry-Paraffin: HLA G Antibody (4H84) [NB110-55297] - Staining of human spleen (paraffin sections) using anti-HLA-G (4H84).")
![Western Blot: HLA G Antibody (4H84) [NB110-55297] - Analysis of HLA-G in HLA-G1 transfectants (LCL-HLA-G1) by 4H84 antibody-biotin.](http://images.novusbio.com/fullsize/HLA-G-Antibody-4H84-Western-Blot-NB110-55297-img0002.jpg)
![Western Blot: HLA G Antibody (4H84) [NB110-55297] - HLA G Antibody (4h84) [NB110-55297] - Immunoprecipitation of HLA-G from HLA-G1 transfectanted lysates (LCL-HLA-G1) by MEM-G/9 antibody and protein G. HLA-G was detected by 4H84 antibody (NB110-55297) and goat anti-mouse-HRP conjugate in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).](http://images.novusbio.com/fullsize/HLA-G-Antibody-4h84-Immunoprecipitation-NB110-55297-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP1-06014] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using AIF-1/Iba1 Antibody (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06014-img0009.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP1-06014] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using AIF-1/Iba1 Antibody (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP1-06014] - Staining of paraffin embedded Human Cerebellum AIF-1/Iba1 Antibody at 4 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP1-06014-img0007.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP1-06014] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) with AIF-1:Iba1 Antibody, followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP1-06014-img0008.jpg)
![Western Blot: MUC2 Antibody (4L5Y8) [NBP3-16481] - Western blot analysis of extracts of various cell lines, using MUC2 Rabbit mAb (NBP3-16481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MUC2-Antibody-4L5Y8-Western-Blot-NBP3-16481-img0004.jpg "Western Blot: MUC2 Antibody (4L5Y8) [NBP3-16481] - Western blot analysis of extracts of various cell lines, using MUC2 Rabbit mAb (NBP3-16481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: MUC2 Antibody (4L5Y8) [NBP3-16481] - Western blot analysis of extracts of Rat large intestine, using MUC2 Rabbit mAb (NBP3-16481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/MUC2-Antibody-4L5Y8-Western-Blot-NBP3-16481-img0005.jpg)
![Immunohistochemistry: MUC2 Antibody (4L5Y8) [NBP3-16481] - Immunohistochemistry analysis of paraffin-embedded Human colon using MUC2 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16481_rabbit-muc2-mab-4l5y8-52202511383779.jpg)
![Immunohistochemistry: MUC2 Antibody (4L5Y8) [NBP3-16481] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MUC2 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16481_rabbit-muc2-mab-4l5y8-5220251136510.jpg)
![Immunohistochemistry: NUT Antibody (PSH02-78) [NBP3-32673] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUT antibody (NBP3-32673) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32673) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32673_rabbit-nut-mab-psh02-78-298202413585581.jpg "Immunohistochemistry: NUT Antibody (PSH02-78) [NBP3-32673] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUT antibody (NBP3-32673) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32673) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: NUT Antibody (PSH02-78) [NBP3-32673] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling NUT with Rabbit anti-NUT antibody (NBP3-32673) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32673, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32673_rabbit-nut-mab-psh02-78-298202413585547.jpg)
![Immunocytochemistry/ Immunofluorescence: NUT Antibody (PSH02-78) [NBP3-32673] - Immunocytochemistry analysis of 293T transfacted with or without mouse NUT cells labeling Nutm1 with Rabbit anti-NUT antibody (NBP3-32673) at 1/500 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NUT antibody (NBP3-32673) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32673_rabbit-nut-mab-psh02-78-2920241192316.jpg)
![Western blot analysis on different lysates with NBP3-32673 at 1/2,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32673 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32673_-western-blot-639204597142008496.jpg)
![Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Separation of HLA-G transfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-BSA-Free-Flow-Cytometry-NBP1-44926-img0004.jpg "Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Separation of HLA-G transfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.")
![Flow Cytometry: HLA G Antibody (01G) [NBP1-44926] - Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) purified, GAM-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-Flow-Cytometry-NBP1-44926-img0001.jpg)
![Flow Cytometry: HLA G Antibody (01G) [NBP1-44926] - Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-Flow-Cytometry-NBP1-44926-img0002.jpg)
![Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Surface staining pattern of HLA-G transfected LCL cells using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-BSA-Free-Flow-Cytometry-NBP1-44926-img0003.jpg)
![Western Blot: HLA G Antibody (5A6G7) [NBP1-44924] - Analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector Lane 2: M8 cell line transfected with HLA-G1 Lane 3: M8 cell line transfected with HLA-G5 Lane 4: M8 cell line transfected with HLA-G6](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Western-Blot-NBP1-44924-img0001.jpg "Western Blot: HLA G Antibody (5A6G7) [NBP1-44924] - Analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector Lane 2: M8 cell line transfected with HLA-G1 Lane 3: M8 cell line transfected with HLA-G5 Lane 4: M8 cell line transfected with HLA-G6")
![Flow Cytometry: HLA G Antibody (5A6G7) [NBP1-44924] - Staining of HLA-G in HLA-G1 transfectants with anti-HLA-G (5A6G7) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Flow-Cytometry-NBP1-44924-img0003.jpg)
![Flow Cytometry: HLA G Antibody (5A6G7) [NBP1-44924] - Staining of HLA-G in HLA-G6 transfectants with anti-HLA-G (5A6G7) PE.](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Flow-Cytometry-NBP1-44924-img0002.jpg)
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA.<br/><br/> Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA.<br/><br/> Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution.<br/><br/> Lane 1: THP-1 cell lysate<br/> Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 31 kDa<br/> Observed band size: 31 kDa<br/><br/> Exposure time: 1 minute;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)
![Western blot analysis on different lysates with NBP3-32464 at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 35/17 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32464 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_-western-blot-639204590073356618.jpg)
![Western blot analysis of different proteins with NBP3-32464 at 1/2,000 dilution. Lane 1: Recombinant mouse IL-1 beta Lane 2: Recombinant rat IL-1 beta Lane 3: Recombinant human IL-1 beta Lysates/proteins at 50 ng/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32464 at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_-western-blot-639204597792672447.jpg)
![Western Blot: NUT Antibody [NBP2-31370] - WB detection of NUT/ NUTM1 protein in (A) human testis lysate and on (B) partial recombinant NUT protein with NUT antibody at a concentration of 2 ug/ml. In human testis, this antibody detected Isoform 1 and Isoform 2 of NUT protein at ~120 kDa and ~46kDa respectively.](http://images.novusbio.com/fullsize/NUT-Antibody-Western-Blot-NBP2-31370-img0001.jpg "Western Blot: NUT Antibody [NBP2-31370] - WB detection of NUT/ NUTM1 protein in (A) human testis lysate and on (B) partial recombinant NUT protein with NUT antibody at a concentration of 2 ug/ml. In human testis, this antibody detected Isoform 1 and Isoform 2 of NUT protein at ~120 kDa and ~46kDa respectively.")
![Immunocytochemistry/Immunofluorescence: NUT Antibody [NBP2-31370] - NUT antibody was tested at 0.01 ug/ml in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/NUT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31370-img0005.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SPM132) [NBP2-32877] - Formalin-fixed, paraffin-embedded human Breast cancer stained with EMA Monoclonal Antibody (SPM132)](http://images.novusbio.com/fullsize/MUC1-Antibody-SPM132-Immunohistochemistry-Paraffin-NBP2-32877-img0001.jpg)
