| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44489 | |||
| BSA Free | NBP2-14783-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-130SS | |||
| Azide and BSA Free | NBP2-80761 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-726SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27342SS | |||
| Azide and BSA Free | NBP2-80791 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05122-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62565-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62561-0.025mg | |||
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Analysis in human tonsil and skeletal muscle tissues using NBP2-14783 antibody. Corresponding PTPRC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411351..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Analysis in human tonsil and skeletal muscle tissues using NBP2-14783 antibody. Corresponding PTPRC RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD45 Antibody (CL0160) [NBP2-14783] - Western Blot analysis using CD45 antibody (CL0160) [NBP2-14783]. Lane 1: Marker [kDa]. Lane 2: Human cell line Jurkat. Lane 3: Human cell line MCF-7. Observed molecular weight ~230 kDa.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0160-Western-Blot-NBP2-14783-img0007.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] - Staining of human tonsil using CD45 antibody (CL0160) [NBP2-14783] shows strong positivity in a majority of lymphoid cells](http://images.novusbio.com/fullsize/CD45-Antibody-CL0160-Immunohistochemistry-Paraffin-NBP2-14783-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411445..jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Staining of human duodenum shows moderate membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411549..jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411643..jpg)
![Western Blot: HIF-1 alpha Antibody (H1alpha 67-7) [NB100-130] - HIF-1 alpha in 1: Cos7 CoCl2 treated cells. 2: Untreated cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-H1alpha-67-7-Western-Blot-NB100-130-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha 67-7) [NB100-130] - HIF-1 alpha in 1: Cos7 CoCl2 treated cells. 2: Untreated cells.")
![Western Blot: LYVE-1 Antibody [NB100-726] - Detection of LYVE-1 in recombinant human protein using NB 100-726. ECL detection overnight.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Western-Blot-NB100-726-img0006.jpg "Western Blot: LYVE-1 Antibody [NB100-726] - Detection of LYVE-1 in recombinant human protein using NB 100-726. ECL detection overnight.")
![Western Blot: LYVE-1 Antibody [NB100-726] - Detection of LYVE-1 in mouse spleen lysate using NB 100-726. ECL detection overnight.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Western-Blot-NB100-726-img0005.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N1G6) [NBP2-27342] - Analysis of IL-1beta in A) full-length recombinant protein and B) human spleen lysate using IL-1beta antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N1G6-Western-Blot-NBP2-27342-img0002.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (43N1G6) [NBP2-27342] - Analysis of IL-1beta in A) full-length recombinant protein and B) human spleen lysate using IL-1beta antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP1-06014] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using AIF-1/Iba1 Antibody (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06014-img0009.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP1-06014] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using AIF-1/Iba1 Antibody (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP1-06014] - Staining of paraffin embedded Human Cerebellum AIF-1/Iba1 Antibody at 4 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP1-06014-img0007.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP1-06014] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) with AIF-1:Iba1 Antibody, followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP1-06014-img0008.jpg)
![Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05122-img0011.jpg "Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.")
![Western Blot: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Lane 1: purified C3, lane 2: purifed C3 reduced/DTT, lane 3: purified C3a , lane 4: EDTA-plasma. Lane 5: zymosan-activated serum](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Western-Blot-NBP1-05122-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining of human kidney. Strong staining of veins of nephritis kidney was seen. Dilution: 1:150.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05122-img0007.jpg)
![Sandwich ELISA Capture: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - The calibration curve of a sandwich assay for Complement C3a using NBP1-05122 as the capture antibody and NBP2-23496 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Sandwich-ELISA-Capture-NBP1-05122-img0005.jpg)
![Western Blot: HLA G Antibody (5A6G7) [NBP1-44924] - Analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector Lane 2: M8 cell line transfected with HLA-G1 Lane 3: M8 cell line transfected with HLA-G5 Lane 4: M8 cell line transfected with HLA-G6](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Western-Blot-NBP1-44924-img0001.jpg "Western Blot: HLA G Antibody (5A6G7) [NBP1-44924] - Analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector Lane 2: M8 cell line transfected with HLA-G1 Lane 3: M8 cell line transfected with HLA-G5 Lane 4: M8 cell line transfected with HLA-G6")
![Flow Cytometry: HLA G Antibody (5A6G7) [NBP1-44924] - Staining of HLA-G in HLA-G1 transfectants with anti-HLA-G (5A6G7) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Flow-Cytometry-NBP1-44924-img0003.jpg)
![Flow Cytometry: HLA G Antibody (5A6G7) [NBP1-44924] - Staining of HLA-G in HLA-G6 transfectants with anti-HLA-G (5A6G7) PE.](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Flow-Cytometry-NBP1-44924-img0002.jpg)
![Western Blot: NUT Antibody [NBP3-32672] - Western blot analysis of NUT on mouse testis tissue lysates with Rabbit anti-NUT antibody (NBP3-32672) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32672) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32672_rabbit-nut-mab-278202416331244.jpg "Western Blot: NUT Antibody [NBP3-32672] - Western blot analysis of NUT on mouse testis tissue lysates with Rabbit anti-NUT antibody (NBP3-32672) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32672) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NUT Antibody [NBP3-32672] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUT antibody (NBP3-32672) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32672) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32672_rabbit-nut-mab-29820241411542.jpg)
![Immunohistochemistry: NUT Antibody [NBP3-32672] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling NUT with Rabbit anti-NUT antibody (NBP3-32672) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32672, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32672_rabbit-nut-mab-298202414115413.jpg)
![Immunocytochemistry/ Immunofluorescence: NUT Antibody [NBP3-32672] - Immunocytochemistry analysis of 293T transfacted with or without mouse NUT cells labeling Nutm1 with Rabbit anti-NUT antibody (NBP3-32672) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NUT antibody (NBP3-32672) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32672_rabbit-nut-mab-29202410484895.jpg)
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunocytochemistry-Immunofluorescence-NBP2-67865-img0003.jpg "Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Analysis of paraffin-embedded human kidney tissue using anti-IL1 beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunohistochemistry-Paraffin-NBP2-67865-img0004.jpg)
![Flow Cytometry: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Analysis of Hela cells with IL1 beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Flow-Cytometry-NBP2-67865-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunocytochemistry-Immunofluorescence-NBP2-67865-img0002.jpg)
![Western Blot: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Western Blot using anti-MUC1 antibody HMFG2. MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of HMFG2 (NBP2-62565) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa, though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. NBP2-62565 successfully detected human MUC1 in MCF-7 breast cancer cells.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Western-Blot-NBP2-62565-img0001.jpg "Western Blot: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Western Blot using anti-MUC1 antibody HMFG2. MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of HMFG2 (NBP2-62565) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa, though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. NBP2-62565 successfully detected human MUC1 in MCF-7 breast cancer cells.")
![Immunohistochemistry-Paraffin: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Immunohistochemical staining of human lung tissue using anti-MUC1 antibody. HMFG2 Anti-MUC1 (Mucin-1) staining of paraffin embedded human lung tissue using the rabbit-chimeric version of HMFG2 (NBP2-62565). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62565 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong staining of type II pneumocytes may be observed. Recommended concentration, 1-2 ug/ml.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Immunohistochemistry-Paraffin-NBP2-62565-img0003.jpg)
![Flow Cytometry: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Flow-cytometry using the anti-MUC1 antibody HMFG2. MCF-7 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of HMFG2 (NBP2-62565, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Flow-Cytometry-NBP2-62565-img0002.jpg)
![Immunofluorescence: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Immunofluorescence staining of fixed MCF-7 cells with anti-MUC1 antibody HMFG2. Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed MCF-7 cells on Shi-fix coverslips stained with the chimeric rabbit version of HMFG2 (NBP2-62565) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62565, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Immunofluorescence-NBP2-62565-img0004.jpg)
![Western Blot: MUC1 Antibody (SM3) - Chimeric [NBP2-62561] - Western Blot using anti-MUC1 antibody SM3. MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of SM3 (NBP2-62561) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa, though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. NBP2-62561 successfully detected human MUC1 in MCF-7 breast cancer cells.](http://images.novusbio.com/fullsize/MUC1-Antibody-SM3-Chimeric-Western-Blot-NBP2-62561-img0002.jpg "Western Blot: MUC1 Antibody (SM3) - Chimeric [NBP2-62561] - Western Blot using anti-MUC1 antibody SM3. MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of SM3 (NBP2-62561) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa, though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. NBP2-62561 successfully detected human MUC1 in MCF-7 breast cancer cells.")
![Immunohistochemistry: MUC1 Antibody (SM3) - Chimeric [NBP2-62561] - Immunohistochemical staining of human lung tissue using anti-MUC1 antibody. SM3 Anti-MUC1 (Mucin-1) staining of paraffin embedded human lung tissue using the rabbit-chimeric version of SM3 (NBP2-62561). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62561 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of type II pneumocytes may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/MUC1-Antibody-SM3-Chimeric-Immunohistochemistry-NBP2-62561-img0001.jpg)
![Flow Cytometry: MUC1 Antibody (SM3) - Chimeric [NBP2-62561] - Flow-cytometry using the anti-MUC1 SM3. MCF-7 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of SM3 (NBP2-62561, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/MUC1-Antibody-SM3-Chimeric-Flow-Cytometry-NBP2-62561-img0003.jpg)
![Immunofluorescence: MUC1 Antibody (SM3) - Chimeric [NBP2-62561] - Immunofluorescence staining of fixed MCF-7 cells with anti-MUC1 antibody SM3. Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed MCF-7 cells on Shi-fix coverslips stained with the chimeric rabbit version of SM3 (NBP2-62561) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing clear membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom; NBP2-62561, DAPI, merged channels and an isotype control. The isotype control was stained with unimmunised rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/MUC1-Antibody-SM3-Chimeric-Immunofluorescence-NBP2-62561-img0004.jpg)
![Immunoprecipitation: STAT6 Antibody [NBP2-20506] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of STAT6 protein by 2 ug of STAT6 antibody 10% SDS-PAGE The immunoprecipitated STAT6 protein was detected by STAT6 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunoprecipitation-NBP2-20506-img0004.jpg "Immunoprecipitation: STAT6 Antibody [NBP2-20506] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of STAT6 protein by 2 ug of STAT6 antibody 10% SDS-PAGE The immunoprecipitated STAT6 protein was detected by STAT6 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.")
