| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47886-0.1mg | |||
| IHC-Prediluted | NBP2-48375 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44853 | |||
| Azide and BSA Free | NBP2-34527-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14276-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84666-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77297-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97471-0.2mg | |||
| BSA Free | NBP1-97471-0.025mg | |||
![Western Blot: MUC1 Antibody (MUC1/520) [NBP2-44660] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Western-Blot-NBP2-44660-img0007.jpg "Western Blot: MUC1 Antibody (MUC1/520) [NBP2-44660] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/520).")
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/520) [NBP2-44660] - Human Ovarian Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Immunohistochemistry-Paraffin-NBP2-44660-img0006.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/520) [NBP2-44660] - Human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Immunohistochemistry-Paraffin-NBP2-44660-img0001.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/520) [NBP2-44660] - Human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Immunohistochemistry-Paraffin-NBP2-44660-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/520) [NBP2-44660] - Human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Immunohistochemistry-Paraffin-NBP2-44660-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/520) [NBP2-44660] - Human Colon Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Immunohistochemistry-Paraffin-NBP2-44660-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/520) [NBP2-44660] - Human Endometrial Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-520-Immunohistochemistry-Paraffin-NBP2-44660-img0005.jpg)
![Formalin-fixed, paraffin-embedded human tonsil stained with CD45 antibody (2B11) [NBP2-32934].](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-32934-img0002.jpg "Formalin-fixed, paraffin-embedded human tonsil stained with CD45 antibody (2B11) [NBP2-32934].")
![Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD45 antibody (2B11) [NBP2-32934].](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-32934-img0001.jpg)
![Western Blot: STAT6 Antibody (6C10) [H00006778-M01] - Analysis of STAT6 monoclonal antibody (M01, clone 6C10) expression in HeLa. Band was detected at a molecular weight of approximately 94 kDa.](http://images.novusbio.com/fullsize/STAT6-Antibody-6C10-Western-Blot-H00006778-M01-img0010.jpg "Western Blot: STAT6 Antibody (6C10) [H00006778-M01] - Analysis of STAT6 monoclonal antibody (M01, clone 6C10) expression in HeLa. Band was detected at a molecular weight of approximately 94 kDa.")
![Immunocytochemistry/Immunofluorescence: STAT6 Antibody (6C10) [H00006778-M01] - Analysis of monoclonal antibody to STAT6 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/STAT6-Antibody-6C10-Immunocytochemistry-Immunofluorescence-H00006778-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: STAT6 Antibody (6C10) [H00006778-M01] - Analysis of monoclonal antibody to STAT6 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/STAT6-Antibody-6C10-Immunohistochemistry-Paraffin-H00006778-M01-img0008.jpg)
![Western Blot: STAT6 Antibody (6C10) [H00006778-M01] - Analysis of STAT6 expression in transfected 293T cell line by STAT6 monoclonal antibody (M01, clone 6C10). Lane 1: STAT6 transfected lysate (Molecular Weight of approximately 94 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/STAT6-Antibody-6C10-Western-Blot-H00006778-M01-img0011.jpg)
![Sandwich ELISA: STAT6 Antibody (6C10) [H00006778-M01] - Detection limit for recombinant GST tagged STAT6 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/STAT6-Antibody-6C10-Sandwich-ELISA-H00006778-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Analysis in human spleen and skeletal muscle tissues using . Corresponding AIF-1/Iba1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0001.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Analysis in human spleen and skeletal muscle tissues using . Corresponding AIF-1/Iba1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human spleen using AIF-1/Iba1 Antibody shows very strong membranous positivity in cells in red pulp.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human cerebral cortex using AIF-1/Iba1 Antibody shows strong membranous positivity in glial cells.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human kidney using AIF-1/Iba1 Antibody shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human lymphoid tissues using AIF-1/Iba1 Antibody shows very strong membranous positivity in non-germinal, and germinal center cells.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human placenta using AIF-1/Iba1 Antibody shows very strong cytoplasmic positivity in Hofbauer cells.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human skeletal muscle using AIF-1/Iba1 Antibody shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0006.jpg)
![Immunohistochemistry-Paraffin: FLAP Antibody [NBP1-84666] - Staining in human bone marrow and skeletal muscle tissues using anti-ALOX5AP antibody. Corresponding ALOX5AP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FLAP-Antibody-Immunohistochemistry-Paraffin-NBP1-84666-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FLAP Antibody [NBP1-84666] - Staining in human bone marrow and skeletal muscle tissues using anti-ALOX5AP antibody. Corresponding ALOX5AP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FLAP Antibody [NBP1-84666] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/FLAP-Antibody-Immunohistochemistry-Paraffin-NBP1-84666-img0006.jpg)
![Immunohistochemistry-Paraffin: FLAP Antibody [NBP1-84666] - Staining of human bone marrow shows cytoplasmic and nuclear membrane positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/FLAP-Antibody-Immunohistochemistry-Paraffin-NBP1-84666-img0005.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/nbp1-84666_rabbit-polyclonal-flap-antibody-104202515371765.jpg)
![Western Blot: STAT6 Antibody [NB600-229] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (15 and 50 ug) cells. Antibody used at 0.1 ug/ml. Bands were detected near predicted molecular weight of 94 kDa for HeLa and NIH3T3 cells.](http://images.novusbio.com/fullsize/STAT6-Antibody-Western-Blot-NB600-229-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: STAT6 Antibody [NB600-229] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (15 and 50 ug) cells. Antibody used at 0.1 ug/ml. Bands were detected near predicted molecular weight of 94 kDa for HeLa and NIH3T3 cells.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: STAT6 Antibody [NB600-229] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- STAT6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunohistochemistry-Paraffin-NB600-229-img0006.jpg)
![Immunoprecipitation: STAT6 Antibody [NB600-229] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells used for IP at 6 ug/mg lysate. STAT6 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated STAT6, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunoprecipitation-NB600-229-img0004.jpg)
![Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Western Blot Validation in Human THP-1 Cells. Loading: 15 ug of lysate per lane.Antibodies: ASC/TMS1 , (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77297_rabbit-polyclonal-asc-tms1-antibody-2711202419553086.gif "Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Western Blot Validation in Human THP-1 Cells. Loading: 15 ug of lysate per lane.Antibodies: ASC/TMS1 , (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Western Blot: ASC/TMS1 Antibody [NBP1-77297] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: ASC/TMS1 (NBP1-77297), (2 ug/mL), ASC, (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit or goat anti-mouse IgG HRP conjugate.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NBP1-77297-img0009.jpg)
![Immunohistochemistry: ASC/TMS1 Antibody [NBP1-77297] - Staining of human spleen tissue with antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-NBP1-77297-img0006.jpg)
![Western Blot: ASC/TMS1 Antibody [NBP1-77297] - Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide with ASC antibody at 1 ug /ml.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NBP1-77297-img0005.jpg)
![Immunohistochemistry: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Validation of ASC/TMS1 in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling ASC/TMS1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77297_rabbit-polyclonal-asc-tms1-antibody-2711202419261825.gif)
![Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Validation of ASC/TMS1 in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-ASC/TMS1 antibody at 5 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77297_rabbit-polyclonal-asc-tms1-antibody-271120241954153.gif)
![Immunohistochemistry-Paraffin: HLA G Antibody (4H84) [NB110-55297] - Staining of human spleen (paraffin sections) using anti-HLA-G (4H84).](http://images.novusbio.com/fullsize/HLA-G-Antibody-4H84-Immunohistochemistry-Paraffin-NB110-55297-img0003.jpg "Immunohistochemistry-Paraffin: HLA G Antibody (4H84) [NB110-55297] - Staining of human spleen (paraffin sections) using anti-HLA-G (4H84).")
![Western Blot: HLA G Antibody (4H84) [NB110-55297] - Analysis of HLA-G in HLA-G1 transfectants (LCL-HLA-G1) by 4H84 antibody-biotin.](http://images.novusbio.com/fullsize/HLA-G-Antibody-4H84-Western-Blot-NB110-55297-img0002.jpg)
![Western Blot: HLA G Antibody (4H84) [NB110-55297] - HLA G Antibody (4h84) [NB110-55297] - Immunoprecipitation of HLA-G from HLA-G1 transfectanted lysates (LCL-HLA-G1) by MEM-G/9 antibody and protein G. HLA-G was detected by 4H84 antibody (NB110-55297) and goat anti-mouse-HRP conjugate in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).](http://images.novusbio.com/fullsize/HLA-G-Antibody-4h84-Immunoprecipitation-NB110-55297-img0001.jpg)
![Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.](http://images.novusbio.com/fullsize/iNOS-Antibody-Western-Blot-NBP1-97471-img0001.jpg "Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.")
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-immunocytochemistry-immunofluorescence-2972024113947..jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/1,000 dilution.Lane 1: Raji cell lysate (10 ug/Lane)Lane 2: Mouse lung tissue lysate (20 ug/Lane)Lane 3: Rat lung tissue lysate (20 ug/Lane)Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114129..jpg)
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/2,000 dilution.Lane 1: Daudi cell lysateLane 2: Raji cell lysateLane 3: Ramos cell lysateLane 4: Jurkat cell lysateLane 5: K-562 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 2 minutes 37 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114258..jpg)
![Western Blot: NUT Antibody (PSH02-78) [NBP3-32673] - Western blot analysis of NUT on different lysates with Rabbit anti-NUT antibody (NBP3-32673) at 1/2,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32673) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32673_rabbit-nut-mab-psh02-78-278202415242684.jpg "Western Blot: NUT Antibody (PSH02-78) [NBP3-32673] - Western blot analysis of NUT on different lysates with Rabbit anti-NUT antibody (NBP3-32673) at 1/2,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32673) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NUT Antibody (PSH02-78) [NBP3-32673] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUT antibody (NBP3-32673) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32673) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32673_rabbit-nut-mab-psh02-78-298202413585581.jpg)
![Immunohistochemistry: NUT Antibody (PSH02-78) [NBP3-32673] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling NUT with Rabbit anti-NUT antibody (NBP3-32673) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32673, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32673_rabbit-nut-mab-psh02-78-298202413585547.jpg)
![Immunocytochemistry/ Immunofluorescence: NUT Antibody (PSH02-78) [NBP3-32673] - Immunocytochemistry analysis of 293T transfacted with or without mouse NUT cells labeling Nutm1 with Rabbit anti-NUT antibody (NBP3-32673) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NUT antibody (NBP3-32673) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32673_rabbit-nut-mab-psh02-78-2920241192316.jpg)
![Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Separation of HLA-G transfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-BSA-Free-Flow-Cytometry-NBP1-44926-img0004.jpg "Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Separation of HLA-G transfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.")
![Flow Cytometry: HLA G Antibody (01G) [NBP1-44926] - Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) purified, GAM-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-Flow-Cytometry-NBP1-44926-img0001.jpg)
![Flow Cytometry: HLA G Antibody (01G) [NBP1-44926] - Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-Flow-Cytometry-NBP1-44926-img0002.jpg)
![Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Surface staining pattern of HLA-G transfected LCL cells using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-BSA-Free-Flow-Cytometry-NBP1-44926-img0003.jpg)