| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81805-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86862-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81806-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94087-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57150-25ul | |||
![Simple Western: VPS34 Antibody [NB110-87320] - Simple Western lane view shows a specific band for VPS34 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/VPS34-Antibody-Simple-Western-NB110-87320-img0006.jpg "Simple Western: VPS34 Antibody [NB110-87320] - Simple Western lane view shows a specific band for VPS34 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Genetic Strategies Validation. </b>Western Blot: VPS34 Antibody [NB110-87320] - ATG7 depleted SKBR3 cells show increased sensitivity to ATRA and the RARalpha agonist AM580. RARalpha , ATG5 and VPS34 Western blot analysis of control and the respective SKBR3 knockdown cells. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VPS34-Antibody-Knockdown-Validated-NB110-87320-img0007.jpg)
![Western Blot: VPS34 Antibody [NB110-87320] - HepG2 whole cell lysates.](http://images.novusbio.com/fullsize/VPS34-Antibody-Western-Blot-NB110-87320-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: VPS34 Antibody [NB110-87320] - VPS34 antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/VPS34-Antibody-Immunocytochemistry-Immunofluorescence-NB110-87320-img0005.jpg)
![Western Blot: VPS34 Antibody [NB110-87320] - Celastrol regulates autophagy- & mitophagy-related gene expressions in neurons. SH-SY5Y cells treated with 0.1–1 μM celastrol for 4 h. (A) Real-time quantitative PCR results show that 1 μM celastrol treatment for 4 h enhanced mRNA expressions of phagophore induction genes Beclin 1 & Ambra1, phagophore elongation genes Atg7 & Atg12, & vacuole formation genes LC3A & Atg4B. (B) Western blot results of SH-SY5Y treated with 100–500 nM celastrol show celastrol (500 nM) increased in protein expressions of Beclin 1 & Vps34 (phagophore induction), Atg7 (phagophore elongation), LC3-II (vacuole formation), DJ-1, & PINK1 (mitophagy). Celastrol (500 nM) suppressed LRRK2 expression. (C) MPTP (10 mg/kg/day for 3 days, i.p.) but not celastrol (3 mg/kg/day for 3 days, i.p.) caused dopaminergic nerve terminal degeneration (tyrosine hydroxylase↓) & mitophagy inactivation (DJ-1↓ & PINK1↓) in the striatum of mice as compared to saline control. Celastrol cotreatment with MPTP reversed it as compared to MPTP. Data are given as the mean ± SEM (n = 3–5). p-value was determined using the Kruskal–Wallis test followed by Dunn’s multiple comparison post hoc test. * p < 0.05 compared with controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906147), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-87320_rabbit-polyclonal-vps34-antibody-310202416205164.jpg)
![Immunocytochemistry/Immunofluorescence: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - VPS34 antibody [HL2305] detects VPS34 protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS34 stained by VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-2120249413371.jpg "Immunocytochemistry/Immunofluorescence: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - VPS34 antibody [HL2305] detects VPS34 protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS34 stained by VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-2120249425434.jpg)
![Western Blot: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-2120249415921.jpg)
![Western Blot: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-2120249425433.jpg)
![Immunohistochemistry-Paraffin: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - VPS34 antibody [HL2305] detects VPS34 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VPS34 stained by VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-2120249342719.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: VPS34 Antibody (HL2305) - Azide and BSA Free [NBP3-25745] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-212024939136.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (NBP3-25745) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25745_rabbit-vps34-mab-hl2305-azide-and-bsa-free-western-blot-14112025182608.jpg)
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81805] -Analysis in human testis and pancreas tissues using antibody. Corresponding CLEC16A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-81805_rabbit-polyclonal-clec16a-antibody-immunohistochemistry-paraffin-3820238318..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81805] -Analysis in human testis and pancreas tissues using antibody. Corresponding CLEC16A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81805] -Staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp1-81805_rabbit-polyclonal-clec16a-antibody-immunohistochemistry-paraffin-38202383247..jpg)
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81805] -Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/antibody/nbp1-81805_rabbit-polyclonal-clec16a-antibody-immunohistochemistry-paraffin-38202383534..jpg)
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81805] -Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-81805_rabbit-polyclonal-clec16a-antibody-immunohistochemistry-paraffin-38202384025..jpg)
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81805] -Staining of human pancreas shows very weak positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-81805_rabbit-polyclonal-clec16a-antibody-immunohistochemistry-paraffin-38202384141..jpg)
![Immunohistochemistry-Paraffin: VPS34 Antibody [NBP1-86862] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/VPS34-Antibody-Immunohistochemistry-Paraffin-NBP1-86862-img0008.jpg "Immunohistochemistry-Paraffin: VPS34 Antibody [NBP1-86862] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.")
![Immunohistochemistry-Paraffin: VPS34 Antibody [NBP1-86862] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/VPS34-Antibody-Immunohistochemistry-Paraffin-NBP1-86862-img0007.jpg)
![Western Blot: VPS34 Antibody (9U5O3) [NBP3-15441] - Western blot analysis of extracts of various cell lines, using VPS34 Rabbit mAb (NBP3-15441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/VPS34-Antibody-9U5O3-Western-Blot-NBP3-15441-img0002.jpg "Western Blot: VPS34 Antibody (9U5O3) [NBP3-15441] - Western blot analysis of extracts of various cell lines, using VPS34 Rabbit mAb (NBP3-15441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: VPS34 Antibody (9U5O3) [NBP3-15441] - Immunofluorescence analysis of NIH-3T3 cells using VPS34 Rabbit mAb (NBP3-15441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VPS34-Antibody-9U5O3-Immunocytochemistry-Immunofluorescence-NBP3-15441-img0001.jpg)
![Immunoprecipitation: VPS34 Antibody (9U5O3) [NBP3-15441] - Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug VPS34 antibody (NBP3-15441). Western blot was performed from the immunoprecipitate using VPS34 antibody (NBP3-15441) at a dilition of 1:500.](http://images.novusbio.com/fullsize/VPS34-Antibody-9U5O3-Immunoprecipitation-NBP3-15441-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: VPS34 Antibody (9U5O3) [VPS34] - Confocal imaging of C2C12 cells using VPS34 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15441_rabbit-vps34-mab-9u5o3-52202517235772.jpg)
![Immunocytochemistry/ Immunofluorescence: VPS34 Antibody (9U5O3) [VPS34] - Confocal imaging of PC-3 cells using VPS34 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15441_rabbit-vps34-mab-9u5o3-52202517273563.jpg)
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81806] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/CLEC16A-Antibody-Immunohistochemistry-Paraffin-NBP1-81806-img0010.jpg "Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81806] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.")
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81806] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/CLEC16A-Antibody-Immunohistochemistry-Paraffin-NBP1-81806-img0007.jpg)
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81806] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CLEC16A-Antibody-Immunohistochemistry-Paraffin-NBP1-81806-img0008.jpg)
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81806] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CLEC16A-Antibody-Immunohistochemistry-Paraffin-NBP1-81806-img0009.jpg)
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81806] - Staining of human tonsil shows weak to moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/CLEC16A-Antibody-Immunohistochemistry-Paraffin-NBP1-81806-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CLEC16A Antibody [NBP1-00171] - Analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear and weak cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/CLEC16A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00171-img0001.jpg "Immunocytochemistry/Immunofluorescence: CLEC16A Antibody [NBP1-00171] - Analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear and weak cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: CLEC16A Antibody [NBP1-00171] - Analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CLEC16A-Antibody-Flow-Cytometry-NBP1-00171-img0002.jpg)
![Western Blot: VPS34 Antibody [NBP2-94087] - Western blot analysis of extracts of various cell lines, using VPS34 antibody (NBP2-94087) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/VPS34 Antibody-Western Blot-NBP2-94087-img0002.jpg "Western Blot: VPS34 Antibody [NBP2-94087] - Western blot analysis of extracts of various cell lines, using VPS34 antibody (NBP2-94087) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: VPS34 Antibody [NBP2-94087] - Immunofluorescence analysis of NIH/3T3 cells using VPS34 Rabbit pAb (NBP2-94087) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VPS34-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94087-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CLEC16A Antibody [NBP2-57150] - Staining of human cell line A549 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CLEC16A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57150-img0001.jpg "Immunocytochemistry/Immunofluorescence: CLEC16A Antibody [NBP2-57150] - Staining of human cell line A549 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.")
![Western Blot: CLEC16A Antibody [NBP2-57150] -Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/antibody/nbp2-57150_rabbit-polyclonal-clec16a-antibody-western-blot-209202312112..jpg)
![Immunocytochemistry/Immunofluorescence: CLEC16A Antibody [NBP2-78807] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary antibody incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear and weak cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/CLEC16A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-78807-img0001.jpg "Immunocytochemistry/Immunofluorescence: CLEC16A Antibody [NBP2-78807] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary antibody incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear and weak cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: CLEC16A Antibody [NBP2-78807] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary antibody incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CLEC16A-Antibody-Flow-Cytometry-NBP2-78807-img0002.jpg)