| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37715 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99765-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11984-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08997-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12000-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61611-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52655-0.025mg | |||
![Western Blot: HLA DRB1 Antibody (JE60-43) [NBP3-32433] - Western blot analysis of HLA DRB1 on different lysates with Rabbit anti-HLA DRB1 antibody (NBP3-32433) at 1/1,000 dilution. Lane 1: Ramos cell lysate Lane 2: Raji cell lysate Lane 3: Daudi cell lysate Lane 4: HDLM-2 cell lysate Lane 5: A375 cell lysate Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32433) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32433_rabbit-hla-drb1-mab-je60-43-278202415242697.jpg "Western Blot: HLA DRB1 Antibody (JE60-43) [NBP3-32433] - Western blot analysis of HLA DRB1 on different lysates with Rabbit anti-HLA DRB1 antibody (NBP3-32433) at 1/1,000 dilution. Lane 1: Ramos cell lysate Lane 2: Raji cell lysate Lane 3: Daudi cell lysate Lane 4: HDLM-2 cell lysate Lane 5: A375 cell lysate Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32433) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HLA DRB1 Antibody (JE60-43) [NBP3-32433] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HLA DRB1 antibody (NBP3-32433) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32433) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32433_rabbit-hla-drb1-mab-je60-43-298202414304147.jpg)
![Immunohistochemistry: HLA DRB1 Antibody (JE60-43) [NBP3-32433] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling HLA DRB1 with Rabbit anti-HLA DRB1 antibody (NBP3-32433) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32433, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32433_rabbit-hla-drb1-mab-je60-43-298202414161862.jpg)
![Flow Cytometry: CD2 Antibody (TS1/8) [NBP2-37714] - Staining of CD2 in human peripheral blood with anti-CD2 (TS1/8) PE.](http://images.novusbio.com/fullsize/CD2-Antibody-TS1-8-Flow-Cytometry-NBP2-37714-img0003.jpg "Flow Cytometry: CD2 Antibody (TS1/8) [NBP2-37714] - Staining of CD2 in human peripheral blood with anti-CD2 (TS1/8) PE.")
![Flow Cytometry: CD2 Antibody (TS1/8) [NBP2-37714] - Staining of CD2 in human peripheral blood with anti-CD2 (TS1/8) PE-CyTM7.](http://images.novusbio.com/fullsize/CD2-Antibody-TS1-8-Flow-Cytometry-NBP2-37714-img0001.jpg)
![Flow Cytometry: CD2 Antibody (TS1/8) [NBP2-37714] - Staining of CD2 in human peripheral blood with anti-CD2 (TS1/8) APC-CyTM7.](http://images.novusbio.com/fullsize/CD2-Antibody-TS1-8-Flow-Cytometry-NBP2-37714-img0002.jpg)
![Flow Cytometry: CD2 Antibody (TS1/8) - BSA Free [NBP2-37714] - Surface staining pattern of human peripheral blood stained using anti-human CD2 (TS1/8) purified antibody (low endotoxin, concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/nbp2-37714_mouse-monoclonal-cd2-antibody-ts1-8-17720249362615.jpg)
![Immunohistochemistry-Paraffin: CD5 Antibody (L17F12) [NBP3-09129] - Staining of human tonsil (paraffin-embedded sections) with anti-CD5 (L17F12), 10 ug/ml.](http://images.novusbio.com/fullsize/CD5-Antibody-L17F12-Immunohistochemistry-Paraffin-NBP3-09129-img0001.jpg "Immunohistochemistry-Paraffin: CD5 Antibody (L17F12) [NBP3-09129] - Staining of human tonsil (paraffin-embedded sections) with anti-CD5 (L17F12), 10 ug/ml.")
![Western Blot: CD97 Antibody [NBP2-99765] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Membrane Lysate. Lane B: K562 Membrane Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/CD97-Antibody-Western-Blot-NBP2-99765-img0005.jpg "Western Blot: CD97 Antibody [NBP2-99765] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Membrane Lysate. Lane B: K562 Membrane Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 70 kDa")
![Immunocytochemistry/Immunofluorescence: CD97 Antibody [NBP2-99765] - Immunofluorescence staining of CD97 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human CD97 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/CD97-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99765-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD97 Antibody [NBP2-99765] - Immunofluorescence staining of CD97 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-99765 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/nbp2-99765_rabbit-polyclonal-cd97-antibody-235202511545614.png)
![Flow Cytometry: Siglec-1/CD169 Antibody (7-239) [NBP2-37729] - Staining of CD169 on buffy coat diff. monocytes by anti-CD169 (7-239) PE.](http://images.novusbio.com/fullsize/Siglec-1-CD169-Antibody-7-239-Flow-Cytometry-NBP2-37729-img0001.jpg "Flow Cytometry: Siglec-1/CD169 Antibody (7-239) [NBP2-37729] - Staining of CD169 on buffy coat diff. monocytes by anti-CD169 (7-239) PE.")
![Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32127, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-298202414333631.jpg "Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32127, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32127) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-29820241435244.jpg)
![Immunocytochemistry/ Immunofluorescence: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunocytochemistry analysis of 293T cells labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-2920241192312.jpg)
![Western Blot: ZAP70 Antibody (A1B5-R) [NBP3-33110] - Western blot analysis of ZAP70 on different lysates with Mouse anti-ZAP70 antibody (NBP3-33110) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Ramos cell lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Mouse thymus tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat thymus tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33110_mouse-zap70-mab-a1b5-r-288202414191541.jpg "Western Blot: ZAP70 Antibody (A1B5-R) [NBP3-33110] - Western blot analysis of ZAP70 on different lysates with Mouse anti-ZAP70 antibody (NBP3-33110) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Ramos cell lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Mouse thymus tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat thymus tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ZAP70 Antibody (A1B5-R) [NBP3-33110] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-ZAP70 antibody (NBP3-33110) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33110) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33110_mouse-zap70-mab-a1b5-r-29820241435257.jpg)
![Immunocytochemistry/Immunofluorescence: ChemR23/CMKLR1 Antibody (BZ194) - Chimeric [NBP3-11984] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of BZ194 (NBP3-11984) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11984, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/ChemR23-CMKLR1-Antibody-BZ194-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11984-img0001.jpg "Immunocytochemistry/Immunofluorescence: ChemR23/CMKLR1 Antibody (BZ194) - Chimeric [NBP3-11984] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of BZ194 (NBP3-11984) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11984, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric [NBP3-08997] - Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of AT130-5 (NBP3-08997) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-08997, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RII-CD32-Antibody-AT130-5-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-08997-img0001.jpg "Immunocytochemistry/Immunofluorescence: Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric [NBP3-08997] - Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of AT130-5 (NBP3-08997) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-08997, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![Western Blot: CCR5 Antibody (RC-10) - Chimeric [NBP3-12000] - Jurkat (A), Molt4 (B), K562 (C) and Daudi (D) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RC-10 (NBP3-12000) at 0.0003 ug/ml, 0.001 ug/ml, 0.003 ug/ml and unknown ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CCR5-Antibody-RC-10-Chimeric-Western-Blot-NBP3-12000-img0001.jpg "Western Blot: CCR5 Antibody (RC-10) - Chimeric [NBP3-12000] - Jurkat (A), Molt4 (B), K562 (C) and Daudi (D) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RC-10 (NBP3-12000) at 0.0003 ug/ml, 0.001 ug/ml, 0.003 ug/ml and unknown ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CCR5 Antibody (RC-10) - Chimeric [NBP3-12000] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RC-10 (NBP3-12000) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12000, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CCR5-Antibody-RC-10-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12000-img0002.jpg)
![Flow Cytometry: CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free [NBP3-12000] - Paraformaldehyde fixed human peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12000 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12000_rabbit-monoclonal-ccr5-antibody-rc-10-chimeric-7420252214129.png)
![Immunohistochemistry: TNF alpha Variant Antibody (Z74P2C5*A12) [NBP2-61611] - Immunohistochemistry image showing positive staining of TNF alpha Variant Antibody (Z74P2C5*A12) in adipose tissue vaccinated rainbow trout. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required, which involves microwaving tissue for 20 minutes @ 800W in 10 mM citrate buffer (pH 6.0).](http://images.novusbio.com/fullsize/TNF-alpha-Variant-Antibody-Z74P2C5-A12-Immunohistochemistry-NBP2-61611-img0001.jpg "Immunohistochemistry: TNF alpha Variant Antibody (Z74P2C5*A12) [NBP2-61611] - Immunohistochemistry image showing positive staining of TNF alpha Variant Antibody (Z74P2C5*A12) in adipose tissue vaccinated rainbow trout. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required, which involves microwaving tissue for 20 minutes @ 800W in 10 mM citrate buffer (pH 6.0).")
![Immunohistochemistry: TNF-alpha Antibody (Z74P2C5*A12) - BSA Free [NBP2-61611] - Stained sections of fish intestine and liver of the control group showed negative staining reactions in all the examined parts of the intestinal and hepatic tissue (black and red arrows) against caspase-3 (NB600-1235) and TNF-alpha (NBP2-61611) antibodies. Nearly 10% of hepatic portal cells reacted positively to caspase-3. Scale bars = 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35624801/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-61611_mouse-tnf-alpha-mab-z74p2c5-a12-bsa-free-20920231412571.jpg)