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![Western Blot: Importin alpha 2/KPNA2 Antibody (9T3V8) [NBP3-16530] - Western blot analysis of extracts of various cell lines, using Importin alpha 2/KPNA2 Rabbit mAb (NBP3-16530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-9T3V8-Western-Blot-NBP3-16530-img0001.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody (9T3V8) [NBP3-16530] - Western blot analysis of extracts of various cell lines, using Importin alpha 2/KPNA2 Rabbit mAb (NBP3-16530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: TIA1 Antibody (TRIA1-1) [NBP3-27932] - Western blot testing of A) HepG2, B) PC3 and C) HeLa cell lysate wiht TIA1 antibody (clone TRIA1-1). The long and short forms differ by 17 amino acids. Predicted molecular weight: ~43kDa and ~15kDa (granulocyte-associated isoform).](http://images.novusbio.com/fullsize/nbp3-27932_mouse-tia1-mab-tria1-1-2711202419241317.jpg "Western Blot: TIA1 Antibody (TRIA1-1) [NBP3-27932] - Western blot testing of A) HepG2, B) PC3 and C) HeLa cell lysate wiht TIA1 antibody (clone TRIA1-1). The long and short forms differ by 17 amino acids. Predicted molecular weight: ~43kDa and ~15kDa (granulocyte-associated isoform).")
![Western Blot: TRIM5 alpha Antibody [NBP3-35735] - Western blot analysis of various lysates using TRIM5 alpha Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35735_rabbit-trim5-alpha-pab-1012202411381271.jpg "Western Blot: TRIM5 alpha Antibody [NBP3-35735] - Western blot analysis of various lysates using TRIM5 alpha Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: APOBEC3B Antibody [NBP2-99174] - Anti-APOBEC3B rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 50 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/APOBEC3B-Antibody-Western-Blot-NBP2-99174-img0002.jpg "Western Blot: APOBEC3B Antibody [NBP2-99174] - Anti-APOBEC3B rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 50 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: APOBEC3B Antibody [NBP2-99174] - APOBEC3B was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate4 ul anti-APOBEC3B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-APOBEC3B rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 46 kDa](http://images.novusbio.com/fullsize/APOBEC3B-Antibody-Immunoprecipitation-NBP2-99174-img0001.jpg)
![Immunohistochemistry: CD57 Antibody (PD00-21) [NBP3-32160] - Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue with Mouse anti-CD57 antibody (NBP3-32160) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32160) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32160_mouse-cd57-mab-pd00-21-298202414333690.jpg "Immunohistochemistry: CD57 Antibody (PD00-21) [NBP3-32160] - Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue with Mouse anti-CD57 antibody (NBP3-32160) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32160) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: NFATC3/NFAT4 Antibody [NBP2-04027] - Detection of human NFATc3 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04027 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04025 and NBP2-04026, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).](http://images.novusbio.com/fullsize/NFATC3-NFAT4-Antibody-Western-Blot-NBP2-04027-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NFATC3/NFAT4 Antibody [NBP2-04027] - Detection of human NFATc3 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04027 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04025 and NBP2-04026, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).")
![Immunoprecipitation: NFATC3/NFAT4 Antibody [NBP2-04026] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04025 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04026 and NBP2-04027, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/NFATC3-NFAT4-Antibody-Immunoprecipitation-NBP2-04026-img0001.jpg "Immunoprecipitation: NFATC3/NFAT4 Antibody [NBP2-04026] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04025 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04026 and NBP2-04027, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")
![Immunoprecipitation: NFATC3/NFAT4 Antibody [NBP2-04025] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04025 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04026 and NBP2-04027, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/NFATC3-NFAT4-Antibody-Immunoprecipitation-NBP2-04025-img0002.jpg "Immunoprecipitation: NFATC3/NFAT4 Antibody [NBP2-04025] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04025 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04026 and NBP2-04027, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")
![Western Blot: SNRPD1 Antibody - BSA Free [NBP3-45873] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (SNRPD1 antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-45873_rabbit-snrpd1-pab-bsa-free-28420251372077.jpg "Western Blot: SNRPD1 Antibody - BSA Free [NBP3-45873] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (SNRPD1 antibody) at dilution of 1:500")
![Western Blot: SerpinB9 Antibody - BSA Free [NBP3-45387] - K-562 cells were subjected to SDS PAGE followed by western blot with (SerpinB9 antibody) at dilution of 1:2000](http://images.novusbio.com/fullsize/nbp3-45387_rabbit-serpinb9-pab-bsa-free-28420251385053.jpg "Western Blot: SerpinB9 Antibody - BSA Free [NBP3-45387] - K-562 cells were subjected to SDS PAGE followed by western blot with (SerpinB9 antibody) at dilution of 1:2000")
