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![Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr142-Antibody-EM-54-Western-Blot-NBP2-37708-img0001.jpg "Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5")
![Western Blot: MARCO Antibody (PSH04-31) [NBP3-32568] - Western blot analysis of MARCO on different lysates with Rabbit anti-MARCO antibody (NBP3-32568) at 1/2,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Human lung tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 53 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32568) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32568_rabbit-marco-mab-psh04-31-278202416331272.jpg "Western Blot: MARCO Antibody (PSH04-31) [NBP3-32568] - Western blot analysis of MARCO on different lysates with Rabbit anti-MARCO antibody (NBP3-32568) at 1/2,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Human lung tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 53 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32568) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MARCO Antibody (PSH04-31) [NBP3-32568] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MARCO antibody (NBP3-32568) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32568) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32568_rabbit-marco-mab-psh04-31-298202414304115.jpg)
![Western Blot: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Western blot analysis of ICAM-2/CD102 on different lysates with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse heart tissue lysate (no heat) Lane 3: Mouse kidney tissue lysate (no heat) Lane 4: Mouse kidney tissue lysate Lane 5: Mouse spleen tissue lysate (no heat) Lane 6: Rat spleen tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 31 kDa Observed band size: 45-50 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32456) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32456_rabbit-icam-2-cd102-mab-je63-28-278202416285280.jpg "Western Blot: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Western blot analysis of ICAM-2/CD102 on different lysates with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse heart tissue lysate (no heat) Lane 3: Mouse kidney tissue lysate (no heat) Lane 4: Mouse kidney tissue lysate Lane 5: Mouse spleen tissue lysate (no heat) Lane 6: Rat spleen tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 31 kDa Observed band size: 45-50 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32456) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32456) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32456_rabbit-icam-2-cd102-mab-je63-28-298202414262629.jpg)
![Western Blot: IL-13 Antibody [NBP1-42776] - Analysis of recombinant swine IL-13 at 13.2kDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with , Inc. Anti-swine IL-13. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.](http://images.novusbio.com/fullsize/IL-13-Antibody-Western-Blot-NBP1-42776-img0004.jpg "Western Blot: IL-13 Antibody [NBP1-42776] - Analysis of recombinant swine IL-13 at 13.2kDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with , Inc. Anti-swine IL-13. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.")
![Western Blot: ICAM-2/CD102 Antibody [NBP1-31612] - Sample(30 ug whole cell lysate)A:HeLa S3 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Western-Blot-NBP1-31612-img0005.jpg "Western Blot: ICAM-2/CD102 Antibody [NBP1-31612] - Sample(30 ug whole cell lysate)A:HeLa S3 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ICAM-2/CD102 Antibody [NBP1-31612] - Paraffin-embedded breast cancer, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Immunohistochemistry-Paraffin-NBP1-31612-img0004.jpg)
![Western Blot: C8B Antibody [NBP2-15651] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/C8B-Antibody-Western-Blot-NBP2-15651-img0004.jpg "Western Blot: C8B Antibody [NBP2-15651] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:5000.")
![Immunohistochemistry-Paraffin: C8B Antibody [NBP2-15651] - Analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/C8B-Antibody-Immunohistochemistry-Paraffin-NBP2-15651-img0003.jpg)
![Immunohistochemistry-Paraffin: STIM2 Antibody [NBP2-44170] - Staining of human Kidney tissue:](http://images.novusbio.com/fullsize/STIM2-Antibody-Immunohistochemistry-Paraffin-NBP2-44170-img0001.jpg "Immunohistochemistry-Paraffin: STIM2 Antibody [NBP2-44170] - Staining of human Kidney tissue:")
![Western Blot: ILT2/CD85j/LILRB1 Antibody [NBP2-15014] - Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/ILT2-CD85j-LILRB1-Antibody-Western-Blot-NBP2-15014-img0002.jpg "Western Blot: ILT2/CD85j/LILRB1 Antibody [NBP2-15014] - Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE; antibody diluted at 1:10000.")
![LILRB1 antibody [N1C1] detects LILRB1 protein by immunofluorescent analysis.Sample: NK-92 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LILRB1 stained by LILRB1 antibody [N1C1] (NBP2-15014) diluted at 1:100.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15014_rabbit-polyclonal-ilt2-cd85j-lilrb1-antibody-immunocytochemistry-immunofluorescence-261120251971823.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LILRB1 antibody [N1C1] (NBP2-15014) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15014_rabbit-polyclonal-ilt2-cd85j-lilrb1-antibody-western-blot-2611202518345214.jpg)
![Western Blot: FMNL1 Antibody [NBP1-33431] - Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/FMNL1-Antibody-Western-Blot-NBP1-33431-img0003.jpg "Western Blot: FMNL1 Antibody [NBP1-33431] - Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: FMNL1 Antibody [NBP1-33431] - Paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/FMNL1-Antibody-Immunohistochemistry-Paraffin-NBP1-33431-img0002.jpg)
![Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (C1) [NBP3-41507] - DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 40ug/ml NBP3-41507; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41507_mouse-nkg2d-cd314-mab-c1-612202421121356.jpg "Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (C1) [NBP3-41507] - DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 40ug/ml NBP3-41507; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: NKG2D/CD314 Antibody (C1) [NBP3-41507] - Sample: Lane1: Rat Pancreas lysate; Lane2: Rat Spleen lysate Primary Ab: 1ug/ml NBP3-41507](http://images.novusbio.com/fullsize/nbp3-41507_mouse-nkg2d-cd314-mab-c1-6122024212716.jpg)
![Western Blot: T-bet/TBX21 Antibody [NBP2-20583] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-Western-Blot-NBP2-20583-img0003.jpg "Western Blot: T-bet/TBX21 Antibody [NBP2-20583] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:3000.")
![Immunohistochemistry-Paraffin: T-bet/TBX21 Antibody [NBP2-20583] - DLD-1 xenograft, using TBX21 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-Immunohistochemistry-Paraffin-NBP2-20583-img0004.jpg)