| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-62010B | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB120-7055 | |||
| HRP | NB120-7171 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP3-11660 | |||
| DyLight 680 | NBP3-11664 | |||
| DyLight 800 | NBP3-11663 | |||
| FITC | NBP3-11662 | |||
![Western Blot: Transcription factor E3 Antibody [H00007030-D01P] - Analysis of TFE3 expression in mouse kidney.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-Western-Blot-H00007030-D01P-img0003.jpg "Western Blot: Transcription factor E3 Antibody [H00007030-D01P] - Analysis of TFE3 expression in mouse kidney.")
![Western Blot: Transcription factor E3 Antibody [H00007030-D01P] - Analysis of TFE3 expression in transfected 293T cell line by TFE3 polyclonal antibody.Lane 1: TFE3 transfected lysate(61.50 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-Western-Blot-H00007030-D01P-img0004.jpg)
![ELISA: IgG3 Antibody (RM218) - Azide and BSA Free [NBP3-25954] - A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp3-25954_rabbit-igg3-mab-rm218-azide-and-bsa-free-2262024231104.png "ELISA: IgG3 Antibody (RM218) - Azide and BSA Free [NBP3-25954] - A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![ELISA: IgG3 Antibody (RM218) - Azide and BSA Free [NBP3-25954] - ELISA of mouse immunoglobulins shows RM218 reacts to both mouse IgG3 kappa and IgG3 lambda; No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp3-25954_rabbit-igg3-mab-rm218-azide-and-bsa-free-24620241332439.png)
![Western Blot: Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody [FITC] [NB120-7050] - Western blot of Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody [FITC].Lane 1: Rabbit IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody [FITC] at 1:1000 for 60 min at RT.Blocking: Fluorescent blocking buffer for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG.Other band(s): None.](http://images.novusbio.com/fullsize/Goat-F-ab-anti-Rabbit-IgG-H+L-Secondary-Antibody-FITC-Western-Blot-NB120-7050-img0005.jpg "Western Blot: Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody [FITC] [NB120-7050] - Western blot of Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody [FITC].Lane 1: Rabbit IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody [FITC] at 1:1000 for 60 min at RT.Blocking: Fluorescent blocking buffer for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG.Other band(s): None.")
![Western Blot: Rat Pure-Blot anti-Mouse IgG (H+L) Secondary Antibody (eB144) [HRP] [NBP3-11661] - Western Blot of Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP]. Lane 1: Mouse IgG, non-denatured. Lane 2: 5 uL Opal Pre-stained Ladder [HRP] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 160 kDa non-denatured for Mouse IgG.](http://images.novusbio.com/fullsize/Rat-Pure-Blot-anti-Mouse-IgG-Secondary-Antibody-eB144-HRP-Western-Blot-NBP3-11661-img0002.jpg "Western Blot: Rat Pure-Blot anti-Mouse IgG (H+L) Secondary Antibody (eB144) [HRP] [NBP3-11661] - Western Blot of Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP]. Lane 1: Mouse IgG, non-denatured. Lane 2: 5 uL Opal Pre-stained Ladder [HRP] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 160 kDa non-denatured for Mouse IgG.")
![Western Blot: Rat Pure-Blot anti-Mouse IgG (H+L) Secondary Antibody (eB144) [HRP] [NBP3-11661] - Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti-human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti-Caspase 7 using Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP] (Lane 1) or conventional HRP-conjugated anti-mouse antibody (Lane 2) - note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re-immunoblotted using conventional HRP-conjugated anti-mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP] detects only native antibody and not denatured heavy and light chains.](http://images.novusbio.com/fullsize/Rat-Pure-Blot-anti-Mouse-IgG-Secondary-Antibody-eB144-HRP-Western-Blot-NBP3-11661-img0001.jpg)
![Immunoprecipitation:AP1S1 AntibodyNBP3-28986] -Detection of human AP1S1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AP1S1 antibody NBP3-28986 (lot NBP3-28986-1) used for IP at 6 µg per reaction. For blotting immunoprecipitated AP1S1, NBP3-28986 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-28986_rabbit-ap1s1-pab-30122024182314.jpg "Immunoprecipitation:AP1S1 AntibodyNBP3-28986] -Detection of human AP1S1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AP1S1 antibody NBP3-28986 (lot NBP3-28986-1) used for IP at 6 µg per reaction. For blotting immunoprecipitated AP1S1, NBP3-28986 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: West Nile Virus C Protein Antibody [NBP3-13052] - Non-infected (-) and infected Vero whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with West Nile Virus C Protein C antibody (NBP3-13052).](http://images.novusbio.com/fullsize/West-Nile-Virus-C-Protein-Antibody-Western-Blot-NBP3-13052-img0006.jpg "Western Blot: West Nile Virus C Protein Antibody [NBP3-13052] - Non-infected (-) and infected Vero whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with West Nile Virus C Protein C antibody (NBP3-13052).")
![Western Blot: West Nile Virus C Protein Antibody [NBP3-13052] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with West Nile Virus C Protein C antibody (NBP3-13052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13052_rabbit-west-nile-virus-c-protein-pab-91020242014693.jpg)