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![Western Blot: Cytochrome b245 alpha Antibody [NBP3-13323] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with Cytochrome b245 alpha antibody (NBP3-13323) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cytochrome-b245-alpha-Antibody-Western-Blot-NBP3-13323-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Cytochrome b245 alpha Antibody [NBP3-13323] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with Cytochrome b245 alpha antibody (NBP3-13323) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Cytochrome b245 alpha Antibody [NBP3-13323] - Cytochrome b245 alpha antibody detects Cytochrome b245 alpha protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Cytochrome b245 alpha stained by Cytochrome b245 alpha antibody (NBP3-13323) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Cytochrome-b245-alpha-Antibody-Immunohistochemistry-Paraffin-NBP3-13323-img0001.jpg)
![Western Blot: Cytochrome b245 alpha Antibody [NBP1-59573] - Titration: 0.2-1 ug/ml, Positive Control: Human Small Intestine.](http://images.novusbio.com/fullsize/Cytochrome-b245-alpha-Antibody-Western-Blot-NBP1-59573-img0003.jpg "Western Blot: Cytochrome b245 alpha Antibody [NBP1-59573] - Titration: 0.2-1 ug/ml, Positive Control: Human Small Intestine.")
![Immunohistochemistry: Cytochrome b245 alpha Antibody [NBP1-59573] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Plasma membrane in intercalated disk Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize/Cytochrome-b245-alpha-Antibody-Immunohistochemistry-NBP1-59573-img0004.jpg)
![Western Blot: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:71 kDa](http://images.novusbio.com/fullsize/Serpin-G1-C1-Inhibitor-Antibody-18-Western-Blot-NBP2-89738-img0002.jpg "Western Blot: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:71 kDa")
![Immunoprecipitation: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-SERPING1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-SERPING1 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 55 kDa Observed band size: 100 kDa](http://images.novusbio.com/fullsize/Serpin-G1-C1-Inhibitor-Antibody-18-Immunoprecipitation-NBP2-89738-img0001.jpg)
![Western Blot: Adenosine A1R Antibody (10D12) [NBP3-15218] - Positive WB detected in: A549 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate. All lanes: Adenosine A1R antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37, 14 KDa. Observed band size: 37 KDa.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-10D12-Western-Blot-NBP3-15218-img0002.jpg "Western Blot: Adenosine A1R Antibody (10D12) [NBP3-15218] - Positive WB detected in: A549 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate. All lanes: Adenosine A1R antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37, 14 KDa. Observed band size: 37 KDa.")
![Immunohistochemistry: Adenosine A1R Antibody (10D12) [NBP3-15218] - IHC image of Adenosine A1R antibody diluted at 1:100 and staining in paraffin-embedded human brain tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-10D12-Immunohistochemistry-NBP3-15218-img0001.jpg)
![Western Blot: ACE/CD143 Antibody (ACE/3765) [NBP3-07780] - Western Blot Analysis of human Kidney tissue lysate using ACE/CD143 Mouse Monoclonal Antibody (ACE/3765).](http://images.novusbio.com/fullsize/ACE-CD143-Antibody-ACE-3765-Western-Blot-NBP3-07780-img0003.jpg "Western Blot: ACE/CD143 Antibody (ACE/3765) [NBP3-07780] - Western Blot Analysis of human Kidney tissue lysate using ACE/CD143 Mouse Monoclonal Antibody (ACE/3765).")
![Immunohistochemistry-Paraffin: ACE/CD143 Antibody (ACE/3765) [NBP3-07780] - Formalin-fixed, paraffin-embedded human small intestine stained with ACE/CD143 Mouse Monoclonal Antibody (ACE/3765).](http://images.novusbio.com/fullsize/ACE-CD143-Antibody-ACE-3765-Immunohistochemistry-Paraffin-NBP3-07780-img0002.jpg)
![ELISA: Mouse Serpin G1/C1 Inhibitor ELISA Kit (Colorimetric) [NBP2-70029] - Samples were spiked with high concentrations of Mouse Serpin G1/C1 Inhibitor and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70029_mouse-serpin-g1-c1-inhibitor-elisa-kit-colorimetric-132202416243492.jpg "ELISA: Mouse Serpin G1/C1 Inhibitor ELISA Kit (Colorimetric) [NBP2-70029] - Samples were spiked with high concentrations of Mouse Serpin G1/C1 Inhibitor and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Serpin G1/C1 Inhibitor ELISA Kit (Colorimetric) [NBP2-70029] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70029_mouse-serpin-g1-c1-inhibitor-elisa-kit-colorimetric-4320248582592.jpg)
![Western Blot: ACE/CD143 Antibody (1C9J3) [NBP3-15373] - Western blot analysis of extracts of various cell lines, using ACE/CD143 Rabbit mAb (NBP3-15373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ACE-CD143-Antibody-1C9J3-Western-Blot-NBP3-15373-img0001.jpg "Western Blot: ACE/CD143 Antibody (1C9J3) [NBP3-15373] - Western blot analysis of extracts of various cell lines, using ACE/CD143 Rabbit mAb (NBP3-15373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunoprecipitation: ACE/CD143 Antibody (1C9J3) [NBP3-15373] - Immunoprecipitation analysis of 600ug extracts of Mouse kidney cells using 3ug ACE/CD143 antibody (NBP3-15373). Western blot was performed from the immunoprecipitate using ACE/CD143 antibody (NBP3-15373) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/ACE-CD143-Antibody-1C9J3-Immunoprecipitation-NBP3-15373-img0002.jpg)
![Western Blot: COX-1 Antibody (6B6) [NBP3-26362] - Positive Western Blot detected in: HL60 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissue All lanes: COX-1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 69, 65, 62, 57, 72, 73 kDa Observed band size: 72 kDa](http://images.novusbio.com/fullsize/nbp3-26362_rabbit-cox-1-mab-6b6-282202411472131.jpg "Western Blot: COX-1 Antibody (6B6) [NBP3-26362] - Positive Western Blot detected in: HL60 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissue All lanes: COX-1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 69, 65, 62, 57, 72, 73 kDa Observed band size: 72 kDa")
![Flow Cytometry: COX-1 Antibody (6B6) [NBP3-26362] - Overlay histogram showing Hela cells stained with COX-1 Antibody (6B6) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26362_rabbit-cox-1-mab-6b6-28220241418366.jpg)
![Western Blot: COX1 Antibody [NBP1-62466] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/COX1-Antibody-Western-Blot-NBP1-62466-img0002.jpg "Western Blot: COX1 Antibody [NBP1-62466] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: COX-1 Antibody (8L4X10) [NBP3-16214] - Western blot analysis of extracts of various cell lines, using COX-1 Rabbit mAb (NBP3-16214) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/COX-1-Antibody-8L4X10-Western-Blot-NBP3-16214-img0001.jpg "Western Blot: COX-1 Antibody (8L4X10) [NBP3-16214] - Western blot analysis of extracts of various cell lines, using COX-1 Rabbit mAb (NBP3-16214) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: Serpin G1/C1 Inhibitor Antibody (0D3W8) [NBP3-15721] - Western blot analysis of extracts of various cell lines, using Serpin G1/C1 Inhibitor antibody (NBP3-15721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Serpin-G1-C1-Inhibitor-Antibody-0D3W8-Western-Blot-NBP3-15721-img0001.jpg "Western Blot: Serpin G1/C1 Inhibitor Antibody (0D3W8) [NBP3-15721] - Western blot analysis of extracts of various cell lines, using Serpin G1/C1 Inhibitor antibody (NBP3-15721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")