| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89345-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90405-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89730-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90272-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89323-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06087-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89841-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90530-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90096-50ul | |||
![Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-002-Flow-Cytometry-NBP2-89345-img0001.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Leptin/OB Antibody (002) [NBP2-89345] - Staining of LEP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-89345 (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Cytoplasm .](http://images.novusbio.com/fullsize/nbp2-89345_rabbit-monoclonal-leptin-ob-antibody-002-23520251129371.png)
![Flow Cytometry: Fc gamma RI/CD64 Antibody (027) [NBP2-90405] - Flow cytometric analysis of Mouse Fc gamma RI/CD64 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse Fc gamma RI/CD64, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Fc-gamma-RI-CD64-Antibody-027-Flow-Cytometry-NBP2-90405-img0001.jpg "Flow Cytometry: Fc gamma RI/CD64 Antibody (027) [NBP2-90405] - Flow cytometric analysis of Mouse Fc gamma RI/CD64 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse Fc gamma RI/CD64, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: Fc gamma RI/CD64 Antibody (027) [NBP2-90405] - Immunofluorescence staining of mouse Fc gamma RI/CD64 in Raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90405 antibody (1:60) at 37C 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.](http://images.novusbio.com/fullsize/nbp2-90405_rabbit-monoclonal-fc-gamma-ri-cd64-antibody-027-23520252135127.png)
![Western Blot: CD3 delta Antibody (138) [NBP2-89730] - Lane A: Jurkat Whole Cell Lysate Lane B: Hu-T78 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:19 kDa Observed band size:24 kDa](http://images.novusbio.com/fullsize/CD3-delta-Antibody-138-Western-Blot-NBP2-89730-img0002.jpg "Western Blot: CD3 delta Antibody (138) [NBP2-89730] - Lane A: Jurkat Whole Cell Lysate Lane B: Hu-T78 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:19 kDa Observed band size:24 kDa")
![Immunoprecipitation: CD3 delta Antibody (138) [NBP2-89730] - Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg HuT78 Whole Cell LysateLane C:0.5 mg Caco-2 Whole Cell Lysate 0.5 uL anti-CD3D rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-CD3D rabbit monoclonal antibody,at 1:250 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 20 kDa Observed band size: 20 kDa](http://images.novusbio.com/fullsize/CD3-delta-Antibody-138-Immunoprecipitation-NBP2-89730-img0001.jpg)
![Western Blot: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-145-Western-Blot-NBP2-90272-img0001.jpg "Western Blot: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:15 kDa")
![Immunoprecipitation: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate2 uL anti-AIF1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AIF1 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-145-Immunoprecipitation-NBP2-90272-img0002.jpg)
![Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (003) [NBP2-89323] - Lane A: Jurkat Whole Cell Lysate Lane B: K562 Whole Cell LysateLane C: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:23 kDa](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-003-Western-Blot-NBP2-89323-img0001.jpg "Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (003) [NBP2-89323] - Lane A: Jurkat Whole Cell Lysate Lane B: K562 Whole Cell LysateLane C: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:23 kDa")
![<b>Genetic Strategies Validation. </b>NBP2-89323 used at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: GSTP1 knockout Hela Whole Cell Lysate. Lysates loaded at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)[HRP] used at at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 23 kDa.](http://images.novusbio.com/fullsize/nbp2-89323_rabbit-monoclonal-esam-antibody-202-45202316595485.png)
![Western Blot: Kininogen Antibody [NBP2-87732] - Host: Rabbit. Target Name: Kininogen. Sample Type: COLO205 Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/LMW-Kininogen-Antibody-Western-Blot-NBP2-87732-img0001.jpg "Western Blot: Kininogen Antibody [NBP2-87732] - Host: Rabbit. Target Name: Kininogen. Sample Type: COLO205 Whole Cell lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: Latexin Antibody (8E1G6C1D4) [NBP3-06087] - Anti-LXN mouse monoclonal antibody at 1:500 dilution Lane A: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 115000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa Observed band size:26 kDa](http://images.novusbio.com/fullsize/Latexin-Antibody-8E1G6C1D4-Western-Blot-NBP3-06087-img0001.jpg "Western Blot: Latexin Antibody (8E1G6C1D4) [NBP3-06087] - Anti-LXN mouse monoclonal antibody at 1:500 dilution Lane A: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 115000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa Observed band size:26 kDa")
![Immunoprecipitation: Latexin Antibody (8E1G6C1D4) [NBP3-06087] - LXN was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate 0.5 uL anti-LXN mouse monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LXN mouse monoclonal antibody, at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 25.7 kDa Observed band size: 27 kDa](http://images.novusbio.com/fullsize/Latexin-Antibody-8E1G6C1D4-Immunoprecipitation-NBP3-06087-img0002.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (027) [NBP2-89841] - Analysis of Human HPGD expression on Jurkat cells. The cells were treated, stained with purified anti-Human HPGD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-027-Flow-Cytometry-NBP2-89841-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: 15-PGDH/HPGD Antibody (027) [NBP2-89841] - Analysis of Human HPGD expression on Jurkat cells. The cells were treated, stained with purified anti-Human HPGD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody (027) [NBP2-89841] - Staining of Human HPGD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89841 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89841_rabbit-monoclonal-15-pgdh-hpgd-antibody-027-23520252135140.png)
![Western Blot: Nectin-2/CD112 Antibody (110) [NBP2-90530] - Anti-Carbonic Anhydrase XIV/CA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Membrane Lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa](http://images.novusbio.com/fullsize/Nectin-2-CD112-Antibody-110-Western-Blot-NBP2-90530-img0003.jpg "Western Blot: Nectin-2/CD112 Antibody (110) [NBP2-90530] - Anti-Carbonic Anhydrase XIV/CA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Membrane Lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa")
![Immunoprecipitation: Nectin-2/CD112 Antibody (110) [NBP2-90530] - Mouse Nectin-2/CD112 was immunoprecipitated using: Lane A: 0.5 mg Raji Whole Cell Lysate. 0.5 ul anti-Mouse Nectin-2/CD112 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Nectin-2/CD112 rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa](http://images.novusbio.com/fullsize/Nectin-2-CD112-Antibody-110-Immunoprecipitation-NBP2-90530-img0004.jpg)
![Western Blot: CD58/LFA-3 Antibody (085) [NBP2-90096] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDaObserved band size:61 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-085-Western-Blot-NBP2-90096-img0001.jpg "Western Blot: CD58/LFA-3 Antibody (085) [NBP2-90096] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDaObserved band size:61 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: CD58/LFA-3 Antibody (085) [NBP2-90096] - Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-CD58 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD58 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 61 kDa](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-085-Immunoprecipitation-NBP2-90096-img0002.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP3-30965] - Immunohistochemical staining of FFPE human colon carcinoma tissue using Dicer antibody (Cat. No. NBP3-30965). Antibody used at 1 ug/ml and visualized using DAB. Pathologists Comments: Cytoplasmic staining on mucous cells.](http://images.novusbio.com/fullsize/nbp3-30965_rabbit-dicer-pab-811202420592150.jpg "Immunohistochemistry-Paraffin: Dicer Antibody [NBP3-30965] - Immunohistochemical staining of FFPE human colon carcinoma tissue using Dicer antibody (Cat. No. NBP3-30965). Antibody used at 1 ug/ml and visualized using DAB. Pathologists Comments: Cytoplasmic staining on mucous cells.")
