| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61708-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20109-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52657-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11996-0.025mg | |||
![Western Blot: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (3B8B10) [NBP2-61708] - Analysis using B3GAT1 mAb against HEK293 (1) and B3GAT1 (AA: 193-334)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/beta-1-3-Glucuronyltransferase-1-B3GAT1-Antibody-3B8B10-Western-Blot-NBP2-61708-img0004.jpg "Western Blot: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (3B8B10) [NBP2-61708] - Analysis using B3GAT1 mAb against HEK293 (1) and B3GAT1 (AA: 193-334)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (3B8B10) [NBP2-61708] - Analysis of Hela cells using B3GAT1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/beta-1-3-Glucuronyltransferase-1-B3GAT1-Antibody-3B8B10-Flow-Cytometry-NBP2-61708-img0002.jpg)
![Western Blot: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (3B8B10) [NBP2-61708] - Analysis using B3GAT1 mAb against human B3GAT1 (AA: 193-334) recombinant protein. (Expected MW is 41.5 kDa)](http://images.novusbio.com/fullsize/beta-1-3-Glucuronyltransferase-1-B3GAT1-Antibody-3B8B10-Western-Blot-NBP2-61708-img0003.jpg)
![Western Blot: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (3B8B10) [NBP2-61708] - Analysis using B3GAT1 mouse mAb against MOLT4 (1), Raji (2), and Ramos (3) cell lysate.](http://images.novusbio.com/fullsize/beta-1-3-Glucuronyltransferase-1-B3GAT1-Antibody-3B8B10-Western-Blot-NBP2-61708-img0005.jpg)
![ELISA: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (3B8B10) [NBP2-61708] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/beta-1-3-Glucuronyltransferase-1-B3GAT1-Antibody-3B8B10-ELISA-NBP2-61708-img0001.jpg)
![Immunohistochemistry-Paraffin: CEACAM-5/CD66e Antibody (CB30) [NB110-58734] - Human colorectal carcinoma](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-CB30-Immunohistochemistry-Paraffin-NB110-58734-img0002.jpg "Immunohistochemistry-Paraffin: CEACAM-5/CD66e Antibody (CB30) [NB110-58734] - Human colorectal carcinoma")
![Immunohistochemistry-Paraffin: CEACAM-5/CD66e Antibody (CB30) [NB110-58734] - Human colorectal carcinoma](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-CB30-Immunohistochemistry-Paraffin-NB110-58734-img0001.jpg)
![Western Blot: CD53 Antibody (JE60-38) [NBP3-32159] - Western blot analysis of CD53 on different lysates with Rabbit anti-CD53 antibody (NBP3-32159) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 35-42 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32159) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32159_rabbit-cd53-mab-je60-38-278202416331213.jpg "Western Blot: CD53 Antibody (JE60-38) [NBP3-32159] - Western blot analysis of CD53 on different lysates with Rabbit anti-CD53 antibody (NBP3-32159) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 35-42 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32159) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD53 Antibody (JE60-38) [NBP3-32159] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD53 antibody (NBP3-32159) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32159) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32159_rabbit-cd53-mab-je60-38-298202414333654.jpg)
![Immunocytochemistry/ Immunofluorescence: CD53 Antibody (JE60-38) [NBP3-32159] - Immunocytochemistry analysis of Jurkat cells labeling CD53 with Rabbit anti-CD53 antibody (NBP3-32159) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD53 antibody (NBP3-32159) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32159_rabbit-cd53-mab-je60-38-29202410334023.jpg)
![Immunohistochemistry-Paraffin: XCR1/CCXCR1 Antibody [NLS156] - Colon, Carcinoma](http://images.novusbio.com/fullsize/XCR1-CCXCR1-Antibody-Immunohistochemistry-Paraffin-NLS156-img0006.jpg "Immunohistochemistry-Paraffin: XCR1/CCXCR1 Antibody [NLS156] - Colon, Carcinoma")
![Immunohistochemistry-Paraffin: XCR1/CCXCR1 Antibody [NLS156] - Analysis of anti-XCR1 antibody with human spleen at 2.5 ug/ml.](http://images.novusbio.com/fullsize/XCR1-CCXCR1-Antibody-Immunohistochemistry-NLS156-img0002.jpg)
![Immunohistochemistry-Paraffin: XCR1/CCXCR1 Antibody [NLS156] - Skin, Malignant Melanoma](http://images.novusbio.com/fullsize/XCR1-CCXCR1-Antibody-Immunohistochemistry-Paraffin-NLS156-img0004.jpg)
![Immunohistochemistry-Paraffin: XCR1/CCXCR1 Antibody [NLS156] - Ovary, carcinoma](http://images.novusbio.com/fullsize/XCR1-CCXCR1-Antibody-Immunohistochemistry-Paraffin-NLS156-img0005.jpg)
![Western Blot: CYBB/NOX2 Antibody [NBP1-59062] - THP-1 cell lysate, Antibody Titration: 1.0ug/ml](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-Western-Blot-NBP1-59062-img0004.jpg "Western Blot: CYBB/NOX2 Antibody [NBP1-59062] - THP-1 cell lysate, Antibody Titration: 1.0ug/ml")
![Immunohistochemistry-Paraffin: CYBB/NOX2 Antibody [NBP1-59062] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-Immunohistochemistry-Paraffin-NBP1-59062-img0003.jpg)
![Western Blot: CYBB/NOX2 Antibody [NBP1-59062] - MCF7 tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-Western-Blot-NBP1-59062-img0001.jpg)
![Immunohistochemistry-Paraffin: CYBB/NOX2 Antibody [NBP1-59062] - Human Spleen](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-Immunohistochemistry-Paraffin-NBP1-59062-img0005.jpg)
![Western Blot: HLA Class I Antibody [NBP2-75928] - Western blot analysis of HLA Class I on Hela cell lysate using anti-HLA Class I antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/HLA-Class-I-Antibody-Western-Blot-NBP2-75928-img0008.jpg "Western Blot: HLA Class I Antibody [NBP2-75928] - Western blot analysis of HLA Class I on Hela cell lysate using anti-HLA Class I antibody at 1/1,000 dilution.")
![Flow Cytometry: HLA Class I Antibody [NBP2-75928] - analysis of Jurkat cells with HLA Class 1 ABC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/HLA-Class-I-Antibody-Flow-Cytometry-NBP2-75928-img0007.jpg)
![Immunohistochemistry-Paraffin: HLA Class I Antibody [NBP2-75928] - analysis of paraffin-embedded human colon cancer tissue using anti-HLA Class 1 ABC antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HLA-Class-I-Antibody-Immunohistochemistry-Paraffin-NBP2-75928-img0003.jpg)
![Western Blot: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Western blot analysis of CD43/Sialophorin on different lysates with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: U-937 cell lysate Lane 5: HT-29 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 100-130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32154) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-278202415212446.jpg "Western Blot: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Western blot analysis of CD43/Sialophorin on different lysates with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: U-937 cell lysate Lane 5: HT-29 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 100-130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32154) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Immunohistochemical analysis of paraffin-embedded human B lymphocyte tumor tissue with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32154) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-298202414161888.jpg)
![Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Immunocytochemistry analysis of U-937 (positive) and HT-29 (negative) labeling CD43/Sialophorin with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-2920241114826.jpg)
![Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.](http://images.novusbio.com/fullsize/Complement-C4d-Antibody-Immunohistochemistry-Paraffin-NBP2-31130-img0003.jpg "Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.")
![Immunohistochemistry: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of human kidney](http://images.novusbio.com/fullsize/Complement-C4d-Antibody-Immunohistochemistry-NBP2-31130-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.](http://images.novusbio.com/fullsize/Complement-C4d-Antibody-Immunohistochemistry-Paraffin-NBP2-31130-img0002.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0005.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].")
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the palatine tonsil using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0003.jpg)
![Western Blot: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - HeLa(A) (0.03ug/ml), MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml), human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-43B-8 (NBP3-20109) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924288.png "Western Blot: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - HeLa(A) (0.03ug/ml), MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml), human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-43B-8 (NBP3-20109) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-43B-8 (NBP3-20109) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20109, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924248.png)
![Flow Cytometry: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-43B-8 (NBP3-20109, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924262.png)
![Flow Cytometry: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) - Chimeric [NBP2-52657] - Flow-cytometry using the anti-CD52 research biosimilar antibody Campath-1G (YTH 34.5-G2b) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1G / YTH 34.5-G2b (NBP2-52657, panel B) at a concentration of 0.25 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD52-Antibody-YTH-34-5-G2b-Campath-1G-Chimeric-Flow-Cytometry-NBP2-52657-img0001.jpg "Flow Cytometry: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) - Chimeric [NBP2-52657] - Flow-cytometry using the anti-CD52 research biosimilar antibody Campath-1G (YTH 34.5-G2b) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1G / YTH 34.5-G2b (NBP2-52657, panel B) at a concentration of 0.25 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Western Blot: CCR5 [p Ser337] Antibody (V14/2) - Chimeric [NBP3-11996] - K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of V14/2 (NBP3-11996) at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CCR5-p-Ser337-Antibody-V14-2-Chimeric-Western-Blot-NBP3-11996-img0001.jpg "Western Blot: CCR5 [p Ser337] Antibody (V14/2) - Chimeric [NBP3-11996] - K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of V14/2 (NBP3-11996) at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CCR5 [p Ser337] Antibody (V14/2) - Chimeric [NBP3-11996] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of V14/2 (NBP3-11996) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11996, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CCR5-p-Ser337-Antibody-V14-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11996-img0002.jpg)
![Flow Cytometry: CCR5 [p Ser337] Antibody (V14/2) - Chimeric - Azide and BSA Free [NBP3-11996] - U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-11996 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-11996_rabbit-monoclonal-ccr5-p-ser337-antibody-v14-2-chimeric-74202522113035.png)