| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| PE | NBP2-27252 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25229 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18576-25ul | |||
| Azide and BSA Free | NBP3-44177 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24397SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49891-0.1mg | |||
| BSA Free | NBP2-49891-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33150 | |||
| IHC-Prediluted | NBP2-44388 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54582-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38520-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38479-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18562-25ul | |||
| Azide and BSA Free | NBP3-43932 | |||
![Western Blot: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis of IL-17A in 1) human, 2) mouse and 3) rat full-length recombinant IL-17A protein using NBP2-27338 at 1 ug/ml.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-4H1524-Western-Blot-NBP2-27338-img0004.jpg "Western Blot: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis of IL-17A in 1) human, 2) mouse and 3) rat full-length recombinant IL-17A protein using NBP2-27338 at 1 ug/ml.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis using the PE conjugate of NBP2-25229. IL-17A positive cells in PMA/IM stimulated fresh PBMC: PBMC stimulated for 6h with PMA (25ng/ml) & IM (250ng/ml), in the presence of BFA. Cells were surface stained with CD4 APC, fixed & permeabilised for intracellular staining with PE conjugated](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-4H1524-Flow-Cytometry-NBP2-27338-img0007.jpg)
![Block/Neutralize: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis using Azide Free version of NBP2-27338. Neutralization by Human IL-17A Antibody. Secretion of IL-6 by 3T3 cells stimulated with recombinant human IL-17A (20ng/ml) is neutralized by increasing concentrations of IL-17A monoclonal antibody. Antibody](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-4H1524-Block-Neutralize-NBP2-27338-img0006.jpg)
![Western Blot: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Analysis in human cell line SK-MEL-30 and human cell line U-2 OS.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Western-Blot-NBP3-18576-img0007.jpg "Western Blot: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Analysis in human cell line SK-MEL-30 and human cell line U-2 OS.")
![Immunocytochemistry/Immunofluorescence: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of SK-MEL-30 cells using the Anti-MART1 monoclonal antibody, showing specific staining in endoplasmic reticulum in green. Nuclear probes are visualized in blue (where available).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunocytochemistry-Immunofluorescence-NBP3-18576-img0009.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunohistochemistry-Paraffin-NBP3-18576-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of SK-MEL-30 cells using the Anti-MART1 monoclonal antibody, showing specific staining in endoplasmic reticulum in green. Nuclear probes are visualized in blue (where available).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunocytochemistry-Immunofluorescence-NBP3-18576-img0001.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Analysis in human skin and prostate tissues using NBP3-18576 antibody. Corresponding MLANA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunohistochemistry-Paraffin-NBP3-18576-img0002.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of human skin shows strong cytoplasmic positivity in melanocytes.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunohistochemistry-Paraffin-NBP3-18576-img0003.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of human kidney shows no positivity as expected.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunohistochemistry-Paraffin-NBP3-18576-img0004.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of human cervix shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunohistochemistry-Paraffin-NBP3-18576-img0005.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NBP2-24397] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using FOXP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-Paraffin-NBP2-24397-img0002.jpg "Immunohistochemistry-Paraffin: FoxP3 Antibody [NBP2-24397] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using FOXP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.")
![Western Blot: FoxP3 Antibody [NBP2-24397] - Analysis of FOXP3 in (A) FOXP3 transfected and (B) mock transfected cell lysate using NBP2-24397 at 5 ug/ml.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Western-Blot-NBP2-24397-img0003.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649 and Mouse IgG1 FITC isotype control. Figure B. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a PE and Mouse anti Canine CD3 FITC.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0009.jpg "Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649 and Mouse IgG1 FITC isotype control. Figure B. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a PE and Mouse anti Canine CD3 FITC.")
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A: APC conjugated rat anti-Canine CD4 and FITC conjugated mouse IgG1 isotype control. Figure B: APC conjugated rat anti-Canine CD4 and FITC conjugated mouse anti-Canine CD3.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0001.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A: RPE conjugated Rat anti-Dog CD4 and Mouse IgG1 isotype control labeled with Goat anti-Mouse FITC. Figure B: RPE conjugated Rat anti-Dog CD4 and Mouse anti-Dog CD3 labeled with Goat anti Mouse DyLight FITC.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0002.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. Alexa Fluor 700 conjugated Rat anti Canine CD8 and FITC conjugated Mouse IgG1 isotype control. Figure B. Alexa Fluor 700 conjugated Rat anti Canine CD8 and FITC conjugated Mouse anti Canine CD3.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0003.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG2a isotype control. Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD4.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0004.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse IgG1 isotype control. Figure B. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse anti Canine CD3.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0005.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. RPE conjugated Rat anti Canine CD8 and FITC conjugated Mouse IgG1 isotype control. Figure B. RPE conjugated Rat anti Canine CD8 and FITC conjugated Mouse anti Canine CD3.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0006.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG1 isotype control. Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD8.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0007.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. FITC conjugated Mouse anti Canine CD3 and purified Rat IgG2a isotype control detected with Goat anti Rat IgG2a DyLight 649. Figure B. FITC conjugated Mouse anti Canine CD3 and purified Mouse anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0008.jpg)
![Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] - IL-17/IL-17A Antibody (TC11-18H10) [NBP1-72027] - Treatment with HBOT or PFD decreased IL-17A expression in the AD lesions. IHC for IL-17A on mouse skin. Scale bar is 100 um. The number of IL-17A-expressing cells was counted in five high-power fields (HPF) chosen at random in each slide by three different pathologists. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0109297) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-TC11-18H10-Immunohistochemistry-Paraffin-NBP1-72027-img0003.jpg "Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] - IL-17/IL-17A Antibody (TC11-18H10) [NBP1-72027] - Treatment with HBOT or PFD decreased IL-17A expression in the AD lesions. IHC for IL-17A on mouse skin. Scale bar is 100 um. The number of IL-17A-expressing cells was counted in five high-power fields (HPF) chosen at random in each slide by three different pathologists. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0109297) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) [NBP1-72027] - Analysis of anti-IL-17 antibody of mouse spleen at concentration 10 ug/ml.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-TC11-18H10-Immunohistochemistry-Paraffin-NBP1-72027-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] - TG2-dependent chemokine production in keratinocytes is critical for IL-17-producing CCR6+ gamma δT-cell & neutrophil dermal infiltration.a, b Effect of DMSO- or IMQ-treated WT- or TG2−/−-CM on CCR6+ gamma δT-cell (a) & CD11b+ Ly-6G+ cell (b) migration according to Transwell migration assays. Flow cytometric analysis of the percentage of cells migrating toward the CM in the presence or absence of anti-CXCL1 (a) & anti-CCL20 (b) antibodies (n = 3/group). c, d WT & TG2−/− mouse-derived CCR6+ gamma δT-cell (c) & CD11b+ Ly-6G+ cell (d) migration in response to recombinant mouse CCL20 & CXCL1, respectively. Data represent the mean ± SEM (n = 3/group). *p < 0.05; **p < 0.01. e, f Cells were isolated from the back skin of WT & TG2−/− mice treated with IMQ for four consecutive days & analyzed by flow cytometry. Representative flow cytometric profiles of CCR6+  gamma δTCRlow cells (e) & CD11bhigh Ly-6G+ cells (f). The percentage of cells is shown. Data represent the mean ± SEM (n = 9/group). g Skin sections were immunostained with CCR6 (green)- & IL-17 (red)-specific antibodies. Nuclei were stained with DAPI (blue). Scale bar, 100 μm. h–j Back skin was separated into epidermis & dermis & analyzed by RT-PCR for IL-17-producing gamma δT-cell markers (h) (Ccr6 & Il23r), IL17 & related cytokines (i) (Il17a, Il17f, & Il22), & myeloid cell-derived cytokines (j) (Il12a & Il23a). Data represent the mean ± SEM (n = 11/group). *p < 0.05; **p < 0.01 vs. WT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32355189), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-72027_rat-il-17-il-17a-mab-tc11-18h10-azide-and-bsa-free-310202416171471.jpg)
![Western Blot: Melan-A/MART-1 Antibody (M2-9E3) [NBP2-15198] - Western Blot Analysis of COLO38 cell lysate using Melan-A/MART-1 Antibody (M2-9E3).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-9E3-Western-Blot-NBP2-15198-img0005.jpg "Western Blot: Melan-A/MART-1 Antibody (M2-9E3) [NBP2-15198] - Western Blot Analysis of COLO38 cell lysate using Melan-A/MART-1 Antibody (M2-9E3).")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) [NBP2-15198] - Formalin-paraffin human melanoma stained with Melan-A Ab (M2-9E3). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-9E3-Immunohistochemistry-Paraffin-NBP2-15198-img0004.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) [NBP2-15198] - Formalin-paraffin human melanoma stained with Melan-A Ab (M2-9E3). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-9E3-Immunohistochemistry-Paraffin-NBP2-15198-img0003.jpg)
![Flow Cytometry: CD3 Antibody (13) [NBP2-43674] - Analysis of B6 / spleen. Blue: Isotype control (anti-mouse IgG-FITC) dilution: 1:300. Red: CD3 antibody dilution: 1:50.](http://images.novusbio.com/fullsize/CD3-Antibody-13-Flow-Cytometry-NBP2-43674-img0001.jpg "Flow Cytometry: CD3 Antibody (13) [NBP2-43674] - Analysis of B6 / spleen. Blue: Isotype control (anti-mouse IgG-FITC) dilution: 1:300. Red: CD3 antibody dilution: 1:50.")
![Flow Cytometry: CD3 Antibody (13) [NBP2-43674] - Analysis of Jurkat cell Black: Isotype control dilution: 1:50. Green: CD3 antibody dilution: 1:50.](http://images.novusbio.com/fullsize/CD3-Antibody-13-Flow-Cytometry-NBP2-43674-img0002.jpg)
![CD3 antibody [GT0013] detects CD3 protein at cell membrane by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD3 protein stained by CD3 antibody [GT0013] (NBP2-43674) diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43674_mouse-monoclonal-cd3-antibody-13-immunocytochemistry-immunofluorescence-2611202518272310.jpg)
![Immunocytochemistry/Immunofluorescence: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Immunofluorescence staining of Ramos cells using CD27/TNFRSF7 Antibody (LPFS2/1611) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunocytochemistry-Immunofluorescence-NBP2-53196-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Immunofluorescence staining of Ramos cells using CD27/TNFRSF7 Antibody (LPFS2/1611) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.")
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Formalin-fixed, paraffin-embedded human Colon stained with CD27/TNFRSF7 Antibody (LPFS2/1611).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunohistochemistry-Paraffin-NBP2-53196-img0006.jpg)
![Flow Cytometry: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Flow Cytometric Analysis of Ramos cells using CD27/TNFRSF7 Mouse Monoclonal Antibody (LPFS2/1611) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Flow-Cytometry-NBP2-53196-img0008.jpg)
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD27 Monoclonal Antibody (LPFS2/1611).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunohistochemistry-Paraffin-NBP2-53196-img0001.jpg)
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD27/TNFRSF7 Antibody (LPFS2/1611).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunohistochemistry-Paraffin-NBP2-53196-img0004.jpg)
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Formalin-fixed, paraffin-embedded human Spleen stained with CD27/TNFRSF7 Antibody (LPFS2/1611).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunohistochemistry-Paraffin-NBP2-53196-img0005.jpg)
![Protein Array: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CD27/TNFRSF7 Antibody (LPFS2/1611) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Protein-Array-NBP2-53196-img0007.jpg)
![Western Blot: FoxP3 Antibody (BLR034F) [NBP3-14670] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Rabbit anti-FOXP3 recombinant monoclonal antibody (NBP3-14670 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Western-Blot-NBP3-14670-img0001.jpg "Western Blot: FoxP3 Antibody (BLR034F) [NBP3-14670] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Rabbit anti-FOXP3 recombinant monoclonal antibody (NBP3-14670 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunohistochemistry: FoxP3 Antibody (BLR034F) [NBP3-14670] - FFPE section of human breast carcinoma. Antibody: Rabbit anti-FOXP3recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NBP3-14670-img0006.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody (BLR034F) [NBP3-14670] - Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NBP3-14670-img0003.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody (BLR034F) [NBP3-14670] - Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NBP3-14670-img0004.jpg)
![Immunohistochemistry: FoxP3 Antibody (BLR034F) [NBP3-14670] - Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NBP3-14670-img0005.jpg)
![Immunoprecipitation: FoxP3 Antibody (BLR034F) [NBP3-14670] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXP3 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. FOXP3 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated FOXP3, was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunoprecipitation-NBP3-14670-img0002.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38520] - Staining of human cerebral cortex, lymph node, spleen and tonsil using Anti-CD3E antibody NBP2-38520 (A) shows similar protein distribution across tissues to independent antibody NBP2-38479 (B).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38520-img0013.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38520] - Staining of human cerebral cortex, lymph node, spleen and tonsil using Anti-CD3E antibody NBP2-38520 (A) shows similar protein distribution across tissues to independent antibody NBP2-38479 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38520] - Staining in human lymph node and pancreas tissues . Corresponding CD3E RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38520-img0012.jpg)
![Western Blot: CD3 epsilon Antibody [NBP2-38520] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Western-Blot-NBP2-38520-img0002.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38520] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38520-img0015.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38520] - Staining of human granular layer of the cerebellum shows no cytoplasmic positivity as expected.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38520-img0007.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38520] - Staining of human lymph node shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38520-img0008.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38520] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38520-img0009.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38520] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38520-img0010.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38520] - Staining of human spleen shows moderate to strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38520-img0011.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38520] - Staining of human tonsil.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38520-img0014.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38479] - Staining in human lymph node and small intestine tissues . Corresponding CD3E RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38479-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38479] - Staining in human lymph node and small intestine tissues . Corresponding CD3E RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38479] - Staining of human cerebral cortex, lymph node, spleen and tonsil using Anti-CD3E antibody NBP2-38479 (A) shows similar protein distribution across tissues to independent antibody NBP2-38520 (B).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38479-img0012.jpg)
![Western Blot: CD3 epsilon Antibody [NBP2-38479] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Western-Blot-NBP2-38479-img0002.jpg)
![Western Blot: CD3 epsilon Antibody [NBP2-38479] - Staining of human spleen shows weak to moderate cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Western-Blot-NBP2-38479-img0010.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38479] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38479-img0013.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38479] - Staining of human lymph node shows weak to moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38479-img0007.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38479] - Staining of human purkinje layer of the cerebellum shows no cytoplasmic positivity as expected.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38479-img0008.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38479] - Staining of human small intestine shows weak to moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38479-img0009.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-38479] - Staining of human tonsil.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-38479-img0014.jpg)
![Western Blot: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Analysis in human cell line SK-MEL-30 and human cell line U-2 OS.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12864-Western-Blot-NBP3-18562-img0007.jpg "Western Blot: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Analysis in human cell line SK-MEL-30 and human cell line U-2 OS.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12864-Immunohistochemistry-Paraffin-NBP3-18562-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Staining of SK-MEL-30 cells using the Anti-MLANA monoclonal antibody, showing specific staining in endoplasmic reticulum in green. Nuclear probes are visualized in blue (where available).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12864-Immunocytochemistry-Immunofluorescence-NBP3-18562-img0001.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Analysis in human skin and prostate tissues using NBP3-18562 antibody. Corresponding MLANA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12864-Immunohistochemistry-Paraffin-NBP3-18562-img0002.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Staining of human skin shows strong cytoplasmic positivity in melanocytes.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12864-Immunohistochemistry-Paraffin-NBP3-18562-img0003.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Staining of human kidney shows no positivity as expected.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12864-Immunohistochemistry-Paraffin-NBP3-18562-img0004.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Staining of human cervix shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12864-Immunohistochemistry-Paraffin-NBP3-18562-img0005.jpg)