| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35783-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38604-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38538-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37388-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61725-0.025ml | |||
![Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Western-Blot-NBP3-15310-img0003.jpg "Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: CD133 Antibody (3Y4A5) [NBP3-15310] - Immunohistochemistry of paraffin-embedded human colon carcinoma using CD133 Rabbit mAb (NBP3-15310) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Immunohistochemistry-Paraffin-NBP3-15310-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts from normal (control) and CD133 knockout (KO) HCT116 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Knockout-Validated-NBP3-15310-img0001.jpg)
![Western Blot: CD133 Antibody (3Y4A5) [CD133] - Western blot analysis of lysates from HT-29 cells, using CD133 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517192943.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-522025172608.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517251995.jpg)
![Immunohistochemistry: STIM2 Antibody [NBP3-35783] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using STIM2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35783_rabbit-stim2-pab-912202415111893.jpg "Immunohistochemistry: STIM2 Antibody [NBP3-35783] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using STIM2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: STIM2 Antibody [NBP3-35783] - Western blot analysis of lysates from 293T cells, using STIM2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35783_rabbit-stim2-pab-91220241511187.jpg)
![Immunocytochemistry/ Immunofluorescence: STIM2 Antibody [NBP3-35783] - Immunofluorescence analysis of U-2 OS cells using STIM2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35783_rabbit-stim2-pab-912202414112846.jpg)
![Immunohistochemistry: STIM2 Antibody [NBP3-35783] - Immunohistochemistry analysis of paraffin-embedded Rat brain using STIM2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35783_rabbit-stim2-pab-912202415171178.jpg)
![Immunocytochemistry/ Immunofluorescence: STIM2 Antibody [NBP3-35783] - Immunofluorescence analysis of L929 cells using STIM2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35783_rabbit-stim2-pab-9122024159513.jpg)
![Flow Cytometry: CD63 Antibody (AD1) [NBP2-68077] - Figure A. A647 conjugated mouse anti rat CD63 and FITC conjugated mouse IgG1 isotype control. Figure B. A647 conjugated mouse anti rat CD63 and FITC conjugated mouse anti rat CD61.](http://images.novusbio.com/fullsize/CD63-Antibody-AD1-Flow-Cytometry-NBP2-68077-img0004.jpg "Flow Cytometry: CD63 Antibody (AD1) [NBP2-68077] - Figure A. A647 conjugated mouse anti rat CD63 and FITC conjugated mouse IgG1 isotype control. Figure B. A647 conjugated mouse anti rat CD63 and FITC conjugated mouse anti rat CD61.")
![Flow Cytometry: CD63 Antibody (AD1) [NBP2-68077] - Rat peripheral blood platelets stained with Mouse anti Rat CD63 Alexa Fluor(R)488.](http://images.novusbio.com/fullsize/CD63-Antibody-AD1-Flow-Cytometry-NBP2-68077-img0001.jpg)
![Flow Cytometry: CD63 Antibody (AD1) [NBP2-68077] - Rat peritoneal macrophages stained with Mouse anti Rat CD63:Alexa Fluor(R)647.](http://images.novusbio.com/fullsize/CD63-Antibody-AD1-Flow-Cytometry-NBP2-68077-img0002.jpg)
![Flow Cytometry: CD63 Antibody (AD1) [NBP2-68077] - Rat peripheral blood platelets stained with Mouse anti Rat CD63:FITC.](http://images.novusbio.com/fullsize/CD63-Antibody-AD1-Flow-Cytometry-NBP2-68077-img0003.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202414201354.jpg "Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202414201323.jpg)
![Western Blot: Coronin-1a Antibody [NBP3-38604] - Western blot analysis of various lysates using Coronin-1a Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202415152849.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202414123825.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202415152850.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-91220241412386.jpg)
![Immunohistochemistry: CD21 Antibody [NBP3-38538] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-9122024159579.jpg "Immunohistochemistry: CD21 Antibody [NBP3-38538] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody [NBP3-38538] - Immunofluorescence analysis of paraffin-embedded human spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-912202415171172.jpg)
![Western Blot: CD21 Antibody [NBP3-38538] - Western blot analysis of various lysates using CD21 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-912202414163018.jpg)
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody [NBP3-38538] - Immunofluorescence analysis of paraffin-embedded mouse spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-912202415121332.jpg)
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody [NBP3-38538] - Immunofluorescence analysis of paraffin-embedded rat spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-912202415171157.jpg)
![Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Western-Blot-NBP2-37388-img0003.jpg "Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of paraffin-embedded endometrium tissues using CD33 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Immunohistochemistry-NBP2-37388-img0007.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of HepG2 cells using CD33 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Flow-Cytometry-NBP2-37388-img0004.jpg)
![Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against human CD33 recombinant protein. (Expected MW is 49.2 kDa)](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Western-Blot-NBP2-37388-img0002.jpg)
![Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of paraffin-embedded esophageal cancer tissues using CD33 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Immunohistochemistry-NBP2-37388-img0005.jpg)
![ELISA: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-ELISA-NBP2-37388-img0001.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (3G1B3) [NBP2-61725] - Analysis using CD1C mAb against human CD1C (AA: extra 18-302) recombinant protein. (Expected MW is 58.1 kDa)](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-3G1B3-Western-Blot-NBP2-61725-img0004.jpg "Western Blot: CD1c/BDCA-1 Antibody (3G1B3) [NBP2-61725] - Analysis using CD1C mAb against human CD1C (AA: extra 18-302) recombinant protein. (Expected MW is 58.1 kDa)")
![Immunohistochemistry-Paraffin: CD1c/BDCA-1 Antibody (3G1B3) - BSA Free [NBP2-61725] - CD1c/BDCA-1 CODEX stain of human tonsil tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-3G1B3-BSA-Free-Immunohistochemistry-Paraffin-NBP2-61725-img0006.jpg)
![Flow Cytometry: CD1c/BDCA-1 Antibody (3G1B3) [NBP2-61725] - Analysis of Ramos cells using CD1C mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-3G1B3-Flow-Cytometry-NBP2-61725-img0002.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (3G1B3) [NBP2-61725] - Analysis using CD1C mAb against HEK293 (1) and CD1C (AA: extra 18-302)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-3G1B3-Western-Blot-NBP2-61725-img0005.jpg)
![Immunohistochemistry-Paraffin: CD1c/BDCA-1 Antibody (3G1B3) [NBP2-61725] - Analysis of rectum cancer tissues using CD1C mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-3G1B3-Immunohistochemistry-NBP2-61725-img0003.jpg)
![ELISA: CD1c/BDCA-1 Antibody (3G1B3) [NBP2-61725] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-3G1B3-ELISA-NBP2-61725-img0001.jpg)
![Immunohistochemistry-Frozen: CCL2/MCP1 Antibody (ECE.2) [NBP1-42312] - Detection of CCL2/MCP1 in mouse testis. 10x dilution, 200x magnification.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-ECE-2-Immunohistochemistry-Frozen-NBP1-42312-img0001.jpg "Immunohistochemistry-Frozen: CCL2/MCP1 Antibody (ECE.2) [NBP1-42312] - Detection of CCL2/MCP1 in mouse testis. 10x dilution, 200x magnification.")
![Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - HLymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NLS847-img0007.jpg "Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - HLymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - Analysis of anti-CCR7 antibody with lymph node at 6 ug/ml.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NLS847-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - Analysis of anti-CCR7 antibody with human bone, eosinophilic granuloma.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NLS847-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - Anti-CCR7 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NLS847-img0003.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - Hodgkin's disease Reed Sternberg cells](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NLS847-img0004.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - Brain Hypothalamus Neuron](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NLS847-img0005.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - Human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NLS847-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-immunocytochemistry-immunofluorescence-2972024113947..jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/1,000 dilution.Lane 1: Raji cell lysate (10 ug/Lane)Lane 2: Mouse lung tissue lysate (20 ug/Lane)Lane 3: Rat lung tissue lysate (20 ug/Lane)Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114129..jpg)
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/2,000 dilution.Lane 1: Daudi cell lysateLane 2: Raji cell lysateLane 3: Ramos cell lysateLane 4: Jurkat cell lysateLane 5: K-562 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 2 minutes 37 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114258..jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NBP2-31089] - Hodgkin's disease Reed Sternberg cells.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NBP2-31089-img0005.jpg "Immunohistochemistry-Paraffin: CCR7 Antibody [NBP2-31089] - Hodgkin's disease Reed Sternberg cells.")
![Immunohistochemistry-Paraffin: CCR7 Antibody [NBP2-31089] - Analysis of anti-CCR7 antibody with human tonsil.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NBP2-31089-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NBP2-31089] - Human lymph node tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NBP2-31089-img0003.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NBP2-31089] - Lymph node, eosinophilic granuloma Langerhans histiocytes.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NBP2-31089-img0004.jpg)