| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00303-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90685-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90585-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90077-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90037-50ul | |||
![Western Blot: IL-18/IL-1F4 Antibody (PSH02-08) [NBP3-32451] - Western blot analysis of IL-18/IL-1F4 on different lysates with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32451) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: A549 cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 1 minute 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32451) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32451_rabbit-il-18-il-1f4-mab-psh02-08-27820241633123.jpg "Western Blot: IL-18/IL-1F4 Antibody (PSH02-08) [NBP3-32451] - Western blot analysis of IL-18/IL-1F4 on different lysates with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32451) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: A549 cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 1 minute 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32451) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IL-18/IL-1F4 Antibody (PSH02-08) [NBP3-32451] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32451) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32451) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32451_rabbit-il-18-il-1f4-mab-psh02-08-298202414161830.jpg)
![Immunohistochemistry-Paraffin: ECSIT Antibody [NBP3-00303] - Immunochemical staining of human ECSIT in human brain with NBP3-00303 (1:25000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00303_rabbit-polyclonal-ecsit-antibody-2352025154439.png "Immunohistochemistry-Paraffin: ECSIT Antibody [NBP3-00303] - Immunochemical staining of human ECSIT in human brain with NBP3-00303 (1:25000, formalin-fixed paraffin embedded sections).")
![Western Blot: ECSIT Antibody [NBP3-00303] - NBP3-00303 at 1:500 dilution Lane A: NIH3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:49 kDa Observed band size:49 kDa](http://images.novusbio.com/fullsize/nbp3-00303_rabbit-polyclonal-ecsit-antibody-2352025213517.png)
![Flow Cytometry: IFN-gamma R1/CD119 Antibody (062) [NBP2-90685] - Flow cytometric analysis of Mouse IFNGR1(CD119) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse IFNGR1(CD119), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/IFN-gamma-R1-CD119-Antibody-062-Flow-Cytometry-NBP2-90685-img0002.jpg "Flow Cytometry: IFN-gamma R1/CD119 Antibody (062) [NBP2-90685] - Flow cytometric analysis of Mouse IFNGR1(CD119) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse IFNGR1(CD119), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/ Immunofluorescence: IFN-gamma R1/CD119 Antibody (062) [NBP2-90685] -staining of mouse IFN-gamma R1/CD119 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90685 (1:60) at 4C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-90685_rabbit-monoclonal-ifn-gamma-r1-cd119-antibody-062-235202521451779.png)
![Immunohistochemistry: HLA DP Antibody (PD00-70) [NBP3-32434] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HLA DP antibody (NBP3-32434) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32434) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32434_rabbit-hla-dp-mab-pd00-70-29820241420989.jpg "Immunohistochemistry: HLA DP Antibody (PD00-70) [NBP3-32434] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HLA DP antibody (NBP3-32434) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32434) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: HLA DP Antibody (PD00-70) [NBP3-32434] - Flow cytometric analysis of Daudi cells labeling HLA DP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32434, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32434_rabbit-hla-dp-mab-pd00-70-392024884637.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to blood vessel.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-020-Immunohistochemistry-Paraffin-NBP2-90586-img0002.jpg "Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to blood vessel.")
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse brain with rabbit monoclonal antibody (1:200). The image showing positive staining of choroid plexus.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-020-Immunohistochemistry-Paraffin-NBP2-90586-img0001.jpg)
![Flow Cytometry: Granzyme A Antibody (CB9) [NBP3-14619] - Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Granzyme A (CB9) purified antibody (concentration in sample 5,0 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Granzyme-A-Antibody-CB9-Flow-Cytometry-NBP3-14619-img0002.jpg "Flow Cytometry: Granzyme A Antibody (CB9) [NBP3-14619] - Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Granzyme A (CB9) purified antibody (concentration in sample 5,0 ug/ml, GAM APC).")
![Flow Cytometry: Granzyme A Antibody (CB9) [NBP3-14619] - Separation of human Granzyme A positive NK cells (red-filled) from Granzyme A negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Granzyme A (CB9) purified antibody (concentration in sample 5,0 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Granzyme-A-Antibody-CB9-Flow-Cytometry-NBP3-14619-img0001.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (009) [NBP2-90585] - Flow cytometric analysis of Mouse ICAM-1/CD54 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse ICAM-1/CD54, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-009-Flow-Cytometry-NBP2-90585-img0001.jpg "Flow Cytometry: ICAM-1/CD54 Antibody (009) [NBP2-90585] - Flow cytometric analysis of Mouse ICAM-1/CD54 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse ICAM-1/CD54, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (009) [NBP2-90585] - Immunofluorescence staining of Mouse ICAM-1/CD54 in Raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90585 antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.](http://images.novusbio.com/fullsize/nbp2-90585_rabbit-monoclonal-icam-1-cd54-antibody-009-23520252135170.png)
![Immunohistochemistry: CD47 Antibody (PD00-33) [NBP3-32156] - Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue with Rabbit anti-CD47 antibody (NBP3-32156) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32156_rabbit-cd47-mab-pd00-33-298202414161868.jpg "Immunohistochemistry: CD47 Antibody (PD00-33) [NBP3-32156] - Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue with Rabbit anti-CD47 antibody (NBP3-32156) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: CD47 Antibody (PD00-33) [NBP3-32156] - Flow cytometric analysis of Jurkat cells labeling CD47. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32156, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32156_rabbit-cd47-mab-pd00-33-392024884610.jpg)
![Western Blot: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Western blot analysis of Siglec-2/CD22 on Daudi cell lysates with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32136) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32136_rabbit-siglec-2-cd22-mab-po00-31-278202416331259.jpg "Western Blot: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Western blot analysis of Siglec-2/CD22 on Daudi cell lysates with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32136) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32136) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32136_rabbit-siglec-2-cd22-mab-po00-31-298202414115429.jpg)
![Western Blot: CD44 Antibody (065) [NBP2-90077] - Lane A: HL60 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:82 kDaObserved band size:82 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/CD44-Antibody-065-Western-Blot-NBP2-90077-img0001.jpg "Western Blot: CD44 Antibody (065) [NBP2-90077] - Lane A: HL60 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:82 kDaObserved band size:82 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human breast cancer with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-351-Immunohistochemistry-Paraffin-NBP2-90037-img0003.jpg "Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human breast cancer with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human melanoma with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-351-Immunohistochemistry-Paraffin-NBP2-90037-img0001.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human skin with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-351-Immunohistochemistry-Paraffin-NBP2-90037-img0002.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Surface staining pattern of human peripheral blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-S-HCL-1-Flow-Cytometry-NBP3-09167-img0001.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Surface staining pattern of human peripheral blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.")
![Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Separation of human CD22 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-S-HCL-1-Flow-Cytometry-NBP3-09167-img0002.jpg)
![Western Blot: Mouse Monoclonal Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - The reactivity of S-HCL-1 antibody was assessed by comparing binding signals in a panel of three human B cell and one T cell line. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji, Ramos, Daudi, and Jurkat cell lines, mixed and heated (100C, 5 min) without reducing agent in SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG2b monoclonal antibody S-HCL-1 (2 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green).CD22 was detected at ~110-125 kDa in Daudi cell line.](http://images.novusbio.com/fullsize/antibody/nbp3-09167_mouse-monoclonal-siglec-2-cd22-antibody-s-hcl-1-western-blot-11122024122650..jpg)