| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37449-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99099-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99524-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89884-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99098-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09027-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26498-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26186-50ul | |||
![Immunohistochemistry-Paraffin: PIAS2 Antibody [NB100-59734] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PIAS2-Antibody-Immunohistochemistry-Paraffin-NB100-59734-img0001.jpg "Immunohistochemistry-Paraffin: PIAS2 Antibody [NB100-59734] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: Tyrosylprotein Sulfotransferase 2/TPST2 Antibody [NBP3-03879] - Western blot analysis of extracts of various cell lines, using TPST2 antibody (NBP3-03879) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Tyrosylprotein Sulfotransferase 2-TPST2 Antibody-Western Blot-NBP3-03879-img0001.jpg "Western Blot: Tyrosylprotein Sulfotransferase 2/TPST2 Antibody [NBP3-03879] - Western blot analysis of extracts of various cell lines, using TPST2 antibody (NBP3-03879) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: Tyrosylprotein Sulfotransferase 2/TPST2 Antibody - Azide and BSA Free [Tyrosylprotein Sulfotransferase 2/TPST2] - Western blot analysis of lysates from U-2 OS cells using Tyrosylprotein Sulfotransferase 2/TPST2 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-03879_rabbit-polyclonal-tyrosylprotein-sulfotransferase-2-tpst2-antibody-5220251640299.jpg)
![Western Blot: RAG1 Antibody (1D9) [NBP2-37449] - Western blot analysis using RAG1 mAb against HEK293 (1) and RAG1 (AA: 818-868)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RAG1-Antibody-1D9-Western-Blot-NBP2-37449-img0003.jpg "Western Blot: RAG1 Antibody (1D9) [NBP2-37449] - Western blot analysis using RAG1 mAb against HEK293 (1) and RAG1 (AA: 818-868)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: RAG1 Antibody (1D9) [NBP2-37449] - Western blot analysis using RAG1 mAb against human RAG1 recombinant protein. (Expected MW is 31.6 kDa)](http://images.novusbio.com/fullsize/RAG1-Antibody-1D9-Western-Blot-NBP2-37449-img0002.jpg)
![ELISA: RAG1 Antibody (1D9) [NBP2-37449] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/RAG1-Antibody-1D9-ELISA-NBP2-37449-img0001.jpg)
![Western Blot: Clusterin/APOJ Antibody (PSH04-33) [NBP3-32196] - Western blot analysis of Clusterin/APOJ on different lysates with Rabbit anti-Clusterin/APOJ antibody (NBP3-32196) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: A431 cell lysate Lane 4: PANC-1 cell lysate Lane 5: MCF7 cell lysate Lane 6: T-47D cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 35-80 kDa Exposure time: 1 minute 50 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32196) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32196_rabbit-clusterin-apoj-mab-psh04-33-27820241521244.jpg "Western Blot: Clusterin/APOJ Antibody (PSH04-33) [NBP3-32196] - Western blot analysis of Clusterin/APOJ on different lysates with Rabbit anti-Clusterin/APOJ antibody (NBP3-32196) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: A431 cell lysate Lane 4: PANC-1 cell lysate Lane 5: MCF7 cell lysate Lane 6: T-47D cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 35-80 kDa Exposure time: 1 minute 50 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32196) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Clusterin/APOJ Antibody (PSH04-33) [NBP3-32196] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Clusterin/APOJ antibody (NBP3-32196) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32196) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32196_rabbit-clusterin-apoj-mab-psh04-33-29820241492623.jpg)
![Western Blot: CHD4 Antibody [NBP2-99099] - Anti-CHD4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: NIH3T3 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 218 kDa](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NBP2-99099-img0002.jpg "Western Blot: CHD4 Antibody [NBP2-99099] - Anti-CHD4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: NIH3T3 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 218 kDa")
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP2-99099] - Immunofluorescence staining of CHD4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99099 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-99099_rabbit-polyclonal-chd4-antibody-235202514371324.png)
![Western Blot: FAT10 Antibody [NBP2-99524] - Anti-FAT10 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 25 kDa](http://images.novusbio.com/fullsize/FAT10-Antibody-Western-Blot-NBP2-99524-img0003.jpg "Western Blot: FAT10 Antibody [NBP2-99524] - Anti-FAT10 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 25 kDa")
![Immunocytochemistry/Immunofluorescence: FAT10 Antibody [NBP2-99524] - Immunofluorescence staining of FAT10 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99524 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/nbp2-99524_rabbit-polyclonal-fat10-antibody-23520251628192.png)
![Immunohistochemistry-Paraffin: Clusterin/APOJ Antibody (210) [NBP2-89885] - Staining of human CLU in human appendix with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-210-Immunohistochemistry-Paraffin-NBP2-89885-img0003.jpg "Immunohistochemistry-Paraffin: Clusterin/APOJ Antibody (210) [NBP2-89885] - Staining of human CLU in human appendix with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: Clusterin/APOJ Antibody (210) [NBP2-89885] - Staining of human CLU in human pancreas with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-210-Immunohistochemistry-Paraffin-NBP2-89885-img0001.jpg)
![Immunohistochemistry-Paraffin: Clusterin/APOJ Antibody (210) [NBP2-89885] - Staining of human CLU in human ovarian cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-210-Immunohistochemistry-Paraffin-NBP2-89885-img0002.jpg)
![Flow Cytometry: Clusterin/APOJ Antibody (013) [NBP2-89884] - Analysis of Human CLU on A549 cells. Cells were treated, stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-013-Flow-Cytometry-NBP2-89884-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Clusterin/APOJ Antibody (013) [NBP2-89884] - Analysis of Human CLU on A549 cells. Cells were treated, stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (013) [NBP2-89884] - Staining of Human CLU in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89884 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89884_rabbit-monoclonal-clusterin-apoj-antibody-013-235202514371330.png)
![Immunohistochemistry-Paraffin: CHD4 Antibody [NBP2-99098] - Immunochemical staining of human CHD4 in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CHD4-Antibody-Immunohistochemistry-Paraffin-NBP2-99098-img0002.jpg "Immunohistochemistry-Paraffin: CHD4 Antibody [NBP2-99098] - Immunochemical staining of human CHD4 in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP2-99098] - Immunofluorescence staining of CHD4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99098 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-99098_rabbit-polyclonal-chd4-antibody-235202511444452.png)
![Flow Cytometry: IL-16 Antibody (14.1) - Chimeric [NBP3-09027] - Human peripheral blood leukocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 14.1 (NBP3-09027, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with NBP3-09027. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/IL-16-Antibody-14-1-Chimeric-Flow-Cytometry-NBP3-09027-img0001.jpg "Flow Cytometry: IL-16 Antibody (14.1) - Chimeric [NBP3-09027] - Human peripheral blood leukocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 14.1 (NBP3-09027, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with NBP3-09027. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.")
![Immunohistochemistry: ATF2 Antibody (3D12) [NBP3-26498] - Image of ATF2 Antibody (3D12) diluted at 1:115.5 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26498_rabbit-atf2-mab-3d12-262202414471538.jpg "Immunohistochemistry: ATF2 Antibody (3D12) [NBP3-26498] - Image of ATF2 Antibody (3D12) diluted at 1:115.5 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: ATF2 Antibody (3D12) [NBP3-26498] - Positive Western Blot detected in: K562 whole cell lysate, 293T whole cell lysate. All lanes: ATF2 Antibody at 1.2ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 36, 24, 49, 53, 14, 25, 16 KDa Observed band size: 70 KDa](http://images.novusbio.com/fullsize/nbp3-26498_rabbit-atf2-mab-3d12-2822024115253.jpg)
![Immunohistochemistry: c-jun Antibody (10D4) [NBP3-26186] - Image of c-jun Antibody (10D4) diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26186_rabbit-c-jun-mab-10d4-262202414471587.jpg "Immunohistochemistry: c-jun Antibody (10D4) [NBP3-26186] - Image of c-jun Antibody (10D4) diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: c-jun Antibody (10D4) [NBP3-26186] - Positive Western Blot detected in: 293 whole cell lysate. All lanes: c-jun Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36 kDa Observed band size: 43 kDa](http://images.novusbio.com/fullsize/nbp3-26186_rabbit-c-jun-mab-10d4-28220241144263.jpg)