| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92041-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54625-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52556-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37317-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89341-50ul | |||
![ELISA: Human C4 binding protein A ELISA Kit (Colorimetric) [NBP2-60550] - Standard curve.](http://images.novusbio.com/fullsize/Human-C4-binding-protein-A-ELISA-Kit-Colorimetric-ELISA-NBP2-60550-img0001.jpg "ELISA: Human C4 binding protein A ELISA Kit (Colorimetric) [NBP2-60550] - Standard curve.")
![Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of various cell lines, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Western-Blot-NBP3-16626-img0003.jpg "Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of various cell lines, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Immunohistochemistry of paraffin-embedded human placenta using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Immunohistochemistry-Paraffin-NBP3-16626-img0002.jpg)
![Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of Rat liver, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Western-Blot-NBP3-16626-img0004.jpg)
![Immunohistochemistry-Paraffin: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Immunohistochemistry of paraffin-embedded rat lung using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Immunohistochemistry-Paraffin-NBP3-16626-img0001.jpg)
![Western Blot: Bcl3 Antibody [NBP2-92041] - Analysis of extracts of various cell lines, using Bcl3 antibody (NBP2-92041) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Bcl3 Antibody-Western Blot-NBP2-92041-img0005.jpg "Western Blot: Bcl3 Antibody [NBP2-92041] - Analysis of extracts of various cell lines, using Bcl3 antibody (NBP2-92041) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Bcl3 Antibody [NBP2-92041] - Immunofluorescence analysis of C6 cells using Bcl3 Rabbit pAb (NBP2-92041) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Bcl3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92041-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Bcl3 Antibody [NBP2-92041] - Analysis of NIH/3T3 cells using BCL3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Bcl3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92041-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Bcl3 Antibody [NBP2-92041] - Immunofluorescence analysis of HeLa cells using Bcl3 Rabbit pAb (NBP2-92041) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Bcl3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92041-img0003.jpg)
![Western Blot: FAT10 Antibody [NBP2-81752] - Analysis of FAT10 in human testis tissue lysate with FAT10 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/FAT10-Antibody-Western-Blot-NBP2-81752-img0001.jpg "Western Blot: FAT10 Antibody [NBP2-81752] - Analysis of FAT10 in human testis tissue lysate with FAT10 antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: FAT10 Antibody - BSA Free [NBP2-81752] - Immunofluorescence of FAT10 in human testis tissue with FAT10 antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp2-81752_rabbit-polyclonal-fat10-antibody-2711202419333462.jpg)
![Immunohistochemistry: FAT10 Antibody - BSA Free [NBP2-81752] - Immunohistochemistry of FAT10 in human testis tissue with FAT10 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81752_rabbit-polyclonal-fat10-antibody-271120242022914.gif)
![Western Blot: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Analysis of human granulocyte lysate: Detection using 10 ug/ml Anti-CEACAM5 [5C8C4] followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-5C8C4-Western-Blot-NBP2-54625-img0008.jpg "Western Blot: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Analysis of human granulocyte lysate: Detection using 10 ug/ml Anti-CEACAM5 [5C8C4] followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.")
![Flow Cytometry: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM5 [5C8C4] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-5C8C4-Flow-Cytometry-NBP2-54625-img0003.jpg)
![ELISA: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Solid phase was coated with 3 ug/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM5/8 [5C8C4] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-5C8C4-ELISA-NBP2-54625-img0002.jpg)
![Immunoprecipitation: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Human granulocyte lysate: Utilizing 10 ug/ml Anti-CEACAM5/8 [5C8C4]. Detection of the sample using 5 ug/ml pAb Anti-CEA followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-5C8C4-Immunoprecipitation-NBP2-54625-img0006.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0003.jpg "Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)")
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of MCF-7 cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0007.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against HEK293 (1) and SELL (AA:83-186)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of Hela cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0006.jpg)
![ELISA: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-ELISA-NBP2-52556-img0005.jpg)
![Western Blot: TfR (Transferrin R) Antibody (1A1B2) [NBP2-37317] - Western blot analysis using TFRC mouse mAb against Jurkat (1), Hela (2), K562 (3), Cos7 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), HEK293 (8), RAJI (9) cell lysate.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-1A1B2-Western-Blot-NBP2-37317-img0004.jpg "Western Blot: TfR (Transferrin R) Antibody (1A1B2) [NBP2-37317] - Western blot analysis using TFRC mouse mAb against Jurkat (1), Hela (2), K562 (3), Cos7 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), HEK293 (8), RAJI (9) cell lysate.")
![Western Blot: TfR (Transferrin R) Antibody (1A1B2) [NBP2-37317] - Western blot analysis using TFRC mAb against human TFRC recombinant protein. (Expected MW is 39.7 kDa)](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-1A1B2-Western-Blot-NBP2-37317-img0002.jpg)
![Western Blot: TfR (Transferrin R) Antibody (1A1B2) [NBP2-37317] - Western blot analysis using TFRC mAb against HEK293 (1) and TFRC (AA: 608-727)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-1A1B2-Western-Blot-NBP2-37317-img0003.jpg)
![ELISA: TfR (Transferrin R) Antibody (1A1B2) [NBP2-37317] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-1A1B2-ELISA-NBP2-37317-img0001.jpg)
![Western Blot: MAVS Antibody (PSH0-30) [NBP3-32569] - Western blot analysis of MAVS on different lysates with Rabbit anti-MAVS antibody (NBP3-32569) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: C2C12 cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate Lane 5: A549 cell lysate Lane 6: Jurkat cell lysate Lane 7: THP-1 cell lysate Lane 8: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 56 kDa Observed band size: 75 kDa The molecular weight observed is consistent with that described in the literature (PMID: 16125763 and 30460894) Exposure time: 10S; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32569) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32569_rabbit-mavs-mab-psh0-30-278202415212477.png "Western Blot: MAVS Antibody (PSH0-30) [NBP3-32569] - Western blot analysis of MAVS on different lysates with Rabbit anti-MAVS antibody (NBP3-32569) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: C2C12 cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate Lane 5: A549 cell lysate Lane 6: Jurkat cell lysate Lane 7: THP-1 cell lysate Lane 8: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 56 kDa Observed band size: 75 kDa The molecular weight observed is consistent with that described in the literature (PMID: 16125763 and 30460894) Exposure time: 10S; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32569) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MAVS Antibody (PSH0-30) [NBP3-32569] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MAVS antibody (NBP3-32569) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32569) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX](http://images.novusbio.com/fullsize/nbp3-32569_rabbit-mavs-mab-psh0-30-298202414115412.jpg)
![Immunocytochemistry/ Immunofluorescence: MAVS Antibody (PSH0-30) [NBP3-32569] - Immunocytochemistry analysis of A431 cells labeling MAVSwith Rabbit anti-MAVS antibody (NBP3-32569) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MAVS antibody (NBP3-32569) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32569_rabbit-mavs-mab-psh0-30-29202410334019.png)
![Western Blot: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Western blot analysis of Neprilysin/CD10 on different lysates with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: Ramos cell lysate (20 ug/Lane) Lane 3: LNCaP cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) (negative) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32118_mouse-neprilysin-cd10-mab-a1g3-r-27820241631626.jpg "Western Blot: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Western blot analysis of Neprilysin/CD10 on different lysates with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: Ramos cell lysate (20 ug/Lane) Lane 3: LNCaP cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) (negative) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32118) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32118_mouse-neprilysin-cd10-mab-a1g3-r-298202414161875.jpg)
![Immunocytochemistry/ Immunofluorescence: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Immunocytochemistry analysis of Raji cells labeling Neprilysin/CD10 with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32118_mouse-neprilysin-cd10-mab-a1g3-r-29202410484874.jpg)
![Immunocytochemistry/Immunofluorescence: Latexin Antibody (101) [NBP2-89341] - Staining of Human LXN in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LXN monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Latexin-Antibody-101-Immunocytochemistry-Immunofluorescence-NBP2-89341-img0001.jpg "Immunocytochemistry/Immunofluorescence: Latexin Antibody (101) [NBP2-89341] - Staining of Human LXN in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LXN monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Latexin Antibody (101) [NBP2-89341] - Analysis of Human LXN expression on MCF-7 cells. The cells were treated , stained with purified anti-Human LXN, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Latexin-Antibody-101-Flow-Cytometry-NBP2-89341-img0002.jpg)
![Immunoprecipitation: Latexin Antibody (101) [NBP2-89341] - Lane A:0.5 mg A549 Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate2 uL anti-LXN rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-LXN rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 25.7 kDa Observed band size: 25.7 kDa](http://images.novusbio.com/fullsize/Latexin-Antibody-101-Immunoprecipitation-NBP2-89341-img0005.jpg)
![Western Blot: Latexin Antibody (101) [NBP2-89341] - NBP2-89341 at 1:500 dilution Lane A: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa Observed band size:26 kDa](http://images.novusbio.com/fullsize/nbp2-89341_rabbit-monoclonal-latexin-antibody-101-235202514492375.png)
![Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-278202415295563.jpg "Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32501) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-298202413585569.jpg)
![Immunocytochemistry/ Immunofluorescence: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunocytochemistry analysis of NIH/3T3 cells labeling KIF3A with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-29202410334094.jpg)
![Western Blot: MAVS Antibody (PSH01-65) [NBP3-32570] - Western blot analysis of MAVS on different lysates with Rabbit anti-MAVS antibody (NBP3-32570) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: Jurkat cell lysate Lane 5: THP-1 cell lysate Lane 6: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56.5 kDa Observed band size: 57/75 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32570) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32570_rabbit-mavs-mab-psh01-65-27820241631615.jpg "Western Blot: MAVS Antibody (PSH01-65) [NBP3-32570] - Western blot analysis of MAVS on different lysates with Rabbit anti-MAVS antibody (NBP3-32570) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: Jurkat cell lysate Lane 5: THP-1 cell lysate Lane 6: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56.5 kDa Observed band size: 57/75 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32570) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MAVS Antibody (PSH01-65) [NBP3-32570] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling MAVS with Rabbit anti-MAVS antibody (NBP3-32570) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32570, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32570_rabbit-mavs-mab-psh01-65-298202414262637.jpg)
![Immunohistochemistry: MAVS Antibody (PSH01-65) [NBP3-32570] - Immunohistochemical analysis of paraffin-embedded human kideny tissue with Rabbit anti-MAVS antibody (NBP3-32570) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32570) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32570_rabbit-mavs-mab-psh01-65-29820241492697.jpg)
![Immunocytochemistry/ Immunofluorescence: MAVS Antibody (PSH01-65) [NBP3-32570] - Immunocytochemistry analysis of A431 cells labeling MAVS with Rabbit anti-MAVS antibody (NBP3-32570) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MAVS antibody (NBP3-32570) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32570_rabbit-mavs-mab-psh01-65-2920241114838.jpg)