| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81083-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62562-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08990-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11967-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12009-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81105-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12006-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11985-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52641-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81084-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12078-0.025mg | |||
![Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083] - Flow-cytometry using the L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083]. Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 (NBP2-81083, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-MEL-14-Flow-Cytometry-NBP2-81083-img0001.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083] - Flow-cytometry using the L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083]. Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 (NBP2-81083, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) - Chimeric - Azide and BSA Free [NBP2-81083] - Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or NBP2-81083 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp2-81083_rabbit-monoclonal-l-selectin-cd62l-antibody-mel-14-74202522141216.png)
![Immunohistochemistry: CD55/DAF Antibody (LU30) - Chimeric [NBP2-62562] - Immunohistochemical staining of human lung tissue using anti-CD55 antibody. LU30 Anti-CD55 (Complement decay-accelerating factor)staining of paraffin embedded human lung tissue using the rabbit-chimeric version of LU30 (NBP2-62562). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62562 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-LU30-Chimeric-Immunohistochemistry-NBP2-62562-img0002.jpg "Immunohistochemistry: CD55/DAF Antibody (LU30) - Chimeric [NBP2-62562] - Immunohistochemical staining of human lung tissue using anti-CD55 antibody. LU30 Anti-CD55 (Complement decay-accelerating factor)staining of paraffin embedded human lung tissue using the rabbit-chimeric version of LU30 (NBP2-62562). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62562 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration, 2-4 ug/ml.")
![Flow Cytometry: CD55/DAF Antibody (LU30) - Chimeric [NBP2-62562] - Flow-cytometry using the anti-CD55 (Complement decay-accelerating factor) antibody LU-30. U93 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of LU-30 (NBP2-62562, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-LU30-Chimeric-Flow-Cytometry-NBP2-62562-img0001.jpg)
![Flow Cytometry: Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) [NBP3-08990] - Murine bone marrow-derived macrophages (BMDMs) were stained with anti-Siglec-1/CD169 antibody NBP3-08990 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor(R) 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a Siglec-1/CD169-positive population is apparent.](http://images.novusbio.com/fullsize/Siglec-1-CD169-Antibody-3D6-112-recombinant-version-Flow-Cytometry-NBP3-08990-img0001.jpg "Flow Cytometry: Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) [NBP3-08990] - Murine bone marrow-derived macrophages (BMDMs) were stained with anti-Siglec-1/CD169 antibody NBP3-08990 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor(R) 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a Siglec-1/CD169-positive population is apparent.")
![Immunocytochemistry/Immunofluorescence: CD37 Antibody (MB-1) [NBP3-11967] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells stained with the chimeric mouse IgG version of OX-126 (NBP3-11967) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11967, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD37-Antibody-MB-1-Immunocytochemistry-Immunofluorescence-NBP3-11967-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD37 Antibody (MB-1) [NBP3-11967] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells stained with the chimeric mouse IgG version of OX-126 (NBP3-11967) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11967, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: IL6R Antibody (D7715A7) - Chimeric [NBP3-12009] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of D7715A7 (NBP3-12009, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/IL6R-Antibody-D7715A7-Chimeric-Flow-Cytometry-NBP3-12009-img0002.jpg "Flow Cytometry: IL6R Antibody (D7715A7) - Chimeric [NBP3-12009] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of D7715A7 (NBP3-12009, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry /Immunofluorescence: IL6R Antibody (D7715A7) - Chimeric - Azide and BSA Free [NBP3-12009] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with NBP3-12009 at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP3-12009, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12009_rabbit-monoclonal-il6r-antibody-d7715a7-chimeric-74202522141243.png)
![Western Blot: Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) - Chimeric [NBP2-81105] - Western Blot using Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [NBP2-81105]. Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 3F12.2D3 [NBP2-81105] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kDa, though the form shown here is likely glycosylated. NBP2-81105 successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.](http://images.novusbio.com/fullsize/Lymphotoxin-alpha-TNF-beta-Antibody-3F12-2D3-Chimeric-Western-Blot-NBP2-81105-img0002.jpg "Western Blot: Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) - Chimeric [NBP2-81105] - Western Blot using Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [NBP2-81105]. Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 3F12.2D3 [NBP2-81105] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kDa, though the form shown here is likely glycosylated. NBP2-81105 successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.")
![Flow Cytometry: Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) - Chimeric [NBP2-81105] - Flow-cytometry using the Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [NBP2-81105]. U937 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of 3F12.2D3 (NBP2-81105, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Lymphotoxin-alpha-TNF-beta-Antibody-3F12-2D3-Chimeric-Flow-Cytometry-NBP2-81105-img0001.jpg)
![Western Blot: Integrin beta 4/CD104 Antibody (M126) [NBP3-23396] - Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin beta 4/CD104 (Tyr-1526) (lanes 1 & 2) and anti-Integrin beta 4/CD104 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin beta 4/CD104 (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23396_mouse-integrin-beta-4-cd104-mab-m126-19920231856926.jpg "Western Blot: Integrin beta 4/CD104 Antibody (M126) [NBP3-23396] - Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin beta 4/CD104 (Tyr-1526) (lanes 1 & 2) and anti-Integrin beta 4/CD104 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin beta 4/CD104 (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 Antibody (M126) [NBP3-23396] - Immunocytochemical labeling of Integrin beta 4/CD104 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-Integrin beta 4/CD104 (Cytoplasmic region) (left) or rabbit polyclonals anti-Integrin beta 4/CD104 (Tyr-1494) (middle) or anti-Integrin beta 4/CD104 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23396_mouse-integrin-beta-4-cd104-mab-m126-19920231992811.jpg)
![Immunocytochemistry/Immunofluorescence: beta-2 Adrenergic R/ADRB2 Antibody (R11/E1) - Chimeric [NBP3-12006] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of R11/E1 (NBP3-12006) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12006, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-R11-E1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12006-img0001.jpg "Immunocytochemistry/Immunofluorescence: beta-2 Adrenergic R/ADRB2 Antibody (R11/E1) - Chimeric [NBP3-12006] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of R11/E1 (NBP3-12006) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12006, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG-56) - Chimeric [NBP2-81084] - Flow-cytometry using [NBP2-81084]. Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of DREG-56 (NBP2-81084, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG-56-Chimeric-Flow-Cytometry-NBP2-81084-img0001.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (DREG-56) - Chimeric [NBP2-81084] - Flow-cytometry using [NBP2-81084]. Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of DREG-56 (NBP2-81084, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: CD59 Antibody (193-27) - Chimeric [NBP3-12078] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 193-27 (NBP3-12078) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12078, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD59-Antibody-193-27-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12078-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD59 Antibody (193-27) - Chimeric [NBP3-12078] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 193-27 (NBP3-12078) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12078, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: CD59 Antibody (193-27) - Chimeric - Azide and BSA Free [NBP3-12078] - Jurkat cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12078 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12078_rabbit-monoclonal-cd59-antibody-193-27-chimeric-74202522141239.png)