| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89884-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99524-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98729-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90060-50ul | |||
![Immunohistochemistry: CD47 Antibody (PD00-33) [NBP3-32156] - Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue with Rabbit anti-CD47 antibody (NBP3-32156) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32156_rabbit-cd47-mab-pd00-33-298202414161868.jpg "Immunohistochemistry: CD47 Antibody (PD00-33) [NBP3-32156] - Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue with Rabbit anti-CD47 antibody (NBP3-32156) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: CD47 Antibody (PD00-33) [NBP3-32156] - Flow cytometric analysis of Jurkat cells labeling CD47. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32156, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32156_rabbit-cd47-mab-pd00-33-392024884610.jpg)
![Flow Cytometry: Clusterin/APOJ Antibody (013) [NBP2-89884] - Analysis of Human CLU on A549 cells. Cells were treated, stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-013-Flow-Cytometry-NBP2-89884-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Clusterin/APOJ Antibody (013) [NBP2-89884] - Analysis of Human CLU on A549 cells. Cells were treated, stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (013) [NBP2-89884] - Staining of Human CLU in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89884 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89884_rabbit-monoclonal-clusterin-apoj-antibody-013-235202514371330.png)
![Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Western blot analysis of Thrombopoietin R/Tpo R on different lysates with Rabbit anti-Thrombopoietin R/Tpo R antibody (NBP3-33039) at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 ug/Lane) Lane 2: NIH/3T3 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (no heat) (20 ug/Lane) Lane 4: Rat spleen tissue lysate (40 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33039_rabbit-thrombopoietin-r-tpo-r-mab-je58-00-288202412493457.jpg "Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Western blot analysis of Thrombopoietin R/Tpo R on different lysates with Rabbit anti-Thrombopoietin R/Tpo R antibody (NBP3-33039) at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 ug/Lane) Lane 2: NIH/3T3 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (no heat) (20 ug/Lane) Lane 4: Rat spleen tissue lysate (40 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Flow cytometric analysis of A431 cells labeling Thrombopoietin R/Tpo R. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33039, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33039_rabbit-thrombopoietin-r-tpo-r-mab-je58-00-29202416372192.jpg)
![Western Blot: FAT10 Antibody [NBP2-99524] - Anti-FAT10 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 25 kDa](http://images.novusbio.com/fullsize/FAT10-Antibody-Western-Blot-NBP2-99524-img0003.jpg "Western Blot: FAT10 Antibody [NBP2-99524] - Anti-FAT10 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 25 kDa")
![Immunocytochemistry/Immunofluorescence: FAT10 Antibody [NBP2-99524] - Immunofluorescence staining of FAT10 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99524 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/nbp2-99524_rabbit-polyclonal-fat10-antibody-23520251628192.png)
![Western Blot: LAMP-2/CD107b Antibody (D3-D8-D3-R) [NBP3-32523] - Western blot analysis of LAMP-2/CD107b on different lysates with Mouse anti-LAMP-2/CD107b antibody (NBP3-32523) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Lane 4: HUVEC cell lysate Lane 5: JAR cell lysate Lane 6: HEK-293 cell lysate Lane 7: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 45 kDa Observed band size: 110 kDa Exposure time: 3 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32523) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32523_mouse-lamp-2-cd107b-mab-d3-d8-d3-r-278202416331251.jpg "Western Blot: LAMP-2/CD107b Antibody (D3-D8-D3-R) [NBP3-32523] - Western blot analysis of LAMP-2/CD107b on different lysates with Mouse anti-LAMP-2/CD107b antibody (NBP3-32523) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Lane 4: HUVEC cell lysate Lane 5: JAR cell lysate Lane 6: HEK-293 cell lysate Lane 7: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 45 kDa Observed band size: 110 kDa Exposure time: 3 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32523) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAMP-2/CD107b Antibody (D3-D8-D3-R) [NBP3-32523] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-LAMP-2/CD107b antibody (NBP3-32523) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32523) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32523_mouse-lamp-2-cd107b-mab-d3-d8-d3-r-298202414304116.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody [NBP2-98729] - Staining of mouse IRF6 in mouse small intestine with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/IRF6-Antibody-Immunohistochemistry-Paraffin-NBP2-98729-img0002.jpg "Immunohistochemistry-Paraffin: IRF6 Antibody [NBP2-98729] - Staining of mouse IRF6 in mouse small intestine with rabbit polyclonal antibody (1:1000).")
![Immunocytochemistry/Immunofluorescence: IRF6 Antibody [NBP2-98729] - Immunofluorescence staining of IRF6 in COS7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98729 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98729_rabbit-polyclonal-irf6-antibody-235202521451760.png)
![Immunohistochemistry: IgJ Antibody (PD00-89) [NBP3-32462] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IgJ antibody (NBP3-32462) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32462) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32462_rabbit-igj-mab-pd00-89-298202414304189.jpg "Immunohistochemistry: IgJ Antibody (PD00-89) [NBP3-32462] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IgJ antibody (NBP3-32462) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32462) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: IgJ Antibody (PD00-89) [NBP3-32462] - Flow cytometric analysis of Daudi cells labeling IgJ. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32462, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32462_rabbit-igj-mab-pd00-89-392024884639.jpg)
![Immunohistochemistry-Paraffin: ULBP-2 Antibody (022) [NBP2-90060] - Staining of human ULBP2 in human renal carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ULBP-2-Antibody-022-Immunohistochemistry-Paraffin-NBP2-90060-img0003.jpg "Immunohistochemistry-Paraffin: ULBP-2 Antibody (022) [NBP2-90060] - Staining of human ULBP2 in human renal carcinoma with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: ULBP-2 Antibody (022) [NBP2-90060] - Staining of human ULBP2 in human gastric cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ULBP-2-Antibody-022-Immunohistochemistry-Paraffin-NBP2-90060-img0004.jpg)
![Immunohistochemistry-Paraffin: ULBP-2 Antibody (022) [NBP2-90060] - Staining of human ULBP2 in human hepatoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ULBP-2-Antibody-022-Immunohistochemistry-Paraffin-NBP2-90060-img0002.jpg)
![Flow Cytometry: Nectin-2/CD112 Antibody (R2.525) [NBP3-09163] - Separation of human CD112 positive thrombocytes(red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD112 (R2.525) purified antibody (concentration in sample 9 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Nectin-2-CD112-Antibody-R2-525-Flow-Cytometry-NBP3-09163-img0002.jpg "Flow Cytometry: Nectin-2/CD112 Antibody (R2.525) [NBP3-09163] - Separation of human CD112 positive thrombocytes(red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD112 (R2.525) purified antibody (concentration in sample 9 ug/ml) GAM APC.")
![Flow Cytometry: Nectin-2/CD112 Antibody (R2.525) [NBP3-09163] - surface staining pattern of human peripheral whole blood stained using anti-human CD112 (R2.525) purified antibody (concentration in sample 9 ug/ml) GAM APC](http://images.novusbio.com/fullsize/Nectin-2-CD112-Antibody-R2-525-Flow-Cytometry-NBP3-09163-img0001.jpg)
![Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-MPO421-8B2-Flow-Intracellular-NBP2-41406-img0002.jpg "Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).")
![Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Intracellular staining pattern of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-MPO421-8B2-Flow-Intracellular-NBP2-41406-img0001.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to blood vessel.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-020-Immunohistochemistry-Paraffin-NBP2-90586-img0002.jpg "Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to blood vessel.")
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse brain with rabbit monoclonal antibody (1:200). The image showing positive staining of choroid plexus.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-020-Immunohistochemistry-Paraffin-NBP2-90586-img0001.jpg)
![Western Blot: CD79A Antibody (PDM0-23) [NBP3-32168] - Western blot analysis of CD79A on Raji cell lysates with Mouse anti-CD79A antibody (NBP3-32168) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32168) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32168_mouse-cd79a-mab-pdm0-23-278202415295589.jpg "Western Blot: CD79A Antibody (PDM0-23) [NBP3-32168] - Western blot analysis of CD79A on Raji cell lysates with Mouse anti-CD79A antibody (NBP3-32168) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32168) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD79A Antibody (PDM0-23) [NBP3-32168] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD79A antibody (NBP3-32168) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32168) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32168_mouse-cd79a-mab-pdm0-23-29820241435290.jpg)