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![Western Blot: HLA DOA Antibody [H00003111-B01P] - Analysis of HLA-DOA expression in transfected 293T cell line by HLA-DOA polyclonal antibody. Lane 1: HLA-DOA transfected lysate(27.61 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HLA-DOA-Antibody-Western-Blot-H00003111-B01P-img0002.jpg "Western Blot: HLA DOA Antibody [H00003111-B01P] - Analysis of HLA-DOA expression in transfected 293T cell line by HLA-DOA polyclonal antibody. Lane 1: HLA-DOA transfected lysate(27.61 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: HLA DOA Antibody [H00003111-B02P] - Analysis of HLA-DOA expression in transfected 293T cell line by HLA-DOA polyclonal antibody. Lane 1: HLA-DOA transfected lysate(27.5 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HLA-DOA-Antibody-Western-Blot-H00003111-B02P-img0002.jpg "Western Blot: HLA DOA Antibody [H00003111-B02P] - Analysis of HLA-DOA expression in transfected 293T cell line by HLA-DOA polyclonal antibody. Lane 1: HLA-DOA transfected lysate(27.5 KDa). Lane 2: Non-transfected lysate.")
![Flow Cytometry: IL-1 RI Antibody (10E2) [NBP3-26202] - Overlay Peak curve showing NIH/3T3 cells stained with IL-1 RI Antibody (10E2) (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26202_mouse-il-1-ri-mab-10e2-282202414183612.jpg "Flow Cytometry: IL-1 RI Antibody (10E2) [NBP3-26202] - Overlay Peak curve showing NIH/3T3 cells stained with IL-1 RI Antibody (10E2) (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.")
![Neutralization: IL-21 Antibody (MT216G/21.3m) [NBP3-18290] - The neutralizing capacity and ND50 was determined using ELISA andELISpot. A) HEK cells were transfected to express the IL-21 activation pathway with alkaline phosphatase as a reporter. Cells were stimulated in the presence of recombinant human IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After overnight incubation, enzyme activity was measured in ELISA. B) Human peripheral blood mononuclear cells (PBMC) were incubated with recombinant IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After 4 days the IgG-secreting B cells were quantified by ELISpot.](http://images.novusbio.com/fullsize/IL-21-Antibody-MT216G-21-3m-Neutralization-NBP3-18290-img0001.jpg "Neutralization: IL-21 Antibody (MT216G/21.3m) [NBP3-18290] - The neutralizing capacity and ND50 was determined using ELISA andELISpot. A) HEK cells were transfected to express the IL-21 activation pathway with alkaline phosphatase as a reporter. Cells were stimulated in the presence of recombinant human IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After overnight incubation, enzyme activity was measured in ELISA. B) Human peripheral blood mononuclear cells (PBMC) were incubated with recombinant IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After 4 days the IgG-secreting B cells were quantified by ELISpot.")
![Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Validation in Human HL-60 Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: IL-21 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp1-76740pep_il-21-antibody-blocking-peptide-2711202419251583.jpg "Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Validation in Human HL-60 Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: IL-21 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.")
![SDS-Page: HLA DOA Protein [NBP2-23211] - 3ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.](http://images.novusbio.com/fullsize/HLA-DOA-Protein-SDS-Page-NBP2-23211-img0001.jpg "SDS-Page: HLA DOA Protein [NBP2-23211] - 3ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.")
![Recombinant Human Calcineurin A Protein [NBP1-50945]](http://images.novusbio.com/fullsize/Recombinant-Human-Calcineurin-A-Protein-NBP1-50945-img0001.jpg "Recombinant Human Calcineurin A Protein [NBP1-50945]")
![SDS-Page: ECSIT Protein [NBP2-23017]](http://images.novusbio.com/fullsize/ECSIT-Protein-SDS-Page-NBP2-23017-img0001.jpg "SDS-Page: ECSIT Protein [NBP2-23017]")