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![Flow Cytometry: CD19 Antibody (HIB19) [NBP1-43436] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-43436. Staining of normal human peripheral blood cells with Anti-Human CD3 FITC and staining buffer (autofluorescence) (left) or Anti-Human CD19 PerCP-Cy5.5 (right).](http://images.novusbio.com/fullsize/CD19-Antibody-HIB19-Flow-Cytometry-NBP1-43436-img0003.jpg "Flow Cytometry: CD19 Antibody (HIB19) [NBP1-43436] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-43436. Staining of normal human peripheral blood cells with Anti-Human CD3 FITC and staining buffer (autofluorescence) (left) or Anti-Human CD19 PerCP-Cy5.5 (right).")
![Flow Cytometry: CD19 Antibody (HIB19) [NBP1-43436] - Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (left) and PE (right). Autofluorescence is indicated by open histogram. Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD19-Antibody-HIB19-Flow-Cytometry-NBP1-43436-img0001.jpg)
![Immunohistochemistry-Frozen: CD19 Antibody (SJ25-C1) [NBP1-28375] - CD19 (red) stained in human skin, counterstained with DAPI (blue) at 20x. Tissue fixed with 4% PFA, permeabilized with 0.5% Triton, and blocked with 37% Egg DI, 5% Milk DI, and 3% FSG 2% BSA TBS Ca/Azide in that order. Anti-CD19 applied at 1:500 v/v, 4C, O/N. Alexa Fluor 568 secondary antibody, with DAPI counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize/CD19-Antibody-SJ25-C1-Immunohistochemistry-Frozen-NBP1-28375-img0006.jpg "Immunohistochemistry-Frozen: CD19 Antibody (SJ25-C1) [NBP1-28375] - CD19 (red) stained in human skin, counterstained with DAPI (blue) at 20x. Tissue fixed with 4% PFA, permeabilized with 0.5% Triton, and blocked with 37% Egg DI, 5% Milk DI, and 3% FSG 2% BSA TBS Ca/Azide in that order. Anti-CD19 applied at 1:500 v/v, 4C, O/N. Alexa Fluor 568 secondary antibody, with DAPI counterstain. Image from verified customer review.")
![Flow Cytometry: CD19 Antibody (SJ25-C1) [NBP1-28375] - Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19-APC and Mouse Anti-Human CD3-FITC.](http://images.novusbio.com/fullsize/CD19-Antibody-SJ25-C1-Flow-Cytometry-NBP1-28375-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (2E2) [NBP2-22198] - Immunofluorescence analysis of HL-60(left) and K562 (right) cells using CD19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD19-Antibody-2E2-Immunocytochemistry-Immunofluorescence-NBP2-22198-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD19 Antibody (2E2) [NBP2-22198] - Immunofluorescence analysis of HL-60(left) and K562 (right) cells using CD19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.")
![Flow Cytometry: CD19 Antibody (2E2) [NBP2-22198] - Flow cytometric analysis of Raji cells using CD19 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/CD19-Antibody-2E2-Flow-Cytometry-NBP2-22198-img0001.jpg)
![Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (5A8) [NBP1-97639] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TNFSF13B-Antibody-5A8-Flow-Cytometry-NBP1-97639-img0001.jpg "Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (5A8) [NBP1-97639] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.")
![Western Blot: BCMA/TNFRSF17 Antibody (Vicky-2) [NBP1-97608] - Lane 1: MW marker, Lane 2: Mouse BCMA Recombinant Protein.](http://images.novusbio.com/fullsize/BCMA-TNFRSF17-Antibody-Vicky-2-Western-Blot-NBP1-97608-img0002.jpg "Western Blot: BCMA/TNFRSF17 Antibody (Vicky-2) [NBP1-97608] - Lane 1: MW marker, Lane 2: Mouse BCMA Recombinant Protein.")
![Western Blot: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Western blot analysis of Siglec-2/CD22 on Daudi cell lysates with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32136) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32136_rabbit-siglec-2-cd22-mab-po00-31-278202416331259.jpg "Western Blot: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Western blot analysis of Siglec-2/CD22 on Daudi cell lysates with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32136) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32136) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32136_rabbit-siglec-2-cd22-mab-po00-31-298202414115429.jpg)
![Western Blot: IRAK4 Antibody (JE41-77) [NBP3-32482] - Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody (NBP3-32482) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32482) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32482_rabbit-irak4-mab-je41-77-27820241631694.jpg "Western Blot: IRAK4 Antibody (JE41-77) [NBP3-32482] - Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody (NBP3-32482) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32482) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IRAK4 Antibody (JE41-77) [NBP3-32482] - Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-IRAK4 antibody (NBP3-32482) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32482) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32482_rabbit-irak4-mab-je41-77-298202413585559.jpg)