| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81113-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22400-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89960-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14193 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49160-25ul | |||
![Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Western blot analysis of Glutathione S-Transferase pi 1/GSTP1 on different lysates with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: JAR cell lysate Lane 8: Human brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-278202416331264.jpg "Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Western blot analysis of Glutathione S-Transferase pi 1/GSTP1 on different lysates with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: JAR cell lysate Lane 8: Human brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32411) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-29820241435285.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Immunocytochemistry analysis of HT-29 cells labeling Glutathione S-Transferase pi 1/GSTP1 with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-2920241192326.jpg)
![Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-V152-Flow-Cytometry-NBP3-07017-img0001.jpg "Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Western-Blot-NBP2-81113-img0002.jpg "Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.")
![Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Flow-cytometry using the Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R-125224 (NBP2-81113, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Flow-Cytometry-NBP2-81113-img0003.jpg)
![Immunocytochemistry /Immunofluorescence: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric - Azide and BSA Free [NBP2-81113] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton and stained with NBP2-81113 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81113, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81113_rabbit-monoclonal-fas-tnfrsf6-cd95-antibody-r-125224-chimeric-74202522141233.png)
![Western Blot: NAT10 Antibody (SR1073) [NBP3-22400] - Western blot analysis using NBP3-22400 in Hela cell lysate.](http://images.novusbio.com/fullsize/nbp3-22400_rabbit-nat10-mab-sr1073-57202317215168.jpg "Western Blot: NAT10 Antibody (SR1073) [NBP3-22400] - Western blot analysis using NBP3-22400 in Hela cell lysate.")
![SDS-Page: Recombinant Human Beclin 1 Protein [H00008678-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Beclin-1-Protein-SDS-Page-H00008678-P01-img0001.jpg "SDS-Page: Recombinant Human Beclin 1 Protein [H00008678-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: FXR1 Antibody (2G11) [H00008087-M01] - Analysis of FXR1 expression in transfected 293T cell line by FXR1 monoclonal antibody (M01), clone 2G11.Lane 1: FXR1 transfected lysate(61 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FXR1-Antibody-2G11-Western-Blot-H00008087-M01-img0007.jpg "Western Blot: FXR1 Antibody (2G11) [H00008087-M01] - Analysis of FXR1 expression in transfected 293T cell line by FXR1 monoclonal antibody (M01), clone 2G11.Lane 1: FXR1 transfected lysate(61 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: FXR1 Antibody (2G11) [H00008087-M01] - Analysis of monoclonal antibody to FXR1 on formalin-fixed paraffin-embedded human colon adenocarcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/FXR1-Antibody-2G11-Immunohistochemistry-Paraffin-H00008087-M01-img0005.jpg)
![Sandwich ELISA: FXR1 Antibody (2G11) [H00008087-M01] - Detection limit for recombinant GST tagged FXR1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FXR1-Antibody-2G11-Sandwich-ELISA-H00008087-M01-img0006.jpg)
![ELISA: Rat Beclin 1 ELISA Kit (Colorimetric) [NBP2-69960] - Samples were spiked with high concentrations of Rat Beclin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69960_rat-beclin-1-elisa-kit-colorimetric-13220241692216.jpg "ELISA: Rat Beclin 1 ELISA Kit (Colorimetric) [NBP2-69960] - Samples were spiked with high concentrations of Rat Beclin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Beclin 1 ELISA Kit (Colorimetric) [NBP2-69960] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69960_rat-beclin-1-elisa-kit-colorimetric-4320249115629.jpg)
![Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (TGM2/6943R) [NBP3-13790] - Formalin-fixed, paraffin-embedded human placenta stained with Transglutaminase 2/TGM2 antibody (TGM2/6943R). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/Transglutaminase-2-TGM2-Antibody-TGM2-6943R-Immunohistochemistry-Paraffin-NBP3-13790-img0001.jpg "Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (TGM2/6943R) [NBP3-13790] - Formalin-fixed, paraffin-embedded human placenta stained with Transglutaminase 2/TGM2 antibody (TGM2/6943R). Inset: PBS instead of primary antibody; secondary only negative control.")
![Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (TGM2/6943R) [NBP3-13790] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Transglutaminase 2/TGM2 antibody (TGM2/6943R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Transglutaminase-2-TGM2-Antibody-TGM2-6943R-Immunohistochemistry-Paraffin-NBP3-13790-img0002.jpg)
![Immunohistochemistry-Paraffin: Fas Ligand/TNFSF6 Antibody [NBP2-49160] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Fas-Ligand-TNFSF6-Antibody-Immunohistochemistry-Paraffin-NBP2-49160-img0004.jpg "Immunohistochemistry-Paraffin: Fas Ligand/TNFSF6 Antibody [NBP2-49160] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: Fas Ligand/TNFSF6 Antibody [NBP2-49160] - Staining of human lymph node shows distinct cytoplasmic positivity in subsets of cells.](http://images.novusbio.com/fullsize/Fas-Ligand-TNFSF6-Antibody-Immunohistochemistry-NBP2-49160-img0001.jpg)
![Immunohistochemistry-Paraffin: Fas Ligand/TNFSF6 Antibody [NBP2-49160] - Staining of human rectum shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Fas-Ligand-TNFSF6-Antibody-Immunohistochemistry-Paraffin-NBP2-49160-img0002.jpg)
![Immunohistochemistry-Paraffin: Fas Ligand/TNFSF6 Antibody [NBP2-49160] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Fas-Ligand-TNFSF6-Antibody-Immunohistochemistry-Paraffin-NBP2-49160-img0003.jpg)
![Western Blot: NAP1 Antibody [NBP1-26592] - Detection of Human and Mouse TAB3 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from 293T and (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAP1-Antibody-Western-Blot-NBP1-26592-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: NAP1 Antibody [NBP1-26592] - Detection of Human and Mouse TAB3 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from 293T and (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NAP1 Antibody [NBP1-26592] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. TAB3 was also immunoprecipitated by rabbit anti-TAB3 antibody NBP1-26593.](http://images.novusbio.com/fullsize/NAP1-Antibody-Western-Blot-NBP1-26592-img0001.jpg)
![Immunoprecipitation: NAP1 Antibody [NBP1-26592] - Detection of human TAB3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 (lot NBP1-26592-2) used for IP at 6 ug per reaction. TAB3 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-26592-1). For blotting immunoprecipitated TAB3, NBP1-26592 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 60 seconds.](http://images.novusbio.com/fullsize/NAP1-Antibody-Immunoprecipitation-NBP1-26592-img0004.jpg)