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![Western Blot: BCAM/CD239 Antibody (1G9W3) [NBP3-15879] - Western blot analysis of extracts of various cell lines, using BCAM/CD239 Rabbit mAb (NBP3-15879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/BCAM-CD239-Antibody-1G9W3-Western-Blot-NBP3-15879-img0002.jpg "Western Blot: BCAM/CD239 Antibody (1G9W3) [NBP3-15879] - Western blot analysis of extracts of various cell lines, using BCAM/CD239 Rabbit mAb (NBP3-15879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: BCAM/CD239 Antibody (1G9W3) [NBP3-15879] - Human oophoroma using BCAM/CD239 Rabbit mAb (NBP3-15879) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BCAM-CD239-Antibody-1G9W3-Immunohistochemistry-Paraffin-NBP3-15879-img0001.jpg)
![Western Blot: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Western blot analysis of ICAM-2/CD102 on different lysates with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse heart tissue lysate (no heat) Lane 3: Mouse kidney tissue lysate (no heat) Lane 4: Mouse kidney tissue lysate Lane 5: Mouse spleen tissue lysate (no heat) Lane 6: Rat spleen tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 31 kDa Observed band size: 45-50 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32456) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32456_rabbit-icam-2-cd102-mab-je63-28-278202416285280.jpg "Western Blot: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Western blot analysis of ICAM-2/CD102 on different lysates with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse heart tissue lysate (no heat) Lane 3: Mouse kidney tissue lysate (no heat) Lane 4: Mouse kidney tissue lysate Lane 5: Mouse spleen tissue lysate (no heat) Lane 6: Rat spleen tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 31 kDa Observed band size: 45-50 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32456) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32456) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32456_rabbit-icam-2-cd102-mab-je63-28-298202414262629.jpg)
![Western Blot: Nectin-3/PVRL3 Antibody [NBP2-20013] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nectin 3 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Nectin-3-PVRL3-Antibody-Western-Blot-NBP2-20013-img0002.jpg "Western Blot: Nectin-3/PVRL3 Antibody [NBP2-20013] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nectin 3 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: PVRL3 Antibody [NBP2-20013] - Sample (30 ug of whole cell lysate) A: THP-1 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/PVRL3-Antibody-Western-Blot-NBP2-20013-img0001.jpg)
![Western Blot: ICAM-2/CD102 Antibody [NBP1-31612] - Sample(30 ug whole cell lysate)A:HeLa S3 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Western-Blot-NBP1-31612-img0005.jpg "Western Blot: ICAM-2/CD102 Antibody [NBP1-31612] - Sample(30 ug whole cell lysate)A:HeLa S3 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ICAM-2/CD102 Antibody [NBP1-31612] - Paraffin-embedded breast cancer, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Immunohistochemistry-Paraffin-NBP1-31612-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD6 Antibody (SPV-L14) [NBP2-44556] - Immunofluorescence staining of MOLT4 cells using CD6 Antibody (SPV-L14) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/CD6-Antibody-SPV-L14-Immunocytochemistry-Immunofluorescence-NBP2-44556-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD6 Antibody (SPV-L14) [NBP2-44556] - Immunofluorescence staining of MOLT4 cells using CD6 Antibody (SPV-L14) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.")
![Immunoprecipitation: ZEB1 Antibody [NBP3-14694] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-ZEB1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. ZEB1 was alsoimmunoprecipitated by rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1,was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunoprecipitation-NBP3-14694-img0001.jpg "Immunoprecipitation: ZEB1 Antibody [NBP3-14694] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-ZEB1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. ZEB1 was alsoimmunoprecipitated by rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1,was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.")
![Western Blot: Antibody (AT2G10) [NBP2-88839] - The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. Jurkat cell lysate](http://images.novusbio.com/fullsize/Antibody-AT2G10-Western-Blot-NBP2-88839-img0002.jpg "Western Blot: Antibody (AT2G10) [NBP2-88839] - The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. Jurkat cell lysate")
![Flow Cytometry: Antibody (AT2G10) [NBP2-88839] - Flow cytometry analysis of Rac1,2,3 in Jurkat cells. The cell was stained with at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/Antibody-AT2G10-Flow-Cytometry-NBP2-88839-img0003.jpg)
![Western Blot: Antibody (AT2G10) [NBP2-88839] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human Rac 1 proteinLane 2.: Recombinant human Rac 2 proteinLane 3.: Recombinant human Rac 3 protein](http://images.novusbio.com/fullsize/Antibody-AT2G10-Western-Blot-NBP2-88839-img0001.jpg)
![Western Blot: ZEB1 Antibody (JE57-64) [NBP3-33111] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33111) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HUVEC cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33111) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33111_rabbit-zeb1-mab-je57-64-288202414191510.jpg "Western Blot: ZEB1 Antibody (JE57-64) [NBP3-33111] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33111) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HUVEC cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33111) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ZEB1 Antibody (JE57-64) [NBP3-33111] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-ZEB1 antibody (NBP3-33111) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33111) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33111_rabbit-zeb1-mab-je57-64-29820241492615.jpg)
![Flow Cytometry: CD99 Antibody (3B2/TA8) [NBP3-09116] - Mass cytometry (surface staining) of human peripheral blood cells (after separation using Ficoll-Paque density gradient centrifugation) with anti-human CD99 (3B2/TA8) Eu153. Gated on singlets.](http://images.novusbio.com/fullsize/CD99-Antibody-3B2-TA8-Flow-Cytometry-NBP3-09116-img0003.jpg "Flow Cytometry: CD99 Antibody (3B2/TA8) [NBP3-09116] - Mass cytometry (surface staining) of human peripheral blood cells (after separation using Ficoll-Paque density gradient centrifugation) with anti-human CD99 (3B2/TA8) Eu153. Gated on singlets.")
![Flow Cytometry: CD99 Antibody (3B2/TA8) [NBP3-09116] - Surface staining pattern of human peripheral blood stained using anti-human CD99 (3B2/TA8) purified antibody (concentration in sample 2 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD99-Antibody-3B2-TA8-Flow-Cytometry-NBP3-09116-img0001.jpg)
![Flow Cytometry: CD99 Antibody (3B2/TA8) [NBP3-09116] - Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD99 (3B2/TA8) purified antibody (concentration in sample 2 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD99-Antibody-3B2-TA8-Flow-Cytometry-NBP3-09116-img0002.jpg)
![Flow Cytometry: CD48/SLAMF2 Antibody (341) [NBP2-90577] - Flow cytometric analysis of Mouse CD48/SLAMF2 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD48/SLAMF2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CD48-SLAMF2-Antibody-341-Flow-Cytometry-NBP2-90577-img0001.jpg "Flow Cytometry: CD48/SLAMF2 Antibody (341) [NBP2-90577] - Flow cytometric analysis of Mouse CD48/SLAMF2 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD48/SLAMF2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: CD48/SLAMF2 Antibody (341) [NBP2-90577] - Immunofluorescence staining of Mouse CD48/SLAMF2 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90577 antibody (1:60) at 37C 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/nbp2-90577_rabbit-monoclonal-cd48-slamf2-antibody-341-235202514331563.png)