| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59497-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89345-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05047-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-21619-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79538-5Plates | |||
![Western Blot: Leptin/OB Antibody [NBP1-59324] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-Western-Blot-NBP1-59324-img0006.jpg "Western Blot: Leptin/OB Antibody [NBP1-59324] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.")
![Immunohistochemistry-Paraffin: Leptin/OB Antibody [NBP1-59324] - Human pancreas tissue, 4-8ug/ml.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-Immunohistochemistry-Paraffin-NBP1-59324-img0005.jpg)
![Simple Western: Leptin/OB Antibody [NBP1-59324] - Simple Western analysis of Leptin. Lane 1- biotinylated ladder; Lane 2- negative control (non-transfected MIN6 cells, 0.2 mg/ml); Lane 3- MIN6 cells transfected with plasmid expressing mouse leptin (positive control, 0.2 mg/ml). Image from verified customer review.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-Simple-Western-NBP1-59324-img0007.jpg)
![Western Blot: Leptin/OB Antibody [NB300-611] - Analysis of leptin in mouse 3T3-L1.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-Western-Blot-NB300-611-img0005.jpg "Western Blot: Leptin/OB Antibody [NB300-611] - Analysis of leptin in mouse 3T3-L1.")
![Immunohistochemistry-Frozen: Leptin/OB Antibody [NB300-611] - Staining of Leptin in sheep brain.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-Immunohistochemistry-Frozen-NB300-611-img0004.jpg)
![Western Blot: Leptin Antibody [NBP1-59320] - Titration: 0.2-1 ug/ml, Positive Control: Human Small Intestine.](http://images.novusbio.com/fullsize/Leptin-Antibody-Western-Blot-NBP1-59320-img0001.jpg "Western Blot: Leptin Antibody [NBP1-59320] - Titration: 0.2-1 ug/ml, Positive Control: Human Small Intestine.")
![Immunohistochemistry-Paraffin: Leptin Antibody [NBP1-59320] - Human placenta tissue at an antibody concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/Leptin-Antibody-Immunohistochemistry-Paraffin-NBP1-59320-img0003.jpg)
![Western Blot: Leptin/OB Antibody (7E10) [NBP2-59497] - Lane 1: 293T cell lysates, Lane 2: Leptin transfected 293T cell lysates](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-7E10-Western-Blot-NBP2-59497-img0001.jpg "Western Blot: Leptin/OB Antibody (7E10) [NBP2-59497] - Lane 1: 293T cell lysates, Lane 2: Leptin transfected 293T cell lysates")
![Immunocytochemistry/Immunofluorescence: Leptin/OB Antibody (7E10) [NBP2-59497] - Analysis of Leptin in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Leptin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-7E10-Immunocytochemistry-Immunofluorescence-NBP2-59497-img0002.jpg)
![Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-002-Flow-Cytometry-NBP2-89345-img0001.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Leptin/OB Antibody (002) [NBP2-89345] - Staining of LEP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-89345 (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Cytoplasm .](http://images.novusbio.com/fullsize/nbp2-89345_rabbit-monoclonal-leptin-ob-antibody-002-23520251129371.png)
![SDS-Page: Human Leptin/OB Protein [NBP2-34934]](http://images.novusbio.com/fullsize/Human-Leptin-OB-Protein-SDS-Page-NBP2-34934-img0001.jpg "SDS-Page: Human Leptin/OB Protein [NBP2-34934]")
![ELISA: Leptin/OB Antibody (11) - BSA Free [NBP1-05047] - The calibration curve of a sandwich assay for Leptin using NBP2-21618 as the capture antibody and NBP2-23586 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-11-BSA-Free-ELISA-NBP1-05047-img0001.jpg "ELISA: Leptin/OB Antibody (11) - BSA Free [NBP1-05047] - The calibration curve of a sandwich assay for Leptin using NBP2-21618 as the capture antibody and NBP2-23586 as the biotinylated detection antibody.")
![ELISA: Leptin/OB ELISA Kit [HRP] [KA0026] - Standard curve](http://images.novusbio.com/fullsize/Leptin-OB-ELISA-Kit-[HRP]-ELISA-KA0026-img0001.jpg "ELISA: Leptin/OB ELISA Kit [HRP] [KA0026] - Standard curve")
![Sandwich ELISA: Leptin/OB Antibody Pair [HRP] [NBP2-79538] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79538-img0001.jpg "Sandwich ELISA: Leptin/OB Antibody Pair [HRP] [NBP2-79538] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")