| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16893-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59441-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15579-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92974-0.02ml | |||
![Western Blot: HO-2/HMOX2 Antibody [NBP1-36745] - Analysis of K562 cell lysate (35ug protein in RIPA buffer) using NBP1-36745 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/HO-2-HMOX2-Antibody-Western-Blot-NBP1-36745-img0003.jpg "Western Blot: HO-2/HMOX2 Antibody [NBP1-36745] - Analysis of K562 cell lysate (35ug protein in RIPA buffer) using NBP1-36745 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: HIF-2 alpha Overexpression Lysate (Adult Normal) [NBL1-10286] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIF-2 alpha.](http://images.novusbio.com/fullsize/HIF-2-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10286-img0002.jpg "Western Blot: HIF-2 alpha Overexpression Lysate (Adult Normal) [NBL1-10286] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIF-2 alpha.")
![Western Blot: VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody [NBP3-16893] - Western blot analysis of extracts of NIH/3T3 cells, using VEGFR2/KDR/Flk-1 antibody (NBP3-16893) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-[p-Tyr1175]-Antibody-Western-Blot-NBP3-16893-img0001.jpg "Western Blot: VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody [NBP3-16893] - Western blot analysis of extracts of NIH/3T3 cells, using VEGFR2/KDR/Flk-1 antibody (NBP3-16893) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: VEGFR2/KDR/Flk-1 [p Tyr1175] Antibody - Azide and BSA Free [NBP3-16893] - Western blot analysis of lysates from A-431 cells, using VEGFR2/KDR/Flk-1 Rabbit pAb at 1:500 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-16893_rabbit-vegfr2-kdr-flk-1-p-tyr-1175-pab-52202511374126.jpg)
![Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/AKT1-2-3-p-Ser473-Antibody-2E3A3-Western-Blot-NBP3-16948-img0001.jpg "Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of NIH/3T3 cells, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AKT1-2-3-p-Ser473-Antibody-2E3A3-Western-Blot-NBP3-16948-img0002.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody (14D7) [NBP2-59441] - Lane 1: Recombinant LDHB protein, Lane 2: A549 cell lysates, Lane 3: 293T cell lysates, Lane 4: K562 cell lysates, Lane 5: Jurkat cell lysates](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-14D7-Western-Blot-NBP2-59441-img0001.jpg "Western Blot: Lactate Dehydrogenase B Antibody (14D7) [NBP2-59441] - Lane 1: Recombinant LDHB protein, Lane 2: A549 cell lysates, Lane 3: 293T cell lysates, Lane 4: K562 cell lysates, Lane 5: Jurkat cell lysates")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody (14D7) [NBP2-59441] - Analysis of LDHB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LDHB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-14D7-Immunocytochemistry-Immunofluorescence-NBP2-59441-img0002.jpg)
![Western Blot: CREB [p Ser129] Antibody [NBP3-05463] - Western blot analysis of extracts of 293T cells, using CREB antibody (NBP3-05463) at 1:500 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CREB-[p-Ser129]-Antibody-Western-Blot-NBP3-05463-img0001.jpg "Western Blot: CREB [p Ser129] Antibody [NBP3-05463] - Western blot analysis of extracts of 293T cells, using CREB antibody (NBP3-05463) at 1:500 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CREB [p Ser129] Antibody [NBP3-05463] - Western blot analysis of extracts of C6 cells, using CREB antibody (NBP3-05463) at 1:500 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CREB [p Ser129] Antibody-Western Blot-NBP3-05463-img0002.jpg)
![SDS-Page: Recombinant Human ERK2 Protein [NBP1-30309] - 15% SDS-Page (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-ERK2-Protein-SDS-Page-NBP1-30309-img0001.jpg "SDS-Page: Recombinant Human ERK2 Protein [NBP1-30309] - 15% SDS-Page (3ug)")
![SDS-Page: Recombinant Human AKT1 Protein [NBP2-52121] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant-Human-AKT1-Protein-SDS-Page-NBP2-52121-img0001.jpg "SDS-Page: Recombinant Human AKT1 Protein [NBP2-52121] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain")
![Western Blot: GSK-3 alpha Antibody [NBP3-15579] - Western blot analysis of extracts of various cell lines, using GSK-3 alpha antibody (NBP3-15579) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Western-Blot-NBP3-15579-img0002.jpg "Western Blot: GSK-3 alpha Antibody [NBP3-15579] - Western blot analysis of extracts of various cell lines, using GSK-3 alpha antibody (NBP3-15579) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 alpha Antibody [NBP3-15579] - Western blot analysis of extracts from normal (control) and GSK-3 alpha knockout (KO) HeLa cells, using GSK-3 alpha antibody (18655) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Knockout-Validated-NBP3-15579-img0001.jpg)
![Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution or GSK3alpha antibody (A0645).293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-[p-Ser21]-Antibody-Western-Blot-NBP3-05443-img0003.jpg "Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution or GSK3alpha antibody (A0645).293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 and HeLa cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-[p-Ser21]-Antibody-Western-Blot-NBP3-05443-img0002.jpg)
![Immunoprecipitation: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug GSK-3 alpha/beta pAb (NBP3-05443). Western blot was performed from the immunoprecipitate using GSK-3 alpha/beta pAb (NBP3-05443) at a dilution of 1:1000. 293T cells were treated by Insulin (100 nM) at 37C for 10 minutes after serum-starvation overnight.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-[p-Ser21]-Antibody-Immunoprecipitation-NBP3-05443-img0001.jpg)
![Western Blot: BMAL1 Antibody (4F5N3) [NBP3-16467] - Western blot analysis of extracts of various cell lines, using BMAL1 Rabbit mAb (NBP3-16467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/BMAL1-Antibody-4F5N3-Western-Blot-NBP3-16467-img0001.jpg "Western Blot: BMAL1 Antibody (4F5N3) [NBP3-16467] - Western blot analysis of extracts of various cell lines, using BMAL1 Rabbit mAb (NBP3-16467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: BMAL1 Antibody (4F5N3) [NBP3-16467] - Western blot analysis of various lysates using BMAL1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-16467_rabbit-bmal1-mab-4f5n3-522025125450.jpg)
![Western Blot: EGLN2/PHD1 Antibody [NBP2-92974] - Analysis of extracts of various cell lines, using EGLN2/PHD1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/EGLN2-PHD1-Antibody-Western-Blot-NBP2-92974-img0002.jpg "Western Blot: EGLN2/PHD1 Antibody [NBP2-92974] - Analysis of extracts of various cell lines, using EGLN2/PHD1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunoprecipitation: EGLN2/PHD1 Antibody - BSA Free [NBP2-92974] - Immunoprecipitation analysis of 150 ug extracts of HeLa cells using 3 ug Prolyl hydroxylase PHD1 (EGLN2/PHD1) antibody . Western blot was performed from the immunoprecipitate using Prolyl hydroxylase PHD1 (EGLN2/PHD1) antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp2-92974_rabbit-polyclonal-egln2-phd1-antibody-42202514275270.jpg)